Antibody data
- Antibody Data
- Antigen structure
- References [118]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [4]
- Immunohistochemistry [3]
- Other assay [62]
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Validation data
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- Product number
- MA1-744 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Actin Monoclonal Antibody (mAbGEa)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA1-744 detects actin protein in human, mouse, rat, bovine, sheep, drosophila, zebra fish (Danio rerio), yeast, arabidopsis and xenopus samples. This antibody has shown cross-reactivity with actin 1, 2, 3, 4, 7, 8, 11 and 12.
- Antibody clone number
- mAbGEa
- Concentration
- Conc. Not Determined
Submitted references circCOL1A1 Promotes the Progression of Gastric Cancer Cells through Sponging miR-145 to Enhance RABL3 Expression.
Y705 and S727 are required for the mitochondrial import and transcriptional activities of STAT3, and for regulation of stem cell proliferation.
Sulfation of glycosaminoglycans depends on the catalytic activity of lithium-inhibited phosphatase BPNT2 in vitro.
Interferon-τ regulates the expression and function of bovine leukocyte antigen by downregulating bta-miR-204.
Testicular orphan receptor 4 (TR4) promotes papillary thyroid cancer invasion via activating circ-FNLA/miR-149-5p/MMP9 signaling.
Antioxidant effects on hypoxia-induced oxidative stress and apoptosis in rat rotator cuff fibroblasts.
Divergent erythroid megakaryocyte fates in Blvrb-deficient mice establish non-overlapping cytoprotective functions during stress hematopoiesis.
miR‑205‑3p promotes lung cancer progression by targeting APBB2.
Combined effects of hyperthermia and chemotherapy on the regulate autophagy of oral squamous cell carcinoma cells under a hypoxic microenvironment.
In Silico Identification of Small Molecules as New Cdc25 Inhibitors through the Correlation between Chemosensitivity and Protein Expression Pattern.
Chronic stress physically spares but functionally impairs innate-like invariant T cells.
Adenovirus-mediated overexpression of interleukin-21 regulates the development of oral squamous cell carcinoma in vitro.
MiRNA-103/107 in Primary High-Grade Serous Ovarian Cancer and Its Clinical Significance.
Neuroretinal-Derived Caveolin-1 Promotes Endotoxin-Induced Inflammation in the Murine Retina.
Separable, Ctf4-mediated recruitment of DNA Polymerase α for initiation of DNA synthesis at replication origins and lagging-strand priming during replication elongation.
Impaired mitochondrial-endoplasmic reticulum interaction and mitophagy in Miro1-mutant neurons in Parkinson's disease.
Efficient differentiation of vascular smooth muscle cells from Wharton's Jelly mesenchymal stromal cells using human platelet lysate: A potential cell source for small blood vessel engineering.
The genetic basis of aneuploidy tolerance in wild yeast.
DNA repair by Rad52 liquid droplets.
The Inhibitory T Cell Receptors PD1 and 2B4 Are Differentially Regulated on CD4 and CD8 T Cells in a Mouse Model of Non-alcoholic Steatohepatitis.
Ethylene-mediated nitric oxide depletion pre-adapts plants to hypoxia stress.
PARP1 is a versatile factor in the regulation of mRNA stability and decay.
Epigenetic Remodeling through Downregulation of Polycomb Repressive Complex 2 Mediates Chemotherapy Resistance in Testicular Germ Cell Tumors.
HER2/HER3 regulates lactate secretion and expression of lactate receptor mRNA through the MAP3K4 associated protein GIT1.
Coupling of PARP1-mediated chromatin structural changes to transcriptional RNA polymerase II elongation and cotranscriptional splicing.
Mutations in RHOT1 Disrupt Endoplasmic Reticulum-Mitochondria Contact Sites Interfering with Calcium Homeostasis and Mitochondrial Dynamics in Parkinson's Disease.
FAM81A protein, a novel component of the postsynaptic density in adult brain.
Conserved Pbp1/Ataxin-2 regulates retrotransposon activity and connects polyglutamine expansion-driven protein aggregation to lifespan-controlling rDNA repeats.
Natural Variation in the Multidrug Efflux Pump SGE1 Underlies Ionic Liquid Tolerance in Yeast.
Combination treatment of captopril and prazosin to treat patients with gestational hypertension.
Polyphenols from Tamarix nilotica: LC⁻ESI-MS(n) Profiling and In Vivo Antifibrotic Activity.
MicroRNA expression profiling of goat peripheral blood mononuclear cells in response to peste des petits ruminants virus infection.
