AM2022a

antibody from Abgent

Targeting: ALPP PALP, PLAP

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AM2022a

Provider

Abgent

Proper citation

Abgent Cat#AM2022a, RRID:AB_11137296

Product name

ALPP Antibody (N-term)(Ascites)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

Mouse Monoclonal Antibody (Mab)

Reactivity

Human

Host

Mouse

Isotype

IgG

Vial size

100 µl

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

ALPP Antibody (N-term) (Cat. #AM2022a) western blot analysis in mouse placenta tissue lysates (35ug/lane).This demonstrates the ALPP antibody detected the ALPP protein (arrow).

Primary Ab dilution

1:500~3200

Immunocytochemistry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

"Fluorescent confocal image of HeLa cells stained with ALPP (N-term) antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AM2022a ALPP (N-term) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ¼g/ml, 5 min). ALPP immunoreactivity is localized to the cytoplasm of HeLa cells. "

Primary Ab dilution

1:100