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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [26]
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- Validations
- Western blot [4]
- Immunohistochemistry [4]
- Flow cytometry [2]
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- Product number
- NB100-74340 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-74340, RRID:AB_1048019
- Product name
- Mouse Monoclonal Actin Antibody
- Antibody type
- Monoclonal
- Description
- IgM purified. Reacts with Actin 1, 2, 3, 4, 7, 8, 11 and 12.
- Reactivity
- Human, Mouse, Rat, Bovine, Drosophila, Rabbit, Sheep, Xenopus, Yeast, Zebrafish
- Host
- Mouse
- Isotype
- IgM
- Vial size
- 0.1 ml
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Mitochondrial glucose 6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase abrogate p53 induced apoptosis in a yeast model: Possible implications for apoptosis resistance in cancer cells.
Yeast Dop1 is required for glycosyltransferase retrieval from the trans-Golgi network.
The MicroRNA, miR-18a, Regulates NeuroD and Photoreceptor Differentiation in the Retina of Zebrafish.
FOXO3 directly regulates an autophagy network to functionally regulate proteostasis in adult neural stem cells.
Iron-deficiency anemia reduces cardiac contraction by downregulating RyR2 channels and suppressing SERCA pump activity.
Evaluation of Oral Antiretroviral Drugs in Mice With Metabolic and Neurologic Complications.
Direct repression of IGF2 is implicated in the anti-angiogenic function of microRNA-210 in human retinal endothelial cells.
Calorie restriction promotes cardiolipin biosynthesis and distribution between mitochondrial membranes.
Ginsenoside Rd attenuates breast cancer metastasis implicating derepressing microRNA-18a-regulated Smad2 expression.
PPFIA1 drives active α5β1 integrin recycling and controls fibronectin fibrillogenesis and vascular morphogenesis.
Panax notoginseng saponins attenuate lung cancer growth in part through modulating the level of Met/miR-222 axis.
Changes in interconnected pathways implicating microRNAs are associated with the activity of apocynin in attenuating myocardial fibrogenesis.
Chinese Herbal Medicine Treatment Improves the Overall Survival Rate of Individuals with Hypertension among Type 2 Diabetes Patients and Modulates In Vitro Smooth Muscle Cell Contractility.
Aloe-emodin exerts a potent anticancer and immunomodulatory activity on BRAF-mutated human melanoma cells.
Regulatory mechanism of human vascular smooth muscle cell phenotypic transformation induced by NELIN.
Integration of the catalytic subunit activates deneddylase activity in vivo as final step in fungal COP9 signalosome assembly.
Identification and characterization of a drug-sensitive strain enables puromycin-based translational assays in Saccharomyces cerevisiae.
The filamentous ascomycete Sordaria macrospora can survive in ambient air without carbonic anhydrases.
Control of multicellular development by the physically interacting deneddylases DEN1/DenA and COP9 signalosome.
Autophagy genes Smatg8 and Smatg4 are required for fruiting-body development, vegetative growth and ascospore germination in the filamentous ascomycete Sordaria macrospora.
Pathogenic prions deviate PrP(C) signaling in neuronal cells and impair A-beta clearance.
An allelic mutant series of ATM3 reveals its key role in the biogenesis of cytosolic iron-sulfur proteins in Arabidopsis.
The essential cytosolic iron-sulfur protein Nbp35 acts without Cfd1 partner in the green lineage.
Loss of BCL-XL in rod photoreceptors: Increased susceptibility to bright light stress.
The late pollen-specific actins in angiosperms.
The late pollen-specific actins in angiosperms.
Redhu AK, Bhat JP
Biochimica et biophysica acta. General subjects 2020 Mar;1864(3):129504
Biochimica et biophysica acta. General subjects 2020 Mar;1864(3):129504
Yeast Dop1 is required for glycosyltransferase retrieval from the trans-Golgi network.
Zhao SB, Suda Y, Nakanishi H, Wang N, Yoko-O T, Gao XD, Fujita M
Biochimica et biophysica acta. General subjects 2019 Jun;1863(6):1147-1157
Biochimica et biophysica acta. General subjects 2019 Jun;1863(6):1147-1157
The MicroRNA, miR-18a, Regulates NeuroD and Photoreceptor Differentiation in the Retina of Zebrafish.
Taylor SM, Giuffre E, Moseley P, Hitchcock PF
Developmental neurobiology 2019 Feb;79(2):202-219
Developmental neurobiology 2019 Feb;79(2):202-219
FOXO3 directly regulates an autophagy network to functionally regulate proteostasis in adult neural stem cells.
Audesse AJ, Dhakal S, Hassell LA, Gardell Z, Nemtsova Y, Webb AE
PLoS genetics 2019 Apr;15(4):e1008097
PLoS genetics 2019 Apr;15(4):e1008097
Iron-deficiency anemia reduces cardiac contraction by downregulating RyR2 channels and suppressing SERCA pump activity.