OsbZIP48, a HY5 Transcription Factor Ortholog, Exerts Pleiotropic Effects in Light-Regulated Development.
In silico and crystallographic studies identify key structural features of biliverdin IXβ reductase inhibitors having nanomolar potency.
An Alternate Route for Adeno-associated Virus (AAV) Entry Independent of AAV Receptor.
Cost-effective and rapid lysis of Saccharomyces cerevisiae cells for quantitative western blot analysis of proteins, including phosphorylated eIF2α.
Integration of TRPC6 and NADPH oxidase activation in lysophosphatidylcholine-induced TRPC5 externalization.
Connexin43 Forms Supramolecular Complexes through Non-Overlapping Binding Sites for Drebrin, Tubulin, and ZO-1.
Subsets of Visceral Adipose Tissue Nuclei with Distinct Levels of 5-Hydroxymethylcytosine.
A single aspartate mutation in the conserved catalytic site of Rev3L generates a hypomorphic phenotype in vivo and in vitro.
Intersection of calorie restriction and magnesium in the suppression of genome-destabilizing RNA-DNA hybrids.
The Reverse Transcriptase/RNA Maturase Protein MatR Is Required for the Splicing of Various Group II Introns in Brassicaceae Mitochondria.
The GSK3/Shaggy-Like Kinase ASKα Contributes to Pattern-Triggered Immunity.
Fimbrin phosphorylation by metaphase Cdk1 regulates actin cable dynamics in budding yeast.
A Constitutively Active Allele of Phytochrome B Maintains Circadian Robustness in the Absence of Light.
Antineoplastic effect of a novel chemopreventive agent, neokestose, on the Caco-2 cell line via inhibition of expression of nuclear factor-κB and cyclooxygenase-2.
Perinuclear tethers license telomeric DSBs for a broad kinesin- and NPC-dependent DNA repair process.
Lacrimal Gland Inflammation Deregulates Extracellular Matrix Remodeling and Alters Molecular Signature of Epithelial Stem/Progenitor Cells.
A second Las17 monomeric actin-binding motif functions in Arp2/3-dependent actin polymerization during endocytosis.
HER2/HER3 regulates extracellular acidification and cell migration through MTK1 (MEKK4).
Multiple BiP genes of Arabidopsis thaliana are required for male gametogenesis and pollen competitiveness.
Regulation of the bone-restricted IFITM-like (Bril) gene transcription by Sp and Gli family members and CpG methylation.
Metabolic regulation of CaMKII protein and caspases in Xenopus laevis egg extracts.
Protein tyrosine phosphatase-1B regulates the tyrosine phosphorylation of the adapter Grb2-associated binder 1 (Gab1) in the retina.
Cellular interactions of the cytolethal distending toxins from Escherichia coli and Haemophilus ducreyi.
Sex-dependent differences in rat brown adipose tissue mitochondrial biogenesis and insulin signaling parameters in response to an obesogenic diet.
Correlations between cytoplasmic CSE1L in neoplastic colorectal glands and depth of tumor penetration and cancer stage.
The proteolytic activity of separase in BCR-ABL-positive cells is increased by imatinib.
Unique properties of eukaryote-type actin and profilin horizontally transferred to cyanobacteria.
GATA-1 utilizes Ikaros and polycomb repressive complex 2 to suppress Hes1 and to promote erythropoiesis.
Relative contributions of afferent vagal fibers to resistance to diet-induced obesity.
Plant vegetative and animal cytoplasmic actins share functional competence for spatial development with protists.
Toll-like receptor 4 promotes tubular inflammation in diabetic nephropathy.
Chitinase-like1/pom-pom1 and its homolog CTL2 are glucan-interacting proteins important for cellulose biosynthesis in Arabidopsis.
HSF1 drives a transcriptional program distinct from heat shock to support highly malignant human cancers.
3',4'-Dihydroxyflavonol antioxidant attenuates diastolic dysfunction and cardiac remodeling in streptozotocin-induced diabetic m(Ren2)27 rats.
Conserved signaling through vascular endothelial growth (VEGF) receptor family members in murine lymphatic endothelial cells.
Human T-cell leukemia virus type 1 tax transactivates the matrix metalloproteinase 7 gene via JunD/AP-1 signaling.
High content imaging-based assay to classify estrogen receptor-α ligands based on defined mechanistic outcomes.
Analysis of the anti-tumor effect of cetuximab using protein kinetics and mouse xenograft models.
