MA5-13259

antibody from Invitrogen Antibodies

Targeting: DES CMD1I, CSM1, CSM2

Provider product page for MA5-13259

Western blot

Immunocytochemistry

Immunohistochemistry

Other assay

Antibody data

Antibody Data
Antigen structure
References [80]
Comments [0]
Validations
Western blot [1]
Immunocytochemistry [2]
Immunohistochemistry [5]
Other assay [10]

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Product number: MA5-13259 - Provider product page
Provider: Invitrogen Antibodies
Product name: Desmin Monoclonal Antibody (D33)
Antibody type: Monoclonal
Antigen: Other
Description: MA5-13259 targets Desmin in IF and IHC (P) applications and shows reactivity with Bovine, Canine, Chicken, Feline, Hamster, Human, Non-human primate, and Rat samples.
Antibody clone number: D33
Concentration: Conc. Not Determined

DES protein structure - MA5-13259 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of desmin was performed using 70% confluent log phase RD cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Desmin Monoclonal Antibody (D33) (Product # MA5-13259) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32766, 1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoskeletal localization. Panel e represents MCF7 cells with no expression of Desmin. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunofluorescent analysis of Desmin (green) showing staining in the cytoplasm of human skeletal muscle (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Desmin monoclonal antibody (Product # MA5-13259) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Formalin-fixed, paraffin-embedded human leiomyoma stained with Desmin antibody using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of muscle cells.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Formalin-fixed, paraffin-embedded rat bladder stained with Desmin antibody using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of muscle cells.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical analysis of desmin was performed using formalin-fixed paraffin-embedded human colon adenocarcinoma tissue sections. To expose the target protein, heat-induced epitope retrieval was performed on de-paraffinized sections using eBioscience™ IHC Antigen Retrieval Solution - Low pH (10X) (Product # 00-4955-58) diluted to 1X solution in water in a decloaking chamber at 110 degree Celsius for 15 minutes. Following antigen retrieval, the sections were blocked with 3% H2O2 for 1 h at toom temperature followed by 2% normal goat serum in 1X PBS for 45 minutes at room temperature. The sections were then probed with or without Desmin Monoclonal Antibody (D33) (Product # MA5-13259) at 1:5000 dilution in 0.1% normal goat serum overnight at 4 degree Celsius in a humidified chamber. Detection was performed using Alexa Fluor™ 647 Tyramide SuperBoost™ Kit, goat anti-mouse IgG (Product # B40916). Nuclei were stained with DAPI (Product # D1306) and the sections were mounted using ProLong™ Glass Antifade Mountant (Product # P36984). The images were captured on EVOS™ M7000 Imaging System (Product # AMF7000) at 20X magnification and externally deconvoluted.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical analysis of desmin was performed using formalin-fixed paraffin-embedded human colon adenocarcinoma tissue sections. To expose the target protein, heat-induced epitope retrieval was performed on de-paraffinized sections using eBioscience™ IHC Antigen Retrieval Solution - Low pH (10X) (Product # 00-4955-58) diluted to 1X solution in water in a decloaking chamber at 110 degree Celsius for 15 minutes. Following antigen retrieval, the sections were blocked with 3% H2O2 for 1 h at toom temperature followed by 2% normal goat serum in 1X PBS for 45 minutes at room temperature. The sections were then probed with or without Desmin Monoclonal Antibody (D33) (Product # MA5-13259) at 1:5000 dilution in 0.1% normal goat serum overnight at 4 degree Celsius in a humidified chamber. Detection was performed using Alexa Fluor™ 647 Tyramide SuperBoost™ Kit, goat anti-mouse IgG (Product # B40916). Nuclei were stained with DAPI (Product # D1306) and the sections were mounted using ProLong™ Glass Antifade Mountant (Product # P36984). The images were captured on EVOS™ M7000 Imaging System (Product # AMF7000) at 20X magnification and externally deconvoluted.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical analysis of desmin was performed using formalin-fixed paraffin-embedded human colon adenocarcinoma tissue sections. To expose the target protein, heat-induced epitope retrieval was performed on de-paraffinized sections using eBioscience™ IHC Antigen Retrieval Solution - Low pH (10X) (Product # 00-4955-58) diluted to 1X solution in water in a decloaking chamber at 110 degree Celsius for 15 minutes. Following antigen retrieval, the sections were blocked with 3% H2O2 for 1 h at toom temperature followed by 2% normal goat serum in 1X PBS for 45 minutes at room temperature. The sections were then probed with or without Desmin Monoclonal Antibody (D33) (Product # MA5-13259) at 1:5000 dilution in 0.1% normal goat serum overnight at 4 degree Celsius in a humidified chamber. Detection was performed using Alexa Fluor™ 647 Tyramide SuperBoost™ Kit, goat anti-mouse IgG (Product # B40916). Nuclei were stained with DAPI (Product # D1306) and the sections were mounted using ProLong™ Glass Antifade Mountant (Product # P36984). The images were captured on EVOS™ M7000 Imaging System (Product # AMF7000) at 20X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Fig. 1 (A) Diffuse moderate immunolabeling for heat shock protein70 (HSP70) in cardiomyocytes of a control heart. (B) Dense immunolabeling of some foot-and-mouth disease virus (FMDV)-infected cardiomyocytes. Note the cytoplasmic and perinuclear (arrows). (C) Granularly sarcolemmal (arrowheads) and extracellular (arrows) localization of HSP70 immunolabeling. (D) alpha-BC immunolabeling in cross striations of cardiomyocytes in a control heart. (E) Abnormal accumulation of alpha-BC with coarse granules (arrows) in severely affected cardiomyocytes in a FMDV-infected heart. (F) Perinuclear localization of alpha-BC in the cross section of a FMDV-infected heart (arrows). (G) Desmin immunoreactivity in cross striations of cardiomyocytes from a control heart. (H) Abnormal distribution of desmin immunolabeling with a granular pattern in a FMDV-infected heart. Arrow indicates dense immunoreaction of Purkinje fibers. Immunohistochemical staining with streptavidin-biotin peroxidase complex (SABC) and 3-amino-9-ethylcarbazole (AEC), and counterstaining with Mayer's hematoxylin. A-D: x320, E and F: x160, G and H: x380.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: FIGURE 1 p16 expression is down-regulated during HSC activation. (A,B) C57/BL6 mice were induced to develop liver fibrosis by CCl 4 injection and primary HSCs were isolated as described in Methods. P16 expression was examined by qPCR and Western. (C,D) C57/BL6 mice were induced to develop liver fibrosis by BDL and primary HSCs were isolated as described in Methods. P16 expression was examined by qPCR and Western. (E,F) Primary mouse HSCs were isolated from C57/BL6 mice and allowed to undergo spontaneous activation. The cells were harvested at day 2 and day 8 and p16 expression was examined by qPCR and Western. (G,H) LX-2 cells were treated with or without TGF-beta (10 ng/mL) for 48 h. P16 expression was examined by qPCR and Western. Data represent averages of three independent experiments and error bars represent SEM. * p

