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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-15275 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PI4K2A Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Vial size
- 400 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Coupling of microtubule motors with AP-3 generated organelles in axons by NEEP21 family member calcyon.
Shi L, Hines T, Bergson C, Smith D
Molecular biology of the cell 2018 Aug 15;29(17):2055-2068
Molecular biology of the cell 2018 Aug 15;29(17):2055-2068
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot analysis using a PI4K2A polyclonal antibody (Product # PA5-15275) in mouse brain tissue lysates (35 µg per lane).
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot analysis using a PI4K2A polyclonal antibody (Product # PA5-15275) in HL-60 cell lysate. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis using a PI4K2A polyclonal antibody (Product # PA5-15275) on HeLa cells. 0.025 mg/mL primary antibody was followed by fluor-conjugated donkey anti-rabbit lgG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- Immunohistochemical analysis of formalin-fixed, paraffin-embedded human cancer tissue using a PI4K2A polyclonal antibody (Product # PA5-15275), followed by HRP-conjugated secondary antibody and DAB staining.
Supportive validation
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- Invitrogen Antibodies (provider)
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- FIGURE 4: Caly sorting requires interaction with adaptor protein complexes. (A-C) Differential localization of Caly-WT and Caly-ATEA in EE/SEs and LE/LROs. Confocal micrographs of DRG axons transfected with mCh-Caly-WT or mCh-Caly-ATEA (red) and stained with LAMP1 (A) or PI4KIIalpha (B) antibodies or cotransfected with GFP-Rab5 (C) (green). Bar graphs show the mean and SEM of overlapping red and green puncta in 100-mum axon segments of each group. (D) Confocal micrographs of DRG axons transfected with mCh-Caly-WT or mCh-Caly-ATEA (red) and stained with DIC (green) and AP-3 (blue). Manders coefficient of overlap was determined for colocalization of Caly-WT or Caly-ATEA with AP-3 in DIC positive puncta. Caly-WT exhibited greater colocalization with AP-3/DIC positive puncta than Caly-ATEA. Box-and-whisker plots show the Manders's tM2 of overlapping red and blue puncta in 100-mum axon segments of each group. Data plotted in histograms or in box-and-whisker plots correspond to results obtained in three independent experiments from at least five axons per experiment for each group; ** p < 0.01, *** p < 0.001; bar = 20 mum.
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- Invitrogen Antibodies (provider)
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- FIGURE 5: Caly stimulated dynein recruitment requires interaction with adaptor protein complexes. (A) Dynein labeling of LE/LROs in DRG axons transfected with GFP-Caly RNAi, GFP-Scr RNAi, mCherry vector, mCh-Caly-WT, or mCh-Caly-ATEA and stained with LAMP1 and PI4KIIalpha antibodies. Axons are outlined in white; filled arrows point to overlapping puncta, whereas hollow arrows show the examples of nonoverlapping puncta. (B) Greater dynein colocalization with LAMP1- or PI4KIIalpha-positive organelles is detected in Caly-WT-transfected axons, whereas less is observed in Caly RNAi-transfected axons. Data plotted in bar graphs correspond to results obtained in three independent experiments from at least five axons per experiment for each group; *** p < 0.001; bar = 10 mum.
