42040

antibody from QED Bioscience

Targeting: FN1 CIG, FINC, GFND2, LETS, MSF

Western blot (Data presented on provider website)

ELISA (Data presented on provider website)

Blocking/Neutralizing (Data presented on provider website)

Antibody Data

Product number

42040

Provider

QED Bioscience

Product name

Anti-Fibronectin Antibody (42040)

Antibody type

Monoclonal

Description

Anti-Human Fibronectin Mouse Monoclonal Antibody

Reactivity

Human, Bovine

Host

Mouse

Epitope

Human plasma fibronectin

Isotype

IgG

Antibody clone number

FN12-8

Vial size

400ug

Concentration

Lot Specific

Storage

This antibody is stable for 1 month at 4°C or one year at -20°C. Avoid multiple freeze-thaw cycles.

Handling

Dissolve lyophilized antibody in 200ul of distilled water (final concentration 2.0mg/ml).ELISA: 1-10ug/ml with immobilized antigen. Western Blot: 1-10ug/ml, reducing or non-reducing conditions. Cell Adhesion Inhibition Assay: 0.1-1.0ug/ml on 10ug/ml fibronectin-coated microwells inhibits adhesion of trypsinized NRK cells. Immunohistochemistry: 1-10ug/ml, paraffin-embedded or frozen tissue sections with Proteinase K treatment for antigen retrieval. Staining of fibronectin (FN) in human well-differentiated colon cancer tissue (bottom) and moderately- differentiated colon cancer (top) with #42040. These are recommended concentrations. Endusers should determine optimal concentrations for their applications.