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- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Flow cytometry [2]
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- Product number
- ABIN108734 - Provider product page
- Provider
- antibodies-online
- Proper citation
- Antibodies-Online Cat#ABIN108734, RRID:AB_10956734
- Product name
- anti-Carcinoembryonic Antigen-Related Cell Adhesion Molecule 5 (CEACAM5) antibody
- Antibody type
- Monoclonal
- Antigen
- immunisation with extracted protein of CEACAM5
- Description
- Protein G
- Reactivity
- Human
- Isotype
- IgG
- Antibody clone number
- 26-5-1
- Vial size
- 100 μg
- Concentration
- 1 mg/mL
- Storage
- short term: 2°C - 8°C, long term: -20°C
- Handling
- Avoid repeated freezing and thawing.
Submitted references Thermo-stability and antitumor activity on colon cancer cell lines of monoclonal anti-CEA antibody-saporin immunotoxin.
Antigenic sites in carcinoembryonic antigen.
Effects of human bone marrow stroma on the growth of human tumor cells.
Two CEA and three NCA species, although distinguishable by monoclonal antibodies, have nearly identical peptide patterns.
Manosroi J, von Kleist S, Manosroi A, Grunert F
Journal of Korean medical science 1992 Jun;7(2):128-35
Journal of Korean medical science 1992 Jun;7(2):128-35
Antigenic sites in carcinoembryonic antigen.
Hammarstrom S, Shively JE, Paxton RJ, Beatty BG, Larsson A, Ghosh R, Bormer O, Buchegger F, Mach JP, Burtin P
Cancer research 1989 Sep 1;49(17):4852-8
Cancer research 1989 Sep 1;49(17):4852-8
Effects of human bone marrow stroma on the growth of human tumor cells.
Strobel ES, Strobel HG, Bross KJ, Winterhalter B, Fiebig HH, Schildge JU, Löhr GW
Cancer research 1989 Feb 15;49(4):1001-7
Cancer research 1989 Feb 15;49(4):1001-7
Two CEA and three NCA species, although distinguishable by monoclonal antibodies, have nearly identical peptide patterns.
Grunert F, AbuHarfeil N, Schwarz K, von Kleist S
International journal of cancer. Journal international du cancer 1985 Sep 15;36(3):357-62
International journal of cancer. Journal international du cancer 1985 Sep 15;36(3):357-62
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Members of the CEA family were expressed on BOSC cells after transient transfection with expression vectors containing either the cDNA of CEACAM1, 5, 6 or 8. Recognition of CEACAM3 and of a recombinant transmembrane-anchored PSG1 fusion protein was tested on stably transfected HeLa cells. Expression of the constructs was confirmed with monoclonal antibodies known to recognize the corresponding proteins (CEACAM1, 3, 5 and 6: D14HD11; CEACAM8: 80H3; PSG: BAP1; green curves). An irrelevant monoclonal antibody served as a negative control (black curves). For specificity testing, protein G purified 26/5/1 was tested on all CEACAM transfectants. A positive signal was only obtained with CEACAM5 expressing cells (red curves).
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- FACS analysis of BOSC23 cells using 26/5/1. BOSC23 cells were transiently transfected with an expres-sion vector encoding either CEACAM5 (red curve) or an irrelevant protein (control transfectant). Binding of 26/5/1 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with CEACAM5 transfected cells.
