MA5-37536

antibody from Invitrogen Antibodies

Targeting: ACTA1 ACTA, NEM3

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-37536

Provider

Invitrogen Antibodies

Product name

ACTA1 Monoclonal Antibody (337CT30.10.1)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

337CT30.10.1

Vial size

400 µL

Concentration

0.5 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of ACTA1 using the ACTA1 Monoclonal Antibody (Product # MA5-37536) at a 1:500-1:1000 dilution. Lane 1: MDA-MB231 whole cell lysate, Lane 2: MDA-MB-453 whole cell lysate, Lane 3: Hela whole cell lysate, Lane 4: mouse liver lysate, Lane 5: CEM whole cell lysate. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1:10,000 dilution. Predicted band size : 42 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of ACTA1 using the ACTA1 Monoclonal Antibody (Product # MA5-37536) at a 1:1000 dilution. Lane 1: A431 whole cell lysate, Lane 2: Hela whole cell lysate. Lysates/proteins were loaded at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1:10,000 dilution. Predicted band size : 42 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of ACTA1 in formalin fixed and paraffin embedded human colon carcinoma using the ACTA1 Monoclonal Antibody (Product # MA5-37536) at a dilution of 1:10-1:50.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of ACTA1 in formalin fixed and paraffin embedded human skeletal muscle using the ACTA1 Monoclonal Antibody (Product # MA5-37536) at a dilution of 1:10-1:50, followed by peroxidase conjugation of the secondary antibody and DAB staining.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis performed on A549 cells (right histogram) compared to a negative control cell (left histogram) using ACTA1 Monoclonal Antibody (Product # MA5-37536) at a 1:10-1:50 dilution. FITC-conjugated goat-anti-mouse secondary antibodies were used for the analysis.