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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [6]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- MAB1918 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Fibronectin Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Fibronectin in Western blots.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- P1H11
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Diabetes Aggravates Post-ischaemic Renal Fibrosis through Persistent Activation of TGF-β1 and Shh Signalling.
PDL regeneration via cell homing in delayed replantation of avulsed teeth.
Granzyme B releases vascular endothelial growth factor from extracellular matrix and induces vascular permeability.
CD29 expressed on plasma cells is activated by divalent cations and soluble CD106 contained in the bone marrow plasma: refractory activation is associated with enhanced proliferation and exit of clonal plasma cells to circulation in multiple myeloma patients.
Snail involves in the transforming growth factor β1-mediated epithelial-mesenchymal transition of retinal pigment epithelial cells.
Characterization and functional analysis of osteoblast-derived fibulins in the human hematopoietic stem cell niche.
Kim DJ, Kang JM, Park SH, Kwon HK, Song SJ, Moon H, Kim SM, Seo JW, Lee YH, Kim YG, Moon JY, Lee SY, Son Y, Lee SH
Scientific reports 2017 Dec 1;7(1):16782
Scientific reports 2017 Dec 1;7(1):16782
PDL regeneration via cell homing in delayed replantation of avulsed teeth.
Zhu W, Zhang Q, Zhang Y, Cen L, Wang J
Journal of translational medicine 2015 Nov 14;13:357
Journal of translational medicine 2015 Nov 14;13:357
Granzyme B releases vascular endothelial growth factor from extracellular matrix and induces vascular permeability.
Hendel A, Hsu I, Granville DJ
Laboratory investigation; a journal of technical methods and pathology 2014 Jul;94(7):716-25
Laboratory investigation; a journal of technical methods and pathology 2014 Jul;94(7):716-25
CD29 expressed on plasma cells is activated by divalent cations and soluble CD106 contained in the bone marrow plasma: refractory activation is associated with enhanced proliferation and exit of clonal plasma cells to circulation in multiple myeloma patients.
Martínez-Viñambres E, García-Trujillo JA, Rodríguez-Martín E, Villar LM, Coll J, Roldán E
Leukemia 2012 May;26(5):1098-105
Leukemia 2012 May;26(5):1098-105
Snail involves in the transforming growth factor β1-mediated epithelial-mesenchymal transition of retinal pigment epithelial cells.
Li H, Wang H, Wang F, Gu Q, Xu X
PloS one 2011;6(8):e23322
PloS one 2011;6(8):e23322
Characterization and functional analysis of osteoblast-derived fibulins in the human hematopoietic stem cell niche.
Hergeth SP, Aicher WK, Essl M, Schreiber TD, Sasaki T, Klein G
Experimental hematology 2008 Aug;36(8):1022-34
Experimental hematology 2008 Aug;36(8):1022-34
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Fibronectin by Simple WesternTM. Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line and A549 human lung carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Fibronectin at approximately 285 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human Fibronectin Monoclonal Antibody (Catalog # MAB1918) . This experiment was conducted under reducing conditions and using the 66-440 kDa separation system.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Fibronectin in HepG2 and Daudi Human Cell Lines. Fibronectin was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line (positive staining; left panel) and Daudi human Burkitt's lymphoma cell line (negative staining; right panel) using Mouse Anti-Human Fibronectin Monoclonal Antibody (Catalog # MAB1918) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Fibronectin in Human Breast Cancer Tissue. Fibronectin was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-Human Fibronectin Monoclonal Antibody (Catalog # MAB1918) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to connective tissue and fibroblasts. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
