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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- 644701 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#644701, RRID:AB_2109791
- Product name
- Purified anti-GFAP
- Antibody type
- Monoclonal
- Antigen
- Bovine spinal cord homogenate
- Description
- GFAP is expressed exclusively in astrocytes in the central nervous system. The protein is a member of the intermediate filament family of proteins which form networks providing support and strength to cells. This antibody does not cross-react with other intermediate filaments such as vimentin, neurofilament proteins, desmin and others. More than 50 GFAP mutations were identifiled to associate with Alexander disease.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 25 µg
- Concentration
- 0.5 mg/ml
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- Mouse Brain extract cell lysates was resolved by electrophoresis, transferred to nitrocellulose and probed with purified 2E1.E9. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence system.
- Conjugate
- Unconjugated