Chung YJ, Luo A, Park KC, Loonat AA, Lakhal-Littleton S, Robbins PA, Swietach P
JCI insight 2019 Apr 4;4(7)
JCI insight 2019 Apr 4;4(7)
Evaluation of Oral Antiretroviral Drugs in Mice With Metabolic and Neurologic Complications.
Tsai FJ, Ho MW, Lai CH, Chou CH, Li JP, Cheng CF, Wu YC, Liu X, Tsang H, Lin TH, Liao CC, Huang SM, Lin JC, Lin CC, Hsieh CL, Liang WM, Lin YJ
Frontiers in pharmacology 2018;9:1004
Frontiers in pharmacology 2018;9:1004
Direct repression of IGF2 is implicated in the anti-angiogenic function of microRNA-210 in human retinal endothelial cells.
Yang Q, Wang P, Du X, Wang W, Zhang T, Chen Y
Angiogenesis 2018 May;21(2):313-323
Angiogenesis 2018 May;21(2):313-323
Calorie restriction promotes cardiolipin biosynthesis and distribution between mitochondrial membranes.
Luévano-Martínez LA, Forni MF, Peloggia J, Watanabe IS, Kowaltowski AJ
Mechanisms of ageing and development 2017 Mar;162:9-17
Mechanisms of ageing and development 2017 Mar;162:9-17
Ginsenoside Rd attenuates breast cancer metastasis implicating derepressing microRNA-18a-regulated Smad2 expression.
Wang P, Du X, Xiong M, Cui J, Yang Q, Wang W, Chen Y, Zhang T
Scientific reports 2016 Sep 19;6:33709
Scientific reports 2016 Sep 19;6:33709
PPFIA1 drives active α5β1 integrin recycling and controls fibronectin fibrillogenesis and vascular morphogenesis.
Mana G, Clapero F, Panieri E, Panero V, Böttcher RT, Tseng HY, Saltarin F, Astanina E, Wolanska KI, Morgan MR, Humphries MJ, Santoro MM, Serini G, Valdembri D
Nature communications 2016 Nov 23;7:13546
Nature communications 2016 Nov 23;7:13546
Panax notoginseng saponins attenuate lung cancer growth in part through modulating the level of Met/miR-222 axis.
Yang Q, Wang P, Cui J, Wang W, Chen Y, Zhang T
Journal of ethnopharmacology 2016 Dec 4;193:255-265
Journal of ethnopharmacology 2016 Dec 4;193:255-265
Changes in interconnected pathways implicating microRNAs are associated with the activity of apocynin in attenuating myocardial fibrogenesis.
Yang Q, Cui J, Wang P, Du X, Wang W, Zhang T, Chen Y
European journal of pharmacology 2016 Aug 5;784:22-32
European journal of pharmacology 2016 Aug 5;784:22-32
Chinese Herbal Medicine Treatment Improves the Overall Survival Rate of Individuals with Hypertension among Type 2 Diabetes Patients and Modulates In Vitro Smooth Muscle Cell Contractility.
Lin YJ, Ho TJ, Yeh YC, Cheng CF, Shiao YT, Wang CB, Chien WK, Chen JH, Liu X, Tsang H, Lin TH, Liao CC, Huang SM, Li JP, Lin CW, Pang HY, Lin JG, Lan YC, Liu YH, Chen SY, Tsai FJ, Liang WM
PloS one 2015;10(12):e0145109
PloS one 2015;10(12):e0145109
Aloe-emodin exerts a potent anticancer and immunomodulatory activity on BRAF-mutated human melanoma cells.
Tabolacci C, Cordella M, Turcano L, Rossi S, Lentini A, Mariotti S, Nisini R, Sette G, Eramo A, Piredda L, De Maria R, Facchiano F, Beninati S
European journal of pharmacology 2015 Sep 5;762:283-92
European journal of pharmacology 2015 Sep 5;762:283-92
Regulatory mechanism of human vascular smooth muscle cell phenotypic transformation induced by NELIN.
Pei C, Qin S, Wang M, Zhang S
Molecular medicine reports 2015 Nov;12(5):7310-6
Molecular medicine reports 2015 Nov;12(5):7310-6
Integration of the catalytic subunit activates deneddylase activity in vivo as final step in fungal COP9 signalosome assembly.
Beckmann EA, Köhler AM, Meister C, Christmann M, Draht OW, Rakebrandt N, Valerius O, Braus GH
Molecular microbiology 2015 Jul;97(1):110-24
Molecular microbiology 2015 Jul;97(1):110-24
Identification and characterization of a drug-sensitive strain enables puromycin-based translational assays in Saccharomyces cerevisiae.
