TA500341

antibody from OriGene

Targeting: GFAP FLJ45472

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA500341

Provider

OriGene

Proper citation

OriGene Cat#TA500341, RRID:AB_2055591

Product name

GFAP mouse monoclonal antibody, clone OTI2D8 (formerly 2D8)

Antibody type

Monoclonal

Description

GFAP mouse monoclonal antibody, clone OTI2D8 (formerly 2D8)

Host

Mouse

Conjugate

Unconjugated

Epitope

GFAP

Isotype

IgG

Antibody clone number

OTI2D8

Vial size

100 µl

Concentration

1.5 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-GFAP monoclonal antibody.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GFAP (RC204548, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GFAP.

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunofluorescent staining of A549 cells using anti-GFAP mouse monoclonal antibody (TA500341).

Validation comment

IF

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-GFAP mouse monoclonal antibody (TA500341) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GFAP(RC204548).

Validation comment

IF