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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [1]
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- Product number
- MAB5897 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB5897, RRID:AB_10549810
- Product name
- GFAP monoclonal antibody, clone SB61b (HRP)
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against recombinant GFAP.
- Conjugate
- Horseradish Peroxidase
- Isotype
- IgG
- Antibody clone number
- SB61b
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Expression of vimentin and glial fibrillary acidic protein in human developing spinal cord.
Lukás Z, Dráber P, Bucek J, Dráberová E, Viklický V, Stasková Z
The Histochemical journal 1989 Dec;21(12):693-701
The Histochemical journal 1989 Dec;21(12):693-701
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Supportive validation
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- Abnova Corporation (provider)
- Main image
- Experimental details
- The CCF-STTG1 cell line was fixed with 5% acetic acid methanol solution for 30 minutes followed by 5% bovine serum PBS blocking. Cells were permeabilized by 0.1% Triton X PBS for 15 minutes prior to staining with GFAP monoclonal antibody, clone SB61b (CY3) (Cat # MAB5899). Cells were then fixed and the slide was mounted with DAPI-Fluormount-G.
- Validation comment
- Immunofluorescence
