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Western blot
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- MA1-35376 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-GFAP Monoclonal Antibody (GFA-02)
- Antibody type
- Monoclonal
- Antigen
- Other
- Reactivity
- Human, Porcine
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- GFA-02
- Vial size
- 100 µg
- Concentration
- 0.1 mg/mL
- Storage
- 4° C
Submitted references Epigenetic regulation by chromatin activation mark H3K4me3 in primate progenitor cells within adult neurogenic niche.
Sandstrom RS, Foret MR, Grow DA, Haugen E, Rhodes CT, Cardona AE, Phelix CF, Wang Y, Berger MS, Lin CH
Scientific reports 2014 Jun 20;4:5371
Scientific reports 2014 Jun 20;4:5371
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-GFAP Monoclonal Antibody (GFA-02) (Product # MA1-35376) and a 50 kDa band corresponding to GFAP was observed across tissues tested except Mouse and Rat liver. Tissue extracts (30 µg lysate) of Mouse brain (Lane 1), Rat brain (Lane 2), Mouse liver (Lane 3) and Rat liver (Lane 4) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.1 ug/ml) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of GFAP using a monoclonal antibody (Product # MA1-35376).
