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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
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- Product number
- LS-C312974 - Provider product page
- Provider
- LSBio
- Product name
- NME1 / NM23 Antibody (aa137-152) LS-C312974
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Storage
- At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
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Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- WB of NME1 / NM23 antibody. All lanes: Anti-NME1 at 0.5ug/ml. Lane 1: Rat Heart Tissue Lysate at 40ug. Lane 2: Rat Brain Tissue Lysate at 40ug. Lane 3: Rat Liver Tissue Lysate at 40ug. Lane 4: Rat Skeletal Muscle Tissue Lysate at 40ug. Lane 5: PANC Whole Cell Lysate at 40ug. Lane 6: HELA Whole Cell Lysate at 40ug. Lane 7: SKOV Whole Cell Lysate at 40ug. Lane 8: COLO320 Whole Cell Lysate at 40ug. Predicted bind size: 17KD. Observed bind size: 17KD.
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- NME1 / NM23 antibody. ICC: HELA Cell.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- NME1 / NM23 antibody. ICC: HELA Cell.
Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- NME1 / NM23 antibody. IHC(P): Rat Cerebellum Tissue.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- IHC analysis of NM23A using anti-NM23A antibody. NM23A was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-NM23A Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
