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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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- Product number
- TA500337 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA500337, RRID:AB_2109821
- Product name
- GFAP mouse monoclonal antibody, clone OTI4G8 (formerly 4G8)
- Antibody type
- Monoclonal
- Description
- GFAP mouse monoclonal antibody, clone OTI4G8 (formerly 4G8)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- GFAP
- Isotype
- IgG
- Antibody clone number
- OTI4G8
- Vial size
- 100 µl
- Concentration
- 1.00mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-GFAP monoclonal antibody.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GFAP (RC204548, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GFAP.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- GFAP ELISA with 4G8 Capture (TA500337) and 2F7 Detection (TA700040) Antibodies. Substrate used: Recombinant Human GFAP (TP304548)
- Validation comment
- ELISA
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-GFAP mouse monoclonal antibody (TA500337) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GFAP(RC204548).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-GFAP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500337)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human embryonic brain cortex tissue within the normal limits using anti-GFAP mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120 oC for 3 min, TA500337)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human embryonic cerebellum within the normal limits using anti-GFAP mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3 min)(1:500)
- Validation comment
- IHC