The roles of Kruppel-like factor 6 and peroxisome proliferator-activated receptor-γ in the regulation of macrophage inflammatory protein-3α at early onset of diabetes.
Small RNA-mediated regulation of iPS cell generation.
Epigenetic inactivation of calcium-sensing receptor in colorectal carcinogenesis.
SUMO-specific protease 2 in Mdm2-mediated regulation of p53.
Prevention of maternal aging-associated oocyte aneuploidy and meiotic spindle defects in mice by dietary and genetic strategies.
Effect of brimonidine on retinal and choroidal neovascularization in a mouse model of retinopathy of prematurity and laser-treated rats.
Expression and functional characterization of the RIG-I-like receptors MDA5 and LGP2 in Rainbow trout (Oncorhynchus mykiss).
Novel small-molecule inhibitor of apurinic/apyrimidinic endonuclease 1 blocks proliferation and reduces viability of glioblastoma cells.
Influence of wild-type MLL on glucocorticoid sensitivity and response to DNA-damage in pediatric acute lymphoblastic leukemia.
Differential sublocalization of actin variants within the nucleus.
Interdependence of platelet-derived growth factor and estrogen-signaling pathways in inducing neonatal rat testicular gonocytes proliferation.
Rapid upregulation of sodium-glucose transporter SGLT1 in response to intestinal sweet taste stimulation.
Interleukin-1 receptor phosphorylation activates Rho kinase to disrupt human gastric tight junctional claudin-4 during Helicobacter pylori infection.
Human cytomegalovirus infection causes premature and abnormal differentiation of human neural progenitor cells.
Retinal insulin receptor signaling in hyperosmotic stress.
Intracellularly expressed granulysin induced apoptosis in hepatoma cells and role of mitochondrial apoptotic pathway.
Insulin receptor signaling regulates actin cytoskeletal organization in developing photoreceptors.
SLIMMER (FHL1B/KyoT3) interacts with the proapoptotic protein Siva-1 (CD27BP) and delays skeletal myoblast apoptosis.
An allelic mutant series of ATM3 reveals its key role in the biogenesis of cytosolic iron-sulfur proteins in Arabidopsis.
Endosomal fusion upon SNARE knockdown is maintained by residual SNARE activity and enhanced docking.
Overexpression of kinesins mediates docetaxel resistance in breast cancer cells.
FR167653 improves renal recovery and decreases inflammation and fibrosis after renal ischemia reperfusion injury.
Influence of nephron mass and a phosphorylated 38 mitogen-activated protein kinase inhibitor on the development of early and long-term injury after renal warm ischaemia.
Bacillus cereus-induced permeability of the blood-ocular barrier during experimental endophthalmitis.
Impaired turnover of prolactin receptor contributes to transformation of human breast cells.
Insulin growth factor 1 receptor/PI3K/AKT survival pathway in outer segment membranes of rod photoreceptors.
Loss of neuroprotective survival signal in mice lacking insulin receptor gene in rod photoreceptor cells.
Characterization of Apin, a secreted protein highly expressed in tooth-associated epithelia.
The essential cytosolic iron-sulfur protein Nbp35 acts without Cfd1 partner in the green lineage.
Tight junctional disruption and apoptosis in an in vitro model of Citrobacter rodentium infection.
Stimulation of autophagy suppresses the intracellular survival of Burkholderia pseudomallei in mammalian cell lines.
Phospho-Site-Specific Antibody Microarray to Study the State of Protein Phosphorylation in the Retina.
Altered development of neuronal progenitor cells after stimulation with autistic blood sera.
Delay of photoreceptor degeneration in tubby mouse by sulforaphane.
Role of Claspin in regulation of nucleotide excision repair factor DDB2.
Cholesterol secoaldehyde, an ozonation product of cholesterol, induces amyloid aggregation and apoptosis in murine GT1-7 hypothalamic neurons.
Major HGF-mediated regenerative pathways are similarly affected in human and canine cirrhosis.
Knocking down type 2 but not type 1 calsequestrin reduces calcium sequestration and release in C2C12 skeletal muscle myotubes.
Loss of BCL-XL in rod photoreceptors: Increased susceptibility to bright light stress.
Mesenchymal-to-epithelial transition facilitates bladder cancer metastasis: role of fibroblast growth factor receptor-2.
Hydroxycamptothecin-induced apoptosis in hepatoma SMMC-7721 cells and the role of mitochondrial pathway.
Gastrin increases murine intestinal crypt regeneration following injury.
