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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- H00000250-D01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00000250-D01, RRID:AB_10718575
- Product name
- ALPP MaxPab rabbit polyclonal antibody (D01)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against a full-length human ALPP protein.
- Antigen sequence
MLGPCMLLLLLLLGLRLQLSLGIIPVEEENPDFWN
REAAEALGAAKKLQPAQTAAKNLIIFLGDGMGVST
VTAARILKGQKKDKLGPEIPLAMDRFPYVALSKTY
NVDKHVPDSGATATAYLCGVKGNFQTIGLSAAARF
NQCNTTRGNEVISVMNRAKKAGKSVGVVTTTRVQH
ASPAGTYAHTVNRNWYSDADVPASARQEGCQDIAT
QLISNMDIDVILGGGRKYMFRMGTPDPEYPDDYSQ
GGTRLDGKNLVQEWLAKRQGARYVWNRTELMQASL
DPSVTHLMGLFEPGDMKYEIHRDSTLDPSLMEMTE
AALRLLSRNPRGFFLFVEGGRIDHGHHESRAYRAL
TETIMFDDAIERAGQLTSEEDTLSLVTADHSHVFS
FGGYPLRGSSIFGLAPGKARDRKAYTVLLYGNGPG
YVLKDGARPDVTESESGSPEYRQQSAVPLDEETHA
GEDVAVFARGPQAHLVHGVQEQTFIAHVMAFAACL
EPYTACDLAPPAGTTDAAHPGRSVVPALLPLLAGT
LLLLETATAP- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of ALPP expression in transfected 293T cell line (H00000250-T01) by ALPP MaxPab polyclonal antibody.Lane 1: ALPP transfected lysate(58 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation of ALPP transfected lysate using anti-ALPP MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with ALPP purified MaxPab mouse polyclonal antibody (B01P) (H00000250-B01P).
- Validation comment
- Immunoprecipitation
- Protocol
- Protocol