Cary GA, Yoon SH, Torres CG, Wang K, Hays M, Ludlow C, Goodlett DR, Dudley AM
Yeast (Chichester, England) 2014 May;31(5):167-78
Yeast (Chichester, England) 2014 May;31(5):167-78
The filamentous ascomycete Sordaria macrospora can survive in ambient air without carbonic anhydrases.
Lehneck R, Elleuche S, Pöggeler S
Molecular microbiology 2014 Jun;92(5):931-44
Molecular microbiology 2014 Jun;92(5):931-44
Control of multicellular development by the physically interacting deneddylases DEN1/DenA and COP9 signalosome.
Christmann M, Schmaler T, Gordon C, Huang X, Bayram O, Schinke J, Stumpf S, Dubiel W, Braus GH
PLoS genetics 2013;9(2):e1003275
PLoS genetics 2013;9(2):e1003275
Autophagy genes Smatg8 and Smatg4 are required for fruiting-body development, vegetative growth and ascospore germination in the filamentous ascomycete Sordaria macrospora.
Voigt O, Pöggeler S
Autophagy 2013 Jan;9(1):33-49
Autophagy 2013 Jan;9(1):33-49
Pathogenic prions deviate PrP(C) signaling in neuronal cells and impair A-beta clearance.
Pradines E, Hernandez-Rapp J, Villa-Diaz A, Dakowski C, Ardila-Osorio H, Haik S, Schneider B, Launay JM, Kellermann O, Torres JM, Mouillet-Richard S
Cell death & disease 2013 Jan 10;4:e456
Cell death & disease 2013 Jan 10;4:e456
An allelic mutant series of ATM3 reveals its key role in the biogenesis of cytosolic iron-sulfur proteins in Arabidopsis.
Bernard DG, Cheng Y, Zhao Y, Balk J
Plant physiology 2009 Oct;151(2):590-602
Plant physiology 2009 Oct;151(2):590-602
The essential cytosolic iron-sulfur protein Nbp35 acts without Cfd1 partner in the green lineage.
Bych K, Netz DJ, Vigani G, Bill E, Lill R, Pierik AJ, Balk J
The Journal of biological chemistry 2008 Dec 19;283(51):35797-804
The Journal of biological chemistry 2008 Dec 19;283(51):35797-804
Loss of BCL-XL in rod photoreceptors: Increased susceptibility to bright light stress.
Zheng L, Anderson RE, Agbaga MP, Rucker EB 3rd, Le YZ
Investigative ophthalmology & visual science 2006 Dec;47(12):5583-9
Investigative ophthalmology & visual science 2006 Dec;47(12):5583-9
The late pollen-specific actins in angiosperms.
Kandasamy MK, McKinney EC, Meagher RB
The Plant journal : for cell and molecular biology 1999 Jun;18(6):681-91
The Plant journal : for cell and molecular biology 1999 Jun;18(6):681-91
The late pollen-specific actins in angiosperms.
Kandasamy MK, McKinney EC, Meagher RB
The Plant journal : for cell and molecular biology 1999 Jun;18(6):681-91
The Plant journal : for cell and molecular biology 1999 Jun;18(6):681-91
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Actin Antibody (mAbGEa) [NB100-74340] - Analysis of Actin expression in 2) HeLa, 3) NTERA-2, 4) A431, 5) HepG2, 6) MCF7, 7) NIH-3T3, 8) PC-12 and 9) COS-7 whole cell lysates.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Actin Antibody (mAbGEa) [NB100-74340] - Analysis of Jurkat and CHO cell lysates using actin antibody [NB100-74340] at 1:100.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: Actin Antibody (mAbGEa) [NB100-74340] - Image shows a specific band for Actin in 0.1 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Actin Antibody (mAbGEa) [NB100-74340] - Total protein from HeLa, 3T3, PC12 and Bovine normal tissue was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/mL anti-Actin in 5% blocking buffer and detected with an anti-mouse IgM secondary antibody using chemiluminescence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Actin Antibody (mAbGEa) [NB100-74340] - Analysis of Actin on human breast cancer tissue using DAB with hematoxylin counterstain.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Actin Antibody (mAbGEa) [NB100-74340] - Both normal and cancer biopsies of deparaffinized Human skeletal muscle tissues.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Actin Antibody (mAbGEa) [NB100-74340] - Both normal and cancer biopsies of deparaffinized Human colon carcinoma tissues.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Actin Antibody (mAbGEa) [NB100-74340] - Both normal and cancer biopsies of deparaffinized Human tonsil tissues.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Actin Antibody (mAbGEa) [NB100-74340] - Analysis of HeLa cells using mouse Actin antibody (Orange) and Isotype control Antibody (Blue).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow (Intracellular): Actin Antibody (mAbGEa) [NB100-74340] - An intracellular stain was performed on A549 cells with NB100-74340 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by mouse IgM Alexa Fluor 488-conjugated secondary antibody.
