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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Flow cytometry [1]
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- Product number
- 17-5139-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD85k (ILT3) Monoclonal Antibody (ZM4.1), APC, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The monoclonal antibody ZM4.1 recognizes ILT3, also known as CD85k. ILT3 is a member of the large family of ILT molecules (Immunologulin-like Transcripts, Leucocyte Ig-like Receptors (LIRs) and Monocyte/Macrophage Ig-like Receptors (MIRs). ILT3 is a 60kDa transmembrane protein with two Ig domains and ITIMs. Expression is found on monocytes, macrophages, and dendritic cells. High levels of ILT3 expression is also found on plasmacytoid dendritic cells (pDCs). ILT3 expression by DCs has been shown to be required to induce Regulatory Tcells (tregs). Addition of ZM4.1, like ZM3.8, blocks the suppressive function of the Tregs in in vitro culture systems with allogenic DC's. Applications Reported: This ZM4.1 antibody has been reported for use in flow cytometric analysis. Applications Tested: This ZM4.1 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- ZM4.1
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Targeting FTO Suppresses Cancer Stem Cell Maintenance and Immune Evasion.
Expression of the inhibitory receptor ILT3 on dendritic cells is dispensable for induction of CD4+Foxp3+ regulatory T cells by 1,25-dihydroxyvitamin D3.
A novel inhibitory receptor (ILT3) expressed on monocytes, macrophages, and dendritic cells involved in antigen processing.
Su R, Dong L, Li Y, Gao M, Han L, Wunderlich M, Deng X, Li H, Huang Y, Gao L, Li C, Zhao Z, Robinson S, Tan B, Qing Y, Qin X, Prince E, Xie J, Qin H, Li W, Shen C, Sun J, Kulkarni P, Weng H, Huang H, Chen Z, Zhang B, Wu X, Olsen MJ, Müschen M, Marcucci G, Salgia R, Li L, Fathi AT, Li Z, Mulloy JC, Wei M, Horne D, Chen J
Cancer cell 2020 Jul 13;38(1):79-96.e11
Cancer cell 2020 Jul 13;38(1):79-96.e11
Expression of the inhibitory receptor ILT3 on dendritic cells is dispensable for induction of CD4+Foxp3+ regulatory T cells by 1,25-dihydroxyvitamin D3.
Penna G, Roncari A, Amuchastegui S, Daniel KC, Berti E, Colonna M, Adorini L
Blood 2005 Nov 15;106(10):3490-7
Blood 2005 Nov 15;106(10):3490-7
A novel inhibitory receptor (ILT3) expressed on monocytes, macrophages, and dendritic cells involved in antigen processing.
Cella M, Döhring C, Samaridis J, Dessing M, Brockhaus M, Lanzavecchia A, Colonna M
The Journal of experimental medicine 1997 May 19;185(10):1743-51
The Journal of experimental medicine 1997 May 19;185(10):1743-51
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Anti-Human CD123 FITC (Product # 11-1239-42) and Mouse IgG1 kappa Isotype Control APC (Product # 17-4714-81) (left) or Anti-Human CD85k (ILT3) APC (right). Cells in the monocyte gate were used for analysis.
