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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [12]
- Comments [0]
- Validations
- Western blot [4]
- Immunoprecipitation [1]
- Immunohistochemistry [3]
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- Product number
- NB600-535 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB600-535, RRID:AB_2222878
- Product name
- Mouse Monoclonal pan Actin Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. This antibody reacts with all six known vertebrate isoforms of actin (approximately 42kDa). It also reacts with two cytoplasmic actins (Beta, gamma) which are highly homologous to one another but which differ from the muscle actins at about 25 amino acid residues.
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Porcine, Rabbit
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 500 uL
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Nanobodies reveal an extra-synaptic population of SNAP-25 and Syntaxin 1A in hippocampal neurons.
Comparative synaptosome imaging: a semi-quantitative method to obtain copy numbers for synaptic and neuronal proteins.
An Alzheimer's Disease-Linked Loss-of-Function CLN5 Variant Impairs Cathepsin D Maturation, Consistent with a Retromer Trafficking Defect.
Calreticulin attenuated microwave radiation-induced human microvascular endothelial cell injury through promoting actin acetylation and polymerization.
Disruption of amyloid precursor protein ubiquitination selectively increases amyloid β (Aβ) 40 levels via presenilin 2-mediated cleavage.
Exercise-induced mitochondrial p53 repairs mtDNA mutations in mutator mice.
Oncogenic BRAF(V600E) Induces Clastogenesis and UVB Hypersensitivity.
Diacylglycerol kinase α regulates tubular recycling endosome biogenesis and major histocompatibility complex class I recycling.
Role of phosphatidylinositol 4,5-bisphosphate in regulating EHD2 plasma membrane localization.
PDK1 decreases TACE-mediated α-secretase activity and promotes disease progression in prion and Alzheimer's diseases.
Plectin 1 links intermediate filaments to costameric sarcolemma through beta-synemin, alpha-dystrobrevin and actin.
Two monoclonal antibodies to actin: one muscle selective and one generally reactive.
Maidorn M, Olichon A, Rizzoli SO, Opazo F
mAbs 2019 Feb Mar;11(2):305-321
mAbs 2019 Feb Mar;11(2):305-321
Comparative synaptosome imaging: a semi-quantitative method to obtain copy numbers for synaptic and neuronal proteins.
Richter KN, Wildhagen H, Helm MS, Ußling JE, Schikorski T, Rizzoli SO
Scientific reports 2018 Oct 4;8(1):14838
Scientific reports 2018 Oct 4;8(1):14838
An Alzheimer's Disease-Linked Loss-of-Function CLN5 Variant Impairs Cathepsin D Maturation, Consistent with a Retromer Trafficking Defect.
Qureshi YH, Patel VM, Berman DE, Kothiya MJ, Neufeld JL, Vardarajan B, Tang M, Reyes-Dumeyer D, Lantigua R, Medrano M, Jiménez-Velázquez IJ, Small SA, Reitz C
Molecular and cellular biology 2018 Oct 15;38(20)
Molecular and cellular biology 2018 Oct 15;38(20)
Calreticulin attenuated microwave radiation-induced human microvascular endothelial cell injury through promoting actin acetylation and polymerization.
Xu F, Wang Y, Tao T, Song D, Liu X
Cell stress & chaperones 2017 Jan;22(1):87-97
Cell stress & chaperones 2017 Jan;22(1):87-97
Disruption of amyloid precursor protein ubiquitination selectively increases amyloid β (Aβ) 40 levels via presenilin 2-mediated cleavage.
Williamson RL, Laulagnier K, Miranda AM, Fernandez MA, Wolfe MS, Sadoul R, Di Paolo G
The Journal of biological chemistry 2017 Dec 1;292(48):19873-19889
The Journal of biological chemistry 2017 Dec 1;292(48):19873-19889
Exercise-induced mitochondrial p53 repairs mtDNA mutations in mutator mice.
