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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
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- Product number
- MA5-16367 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-GFAP Monoclonal Antibody (SP78)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is astrocytoma.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- SP78
- Vial size
- 500 µL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Effect of experimentally induced hypertension on cerebellum of postmenopausal rat.
El-Tahawy NFG, Abdel Hafez SMN, Ramzy MM, Zenhom NM, Abdel-Hamid HA
Journal of cellular physiology 2019 Aug;234(8):12941-12955
Journal of cellular physiology 2019 Aug;234(8):12941-12955
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot was performed using Anti-GFAP Monoclonal Antibody (SP78) (Product # MA5-16367) and a 50 kDa band corresponding to GFAP was observed across tissues tested except Mouse and Rat liver. Whole cell extracts (30 µg lysate) of Rat brain (Lane 1), Mouse liver (Lane 2) and Rat liver (Lane 3) in Fig. a and Mouse brain in Fig. b were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005) for Fig. a and SuperSignal™ West Dura Extended Duration Substrate (Product # 34076) for Fig. b.