The heat shock protein 90 inhibitor, 17-allylamino-17-demethoxygeldanamycin, enhances osteoclast formation and potentiates bone metastasis of a human breast cancer cell line.
Transcriptional activation of survivin through the NF-kappaB pathway by human T-cell leukemia virus type I tax.
Interferon-alpha-induced expression of phospholipid scramblase 1 through STAT1 requires the sequential activation of protein kinase Cdelta and JNK.
Fibronectin-induced COX-2 mediates MMP-2 expression and invasiveness of rhabdomyosarcoma.
Th1-dominant shift of T cell cytokine production, and subsequent reduction of serum immunoglobulin E response by administration in vivo of plasmid expressing Txk/Rlk, a member of Tec family tyrosine kinases, in a mouse model.
Fibrogenic stresses activate different mitogen-activated protein kinase pathways in renal epithelial, endothelial or fibroblast cell populations.
Ma Y, Ren Y, Wen H, Cui C
Journal of immunology research 2021;2021:6724854
Journal of immunology research 2021;2021:6724854
Y705 and S727 are required for the mitochondrial import and transcriptional activities of STAT3, and for regulation of stem cell proliferation.
Peron M, Dinarello A, Meneghetti G, Martorano L, Betto RM, Facchinello N, Tesoriere A, Tiso N, Martello G, Argenton F
Development (Cambridge, England) 2021 Sep 1;148(17)
Development (Cambridge, England) 2021 Sep 1;148(17)
Sulfation of glycosaminoglycans depends on the catalytic activity of lithium-inhibited phosphatase BPNT2 in vitro.
Eisele BS, Luka Z, Wu AJ, Yang F, Hale AT, York JD
The Journal of biological chemistry 2021 Nov;297(5):101293
The Journal of biological chemistry 2021 Nov;297(5):101293
Interferon-τ regulates the expression and function of bovine leukocyte antigen by downregulating bta-miR-204.
Wang X, Yuan T, Yin N, Ma X, Yang Y, Yang J, Shaukat A, Deng G
Experimental and therapeutic medicine 2021 Jun;21(6):594
Experimental and therapeutic medicine 2021 Jun;21(6):594
Testicular orphan receptor 4 (TR4) promotes papillary thyroid cancer invasion via activating circ-FNLA/miR-149-5p/MMP9 signaling.
Ouyang X, Feng L, Yao L, Xiao Y, Hu X, Zhang G, Liu G, Wang Z
Molecular therapy. Nucleic acids 2021 Jun 4;24:755-767
Molecular therapy. Nucleic acids 2021 Jun 4;24:755-767
Antioxidant effects on hypoxia-induced oxidative stress and apoptosis in rat rotator cuff fibroblasts.
Kim RJ, An SH, Gwark JY, Park HB
European cells & materials 2021 Jun 11;41:680-693
European cells & materials 2021 Jun 11;41:680-693
Divergent erythroid megakaryocyte fates in Blvrb-deficient mice establish non-overlapping cytoprotective functions during stress hematopoiesis.
Nesbitt NM, Malone LE, Liu Z, Jares A, Gnatenko DV, Ma Y, Zhu W, Bahou WF
Free radical biology & medicine 2021 Feb 20;164:164-174
Free radical biology & medicine 2021 Feb 20;164:164-174
miR‑205‑3p promotes lung cancer progression by targeting APBB2.
Xu LB, Xiong J, Zhang YH, Dai Y, Ren XP, Ren YJ, Han D, Wei SH, Qi M
Molecular medicine reports 2021 Aug;24(2)
Molecular medicine reports 2021 Aug;24(2)
Combined effects of hyperthermia and chemotherapy on the regulate autophagy of oral squamous cell carcinoma cells under a hypoxic microenvironment.
Shi F, Luo D, Zhou X, Sun Q, Shen P, Wang S
Cell death discovery 2021 Aug 31;7(1):227
Cell death discovery 2021 Aug 31;7(1):227
In Silico Identification of Small Molecules as New Cdc25 Inhibitors through the Correlation between Chemosensitivity and Protein Expression Pattern.
Lauria A, Martorana A, La Monica G, Mannino S, Mannino G, Peri D, Gentile C
International journal of molecular sciences 2021 Apr 2;22(7)
International journal of molecular sciences 2021 Apr 2;22(7)
Chronic stress physically spares but functionally impairs innate-like invariant T cells.
