MA5-14674

antibody from Invitrogen Antibodies

Targeting: CEACAM5 CD66e, CEA

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Flow cytometry (Supportive data in Antibodypedia)

Radioimmunoassay (Recommended by provider)

Antibody Data

Product number

MA5-14674

Provider

Invitrogen Antibodies

Product name

Anti-CEA Monoclonal Antibody (1105)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

The predicted MW of CEA is ~77kD, but by Western blot MA5-14675 detects CEA with varying degrees of glycosylation at ~77-180kD. Product MA514675 is a smaller package size of MIC0102 (formerly sold as a Seradyn product).

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

1105

Vial size

100 ug

Concentration

1 mg/ml

Storage

-20° C, Avoid Freeze/Thaw Cycles

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Carcinoembryonic Antigen (CEA, green) in BxPC-3 cells. Cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100, and blocked with 0.3% BSA in PBS, each for 15 minutes at room temperature. Cells were stained with a CEA monoclonal antibody (Product # MA5-14674) at a dilution of 10 µg/mL in blocking buffer for 1 hour at room temperature, and then incubated with a goat anti-mouse IgG Superclonal™ secondary antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:1000 for 1 hour at room temperature. Nuclei (blue) were stained with Hoechst nuclear stain. Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of Carcinoembryonic Antigen (CEA) on BxPC-3 cells. Cells were harvested with 0.25% Trypsin-EDTA and stained with a CEA monoclonal antibody (Product # MA5-14674) at a dilution of 10 µg/mL (pink histogram), or with a mouse isotype control (black histogram) at a dilution of 10 µg/mL in 5% FCS in PBS. After incubation of the primary antibody for 1 hour on ice, the cells were stained with a goat anti-mouse IgG secondary antibody, DyLight 488 conjugate (Product # 35502) at a dilution of 1:40 for 1 hour on ice. A representative 10,000 cells were acquired for each sample.