TA500343

antibody from OriGene

Targeting: GFAP FLJ45472

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA500343

Provider

OriGene

Proper citation

OriGene Cat#TA500343, RRID:AB_2109653

Product name

GFAP mouse monoclonal antibody, clone OTI1E11 (formerly 1E11)

Antibody type

Monoclonal

Description

GFAP mouse monoclonal antibody, clone OTI1E11 (formerly 1E11)

Host

Mouse

Conjugate

Unconjugated

Epitope

GFAP

Isotype

IgG

Antibody clone number

OTI1E11

Vial size

100 µl

Concentration

1.00mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GFAP (RC204548, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GFAP.

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-GFAP mouse monoclonal antibody (TA500343) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GFAP(RC204548).

Validation comment

IF

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human embryonic brain cortex tissue within the normal limits using anti-GFAP mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120 oC for 3 min, TA500343)(1:500)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human embryonic cerebellum within the normal limits using anti-GFAP mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120 oC for 3 min, TA500343)(1:500)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded mouse and rat brain sections within the normal limits using anti-GFAP mouse monoclonal antibody.(1:500)

Validation comment

IHC