Rudak PT, Choi J, Parkins KM, Summers KL, Jackson DN, Foster PJ, Skaro AI, Leslie K, McAlister VC, Kuchroo VK, Inoue W, Lantz O, Haeryfar SMM
Cell reports 2021 Apr 13;35(2):108979
Cell reports 2021 Apr 13;35(2):108979
Adenovirus-mediated overexpression of interleukin-21 regulates the development of oral squamous cell carcinoma in vitro.
Liu H, Liu P, Sun D, Xing D, Wang X, Yang J, Wang S
Oncology letters 2020 Sep;20(3):3006-3014
Oncology letters 2020 Sep;20(3):3006-3014
MiRNA-103/107 in Primary High-Grade Serous Ovarian Cancer and Its Clinical Significance.
Wilczynski M, Kielbik M, Senderowska D, Krawczyk T, Szymanska B, Klink M, Bieńkiewicz J, Romanowicz H, Frühauf F, Malinowski A
Cancers 2020 Sep 19;12(9)
Cancers 2020 Sep 19;12(9)
Neuroretinal-Derived Caveolin-1 Promotes Endotoxin-Induced Inflammation in the Murine Retina.
Gurley JM, Gmyrek GB, McClellan ME, Hargis EA, Hauck SM, Dozmorov MG, Wren JD, Carr DJJ, Elliott MH
Investigative ophthalmology & visual science 2020 Oct 1;61(12):19
Investigative ophthalmology & visual science 2020 Oct 1;61(12):19
Separable, Ctf4-mediated recruitment of DNA Polymerase α for initiation of DNA synthesis at replication origins and lagging-strand priming during replication elongation.
Porcella SY, Koussa NC, Tang CP, Kramer DN, Srivastava P, Smith DJ
PLoS genetics 2020 May;16(5):e1008755
PLoS genetics 2020 May;16(5):e1008755
Impaired mitochondrial-endoplasmic reticulum interaction and mitophagy in Miro1-mutant neurons in Parkinson's disease.
Berenguer-Escuder C, Grossmann D, Antony P, Arena G, Wasner K, Massart F, Jarazo J, Walter J, Schwamborn JC, Grünewald A, Krüger R
Human molecular genetics 2020 May 28;29(8):1353-1364
Human molecular genetics 2020 May 28;29(8):1353-1364
Efficient differentiation of vascular smooth muscle cells from Wharton's Jelly mesenchymal stromal cells using human platelet lysate: A potential cell source for small blood vessel engineering.
Mallis P, Papapanagiotou A, Katsimpoulas M, Kostakis A, Siasos G, Kassi E, Stavropoulos-Giokas C, Michalopoulos E
World journal of stem cells 2020 Mar 26;12(3):203-221
World journal of stem cells 2020 Mar 26;12(3):203-221
The genetic basis of aneuploidy tolerance in wild yeast.
Hose J, Escalante LE, Clowers KJ, Dutcher HA, Robinson D, Bouriakov V, Coon JJ, Shishkova E, Gasch AP
eLife 2020 Jan 7;9
eLife 2020 Jan 7;9
DNA repair by Rad52 liquid droplets.
Oshidari R, Huang R, Medghalchi M, Tse EYW, Ashgriz N, Lee HO, Wyatt H, Mekhail K
Nature communications 2020 Feb 4;11(1):695
Nature communications 2020 Feb 4;11(1):695
The Inhibitory T Cell Receptors PD1 and 2B4 Are Differentially Regulated on CD4 and CD8 T Cells in a Mouse Model of Non-alcoholic Steatohepatitis.
Hansel C, Erschfeld S, Baues M, Lammers T, Weiskirchen R, Trautwein C, Kroy DC, Drescher HK
Frontiers in pharmacology 2019;10:244
Frontiers in pharmacology 2019;10:244
Ethylene-mediated nitric oxide depletion pre-adapts plants to hypoxia stress.
Hartman S, Liu Z, van Veen H, Vicente J, Reinen E, Martopawiro S, Zhang H, van Dongen N, Bosman F, Bassel GW, Visser EJW, Bailey-Serres J, Theodoulou FL, Hebelstrup KH, Gibbs DJ, Holdsworth MJ, Sasidharan R, Voesenek LACJ
Nature communications 2019 Sep 5;10(1):4020
Nature communications 2019 Sep 5;10(1):4020
PARP1 is a versatile factor in the regulation of mRNA stability and decay.
Matveeva EA, Mathbout LF, Fondufe-Mittendorf YN
Scientific reports 2019 Mar 6;9(1):3722
Scientific reports 2019 Mar 6;9(1):3722
Epigenetic Remodeling through Downregulation of Polycomb Repressive Complex 2 Mediates Chemotherapy Resistance in Testicular Germ Cell Tumors.
