MAB5905

antibody from R&D Systems

Targeting: ALPP PALP, PLAP

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Antibody Data

Product number

MAB5905

Provider

R&D Systems

Product name

Human Alkaline Phosphatase/ALPP Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human Alkaline Phosphatase/ALPP in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) Intestinal Alkaline Phosphatase/ALPI or rhAlkaline Phsophatase/ALPL is observed.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

P05187


Isotype

IgG

Antibody clone number

696108

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human Alkaline Phosphatase/ALPP by Western Blot. Western blot shows lysates of JAR human choriocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Alkaline Phosphatase/ALPP Monoclonal Antibody (Catalog # MAB5905) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Alkaline Phosphatase/ALPP at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.