Safdar A, Khrapko K, Flynn JM, Saleem A, De Lisio M, Johnston AP, Kratysberg Y, Samjoo IA, Kitaoka Y, Ogborn DI, Little JP, Raha S, Parise G, Akhtar M, Hettinga BP, Rowe GC, Arany Z, Prolla TA, Tarnopolsky MA
Skeletal muscle 2016;6:7
Skeletal muscle 2016;6:7
Oncogenic BRAF(V600E) Induces Clastogenesis and UVB Hypersensitivity.
Simpson DA, Lemonie N, Morgan DS, Gaddameedhi S, Kaufmann WK
Cancers 2015 Jun 17;7(2):1072-90
Cancers 2015 Jun 17;7(2):1072-90
Diacylglycerol kinase α regulates tubular recycling endosome biogenesis and major histocompatibility complex class I recycling.
Xie S, Naslavsky N, Caplan S
The Journal of biological chemistry 2014 Nov 14;289(46):31914-26
The Journal of biological chemistry 2014 Nov 14;289(46):31914-26
Role of phosphatidylinositol 4,5-bisphosphate in regulating EHD2 plasma membrane localization.
Simone LC, Caplan S, Naslavsky N
PloS one 2013;8(9):e74519
PloS one 2013;8(9):e74519
PDK1 decreases TACE-mediated α-secretase activity and promotes disease progression in prion and Alzheimer's diseases.
Pietri M, Dakowski C, Hannaoui S, Alleaume-Butaux A, Hernandez-Rapp J, Ragagnin A, Mouillet-Richard S, Haik S, Bailly Y, Peyrin JM, Launay JM, Kellermann O, Schneider B
Nature medicine 2013 Sep;19(9):1124-31
Nature medicine 2013 Sep;19(9):1124-31
Plectin 1 links intermediate filaments to costameric sarcolemma through beta-synemin, alpha-dystrobrevin and actin.
Hijikata T, Nakamura A, Isokawa K, Imamura M, Yuasa K, Ishikawa R, Kohama K, Takeda S, Yorifuji H
Journal of cell science 2008 Jun 15;121(Pt 12):2062-74
Journal of cell science 2008 Jun 15;121(Pt 12):2062-74
Two monoclonal antibodies to actin: one muscle selective and one generally reactive.
Lessard JL
Cell motility and the cytoskeleton 1988;10(3):349-62
Cell motility and the cytoskeleton 1988;10(3):349-62
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: pan Actin Antibody (ACTN05 (C4)) [NB600-535] - Immunoprecipitation and Western Blot analaysis on pan Actin from Human Cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: pan Actin Antibody (ACTN05 (C4)) [NB600-535] - Analysis of Actin in MCF-7 and HCT116 cell lysates (20ug/lane). Image from verified customer review.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: pan Actin Antibody (ACTN05 (C4)) [NB600-535] - Analysis of 25ug of various whole cell lysates and 10ul of PageRuler Plus Prestained Protein Ladder per well onto a 4-20% Tris-HCl polyacrylamide gel.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: pan Actin Antibody (ACTN05 (C4)) [NB600-535] - Analysis of HeLa Cells.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: pan Actin Antibody (ACTN05 (C4)) [NB600-535] - Analysis of denatured Human HeLa Cells.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: pan Actin Antibody (ACTN05 (C4)) [NB600-535] - Analysis of Actin pan showing positive staining in the cytoplasm of Human prostate carcinoma (right) compared with a negative control in the absence of primary antibody (left).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: pan Actin Antibody (ACTN05 (C4)) [NB600-535] - Analysis showing positive staining in the cytoplasm of Human skeletal muscle (right) compared with a negative control in the absence of primary antibody (left).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: pan Actin Antibody (ACTN05 (C4)) [NB600-535] - Analysis showing positive staining in the cytoplasm of Mouse skeletal muscle (right) compared with a negative control in the absence of primary antibody (left).
