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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunohistochemistry [6]
- Flow cytometry [1]
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- Product number
- 17521-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ACTA1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is specific to the ACTA1. It does not cross-react with other actin isoforms.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.15 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17521-1-AP (ACTA1-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17521-1-AP (ACTA1-Specific Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17521-1-AP (ACTA1-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human skeletal muscle slide using 17521-1-AP (ACTA1-Specific Antibody) at dilution of 1:50.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human skeletal muscle slide using 17521-1-AP (ACTA1-Specific Antibody) at dilution of 1:50.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human hysteromyoma using 17521-1-AP (ACTA1-Specific antibody) at dilution of 1:50 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human hysteromyoma using 17521-1-AP (ACTA1-Specific antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human skeletal muscle tissue slide using 17521-1-AP ( ACTA1-Specific Antibody) at dilution of 1:500 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human skeletal muscle tissue slide using 17521-1-AP ( ACTA1-Specific Antibody) at dilution of 1:500 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 C2C12 cells were intracellularly stained with 0.4 µg Anti-Human ACTA1-Specific (Product # 17521-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1,000 (red), or 0.4 µg Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