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: FIGURE 4 EPA, DPA, and DHA protect against the inhibitory effects of PAL on myotube differentiation. Protein expression of the myogenic protein myogenin 120 h following treatment with 500 muM PAL and/or 50 muM EPA, DPA, or DHA, with a representative image of a stain free blot used for normalization shown with molecular weight markers indicated (A) . Quantified protein expression of the myogenic protein myogenin (B) . All densitometry values are expressed relative to protein content determined by stain free imaging and presented in arbitrary units relative to CON. Treatments that do not share letters are considered statistically significant ( p < 0.05; n = 6 biological replicates). Average number of myotubes/field (C) and fusion index (number of nuclei in tubes relative to the total number of nuclei expressed as a percentage) (D) . Representative images of each treatment group at 120 h ( n = 3 biological replicates) (E) . Scale bar represents 275 mum. Values are presented as mean +- SEM.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Figure 1 Ventricular myocardium irradiated with 30 Gy at 4 weeks. Irradiated left ventricular free wall subendocardium of rats was stained with hematoxylin and eosin (H&E), Masson trichrome (MT), and immunohistochemistry (Desmin, TUNEL [terminal deoxynucleotidal transferase-mediated biotin-deoxyuridine triphosphate nick-end labeling] assay, and C4d immunostaining). Myocyte necrosis and apoptosis were not observed in rat hearts irradiated by 20 to 50 Gy. Intercellular space (interstitium) was widened but not fibrotic. Cellular rupture and destruction of myocytes were not observed. (x400 Magnification.)

