- MA1-46028 - Invitrogen Antibodies WT1 antibody

Submitted references Evaluation of the Preclinical Efficacy of Lurbinectedin in Malignant Pleural Mesothelioma.

Anobile DP, Bironzo P, Picca F, Lingua MF, Morena D, Righi L, Napoli F, Papotti MG, Pittaro A, Di Nicolantonio F, Gigliotti C, Bussolino F, Comunanza V, Guerrera F, Sandri A, Leo F, Libener R, Aviles P, Novello S, Taulli R, Scagliotti GV, Riganti C
Cancers 2021 May 12;13(10)

SeqStain is an efficient method for multiplexed, spatialomic profiling of human and murine tissues.

Rajagopalan A, Venkatesh I, Aslam R, Kirchenbuechler D, Khanna S, Cimbaluk D, Kordower JH, Gupta V
Cell reports methods 2021 Jun 21;1(2)

Evaluating the transcriptional fidelity of cancer models.

Peng D, Gleyzer R, Tai WH, Kumar P, Bian Q, Isaacs B, da Rocha EL, Cai S, DiNapoli K, Huang FW, Cahan P
Genome medicine 2021 Apr 29;13(1):73

Amniotic fluid stem cells ameliorate cisplatin-induced acute renal failure through induction of autophagy and inhibition of apoptosis.

Minocha E, Sinha RA, Jain M, Chaturvedi CP, Nityanand S
Stem cell research & therapy 2019 Dec 4;10(1):370

Identification of fatal outcome in a childhood nasopharyngeal carcinoma patient by protein expression profiling.

Saeed MEM, Mertens R, Handgretinger R, Efferth T
International journal of oncology 2018 Oct;53(4):1721-1731

Epicardial Outgrowth Culture Assay and Ex Vivo Assessment of Epicardial-derived Cell Migration.

Trembley MA, Velasquez LS, Small EM
Journal of visualized experiments : JoVE 2016 Mar 18;(109)

Wilms' tumor 1 protein expression in endometrial adenocarcinoma and endometrial intra-epithelial neoplasia.

Atik Y, Cetinkaya Demir B, Ozan H, Baykara S, Usubutun A, Yılmaz Erturk F
The journal of obstetrics and gynaecology research 2016 Jul;42(7):870-5

Expression of mesothelioma-related markers in meningiomas: an immunohistochemical study.

Abdelzaher E, Abdallah DM
BioMed research international 2014;2014:968794

HIF-1α, VEGF and WT-1 are protagonists in bilateral primary angiosarcoma of breast: a case report and review of literature.

Al-Salam S, Balalaa N, Faour I, Akhter S, Alashari M
International journal of clinical and experimental pathology 2012;5(3):247-53

Mixed epithelial and stromal tumor of the kidney in a 12-year-old girl.

Hara N, Kawaguchi M, Murayama S, Maruyama R, Tanikawa T, Takahashi K
Pathology international 2005 Oct;55(10):670-6

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot of WT1 in 293 cell lysate using Product # MA1-46028.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of WT1 in whole cell protein from human K562, HEK293 and kidney tissue. Samples were incubated in WT1 monoclonal antibody (Product # MA1-46028) using a dilution of 2 µg/mL followed by an anti-mouse HRP secondary antibody. Separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. Detection: chemiluminescence.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of WT1 in 0.5 mg/mL Hek293 lysate. Samples were incubated in WT1 monoclonal (Product # MA1-46028). This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical analysis of WT1 in human renal cancer. Samples were incubated with WT1 monoclonal antibody (Product # MA1-46028) followed by DAB with hematoxylin counterstain.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 3 Immunofluorescence of selected cell lines. a Classification profiles (left) and IF expression (middle) of Caov-4 (OV-positive control), HEC-59 (UCEC-positive control), and SK-OV-3 for WT1 (OV biomarker) and HOXB6 (uterine biomarker). The rightmost bar plots quantify the average percentage of positive cells for WT1 (top-right) and HOXB6 (bottom-right). b Classification profiles (left) and IF expression (middle) of Caov-4, NCCIT (germ cell tumor-positive control), and A2780 for WT1 and LIN28A (germ cell tumor biomarker). Classification was performed on replicates of NCCIT RNA-seq profiles. The rightmost bar plots quantify the average percentage of positive cells for WT1 (top-right) and LIN28A (bottom-right). c Classification profiles (left) and IF expression (middle) of Vcap (PRAD positive control), RT4 (BLCA-positive control), and PC-3 for FOLH1 (prostate biomarker) and PPARG (urothelial biomarker). The rightmost bar plots quantify the average percentage of positive cells for FOLH1 (top-right) and PPARG (bottom-right)

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 1 Characterization of patient-derived MPM cell lines. ( A ) Representative images showing different morphology of three BAP1 positive (BAP1+) and three BAP1 negative (BAP1-) MPM cell lines (scale bar = 100 um). ( B ) Flow cytometry plot representing the percentage of pancytokeratine positive cells in the indicated MPM cell lines. ( C ) Immunohistochemical analysis of BAP1, pan-cytokeratin (pan-CK), Wilms tumor-1 antigen (WT1), and calretinin (CALR) in the indicated MPM cell lines (scale bar = 100 um). ( D ) Western blot analysis showing BAP1 status of the reported MPM cell lines.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 6. SeqStain-based multiplex imaging of whole human kidney tissue provides a 20-plex image Immunofluorescent images of whole kidney tissue sections after each round of staining with unique SeqStain antibodies (as indicated) and DAPI (as indicated in the panel). Zoomed-in sections of images are presented below each panel. A serial section stained with H&E is also presented. Scale bars, 100 mum.

MA1-46028

Antibody data