MAB3336

antibody from Abnova Corporation

Targeting: GFAP FLJ45472

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB3336

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#MAB3336, RRID:AB_10551523

Product name

GFAP monoclonal antibody, clone SB61b

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against recombinant GFAP.

Isotype

IgG

Antibody clone number

SB61b

Storage

Store at 4°C.

References

Submitted references

Environmental enrichment rescues DYRK1A activity and hippocampal adult neurogenesis in TgDyrk1A.

Pons-Espinal M, Martinez de Lagran M, Dierssen M
Neurobiology of disease 2013 Dec;60:18-31

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Mutations in GFAP, encoding glial fibrillary acidic protein, are associated with Alexander disease.

Brenner M, Johnson AB, Boespflug-Tanguy O, Rodriguez D, Goldman JE, Messing A
Nature genetics 2001 Jan;27(1):117-20

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Expression of vimentin and glial fibrillary acidic protein in human developing spinal cord.

Lukás Z, Dráber P, Bucek J, Dráberová E, Viklický V, Stasková Z
The Histochemical journal 1989 Dec;21(12):693-701

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

The CCF-STTG1 cell line was fixed with 5% Acetic acid Methanol solution for 30 minutes followed by 5% Bovine serum PBS blocking , cells are permeabilized by 0.1% Triton X PBS for 15 mines before and stained with GFAP monoclonal antibody, clone SB61b (Cat # MAB3336) and then cells were fixed. (The cell washing buffer was 0.15% tween 20 in PBS.) Finally the slide was mounted with Fluormount-G contained DAPI.

Validation comment

Immunofluorescence