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Figure 4 Rdh10+/- (Het) males and females differ from each other and WT in muscle fiber characteristics. A , desmin immunostaining combined with DAPI staining of GM cryosections. The arrows denote centralized nuclei. Bars = 50 mum. Images were overexposed to allow visualization of nuclei. B , quantification of average number of fibers with centralized nuclei per field of view using H&E staining (averages of 3-48 sections from each of 3-4 mice), * p < 0.02. C , average CSA/mouse (1454-1635 individual fibers quantified for each sex and genotype). D and E , the ranges of the CSA frequency distribution of data in C .

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: 6 FIGURE Double whole-mount immunohistochemical stainings of the chicken thorax of HH-stage 24-30 using antibodies against Sox9 (green, left column) and Desmin (red, middle column) and overlays of both markers (right column). Dorsal is to the top and cranial is to the left, medial view. Sox9 is a marker of the chondrogenic lineage labelling the ribs, and Desmin is a marker of the myogenic lineage labelling musculature. Rib anlagen are numbered from Th1 to Th7. Arrowhead, intercostal muscle anlage. deg costal process and vertebral rib. + sternal rib. Abd, abdominal musculature before separation in anatomical muscle anlagen. Cs, costosternal muscle anlage; Iob, internal oblique abdominal muscle; Sm, putative anlage of the scalenus muscle; St, sternum; Tr, transverse abdominal muscle. Scale bar 500 mum.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: 7 FIGURE Close-ups showing the differentiation front of ribs and intercostal muscles in the ventrolateral body wall. Double whole-mount immunohistochemical stainings of the chicken thorax of HH-stages 25-28 using antibodies against Sox9 (green) and Desmin (red) in an overlay of both markers in the same embryo. Dorsal is to the top and cranial is to the left, medial view. The left column (a-d) shows the original stainings, and the right column (a' to d') shows the identical specimens, with the outline of the rib and intercostal muscle anlagen drawn in for clarity. Note that the muscle anlagen extend more ventrally than the rib anlagen (a-c), and reach the same ventral level only at HH-stage 28 when both anlagen have reached the sternum (d). Arrowhead, distal tip of intercostal muscle anlagen showing loose and variable muscle fibres. Arrow, muscle fibres extending into the domain of the prospective rib anlage. deg, costal process and vertebral rib anlage. +, sternal rib anlage. St, sternal anlage. Scale bar 100 mum.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Impaired development of embryonic B cell bursa follicle. (A) Histological images of WT, RAG1 +/- , and RAG1 -/- E18 bursa. The number of follicles (B) and diameter (C) of bursa follicles within 1-fold of bursa. The significance of differences among groups was calculated by one-way ANOVA; ns, no significance, ** P < 0.01 and **** P < 0.0001. (D) Embryonic bursa sections were stained with desmin at E18 to identify bursa follicle compartmentalization.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: 4 Figure Proliferation and inflammation of mesangial cells alleviated by cMet Ab treatment. A, Observation of cMet and desmin (a mesangial cells marker) expressions which are confirmed to merge well at the same site. Magnification: 800x (bar = 50 mum). B, Ki-67-positive cell expression and proliferation were increased after TNF-alpha stimulation and decreased dose-dependently after cMet Ab treatment. The data shown are representative of three independent experiments (N = 3/group; N = 5/group). Magnification: 400x (bar = 100 mum). C, Flow cytometry showed that the IL-8 + cells increased by approximately 1.7 times by TNF-alpha stimulation compared to the control. A dose-dependent decrease was observed on treatment with cMet Ab (N = 3/group). D, ICAM-1 expression was also increased on TNF-alpha stimulation; it was seen to reduce after cMet Ab treatment. Magnification: 800x (bar = 50 mum). The data shown are representative of three independent experiments. All data are presented as the mean +- SEM. * P < .05 (unpaired t test); ** P < .01 (unpaired t test); *** P < .001 (unpaired t test)