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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [1]
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- Product number
- MAB5899 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB5899, RRID:AB_10550637
- Product name
- GFAP monoclonal antibody, clone SB61b (Cy3)
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against recombinant GFAP.
- Conjugate
- Yellow dye
- Isotype
- IgG
- Antibody clone number
- SB61b
- Storage
- Store at 4°C.
Submitted references Expression of vimentin and glial fibrillary acidic protein in human developing spinal cord.
Lukás Z, Dráber P, Bucek J, Dráberová E, Viklický V, Stasková Z
The Histochemical journal 1989 Dec;21(12):693-701
The Histochemical journal 1989 Dec;21(12):693-701
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Supportive validation
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- Abnova Corporation (provider)
- Main image
- Experimental details
- The CCF-STTG1 cell line was fixed with 5% acetic acid methanol solution for 30 minutes followed by 5% bovine serum PBS blocking. Cells were permeabilized by 0.1% Triton X PBS for 15 minutes prior to staining with GFAP monoclonal antibody, clone SB61b (CY3) (Cat # MAB5899). Cells were then fixed and the slide was mounted with DAPI-Fluormount-G.
- Validation comment
- Immunofluorescence