Singh R, Fazal Z, Corbet AK, Bikorimana E, Rodriguez JC, Khan EM, Shahid K, Freemantle SJ, Spinella MJ
Cancers 2019 Jun 8;11(6)
Cancers 2019 Jun 8;11(6)
HER2/HER3 regulates lactate secretion and expression of lactate receptor mRNA through the MAP3K4 associated protein GIT1.
Garcia-Flores AE, Sollome JJ, Thavathiru E, Bower JL, Vaillancourt RR
Scientific reports 2019 Jul 25;9(1):10823
Scientific reports 2019 Jul 25;9(1):10823
Coupling of PARP1-mediated chromatin structural changes to transcriptional RNA polymerase II elongation and cotranscriptional splicing.
Matveeva EA, Al-Tinawi QMH, Rouchka EC, Fondufe-Mittendorf YN
Epigenetics & chromatin 2019 Feb 18;12(1):15
Epigenetics & chromatin 2019 Feb 18;12(1):15
Mutations in RHOT1 Disrupt Endoplasmic Reticulum-Mitochondria Contact Sites Interfering with Calcium Homeostasis and Mitochondrial Dynamics in Parkinson's Disease.
Grossmann D, Berenguer-Escuder C, Bellet ME, Scheibner D, Bohler J, Massart F, Rapaport D, Skupin A, Fouquier d'Hérouël A, Sharma M, Ghelfi J, Raković A, Lichtner P, Antony P, Glaab E, May P, Dimmer KS, Fitzgerald JC, Grünewald A, Krüger R
Antioxidants & redox signaling 2019 Dec 1;31(16):1213-1234
Antioxidants & redox signaling 2019 Dec 1;31(16):1213-1234
FAM81A protein, a novel component of the postsynaptic density in adult brain.
Dosemeci A, Loo HK, Toy D, Winters CA, Reese TS, Tao-Cheng JH
Neuroscience letters 2019 Apr 23;699:122-126
Neuroscience letters 2019 Apr 23;699:122-126
Conserved Pbp1/Ataxin-2 regulates retrotransposon activity and connects polyglutamine expansion-driven protein aggregation to lifespan-controlling rDNA repeats.
Ostrowski LA, Hall AC, Szafranski KJ, Oshidari R, Abraham KJ, Chan JNY, Krustev C, Zhang K, Wang A, Liu Y, Guo R, Mekhail K
Communications biology 2018;1:187
Communications biology 2018;1:187
Natural Variation in the Multidrug Efflux Pump SGE1 Underlies Ionic Liquid Tolerance in Yeast.
Higgins DA, Young MKM, Tremaine M, Sardi M, Fletcher JM, Agnew M, Liu L, Dickinson Q, Peris D, Wrobel RL, Hittinger CT, Gasch AP, Singer SW, Simmons BA, Landick R, Thelen MP, Sato TK
Genetics 2018 Sep;210(1):219-234
Genetics 2018 Sep;210(1):219-234
Combination treatment of captopril and prazosin to treat patients with gestational hypertension.
Hong B, Ding X, Iia H, Zhang J
Experimental and therapeutic medicine 2018 Oct;16(4):3694-3702
Experimental and therapeutic medicine 2018 Oct;16(4):3694-3702
Polyphenols from Tamarix nilotica: LC⁻ESI-MS(n) Profiling and In Vivo Antifibrotic Activity.
Sekkien A, Swilam N, Ebada SS, Esmat A, El-Khatib AH, Linscheid MW, Singab AN
Molecules (Basel, Switzerland) 2018 Jun 11;23(6)
Molecules (Basel, Switzerland) 2018 Jun 11;23(6)
MicroRNA expression profiling of goat peripheral blood mononuclear cells in response to peste des petits ruminants virus infection.
Qi X, Wang T, Xue Q, Li Z, Yang B, Wang J
Veterinary research 2018 Jul 16;49(1):62
Veterinary research 2018 Jul 16;49(1):62
OsbZIP48, a HY5 Transcription Factor Ortholog, Exerts Pleiotropic Effects in Light-Regulated Development.
Burman N, Bhatnagar A, Khurana JP
Plant physiology 2018 Feb;176(2):1262-1285
Plant physiology 2018 Feb;176(2):1262-1285
In silico and crystallographic studies identify key structural features of biliverdin IXβ reductase inhibitors having nanomolar potency.
Nesbitt NM, Zheng X, Li Z, Manso JA, Yen WY, Malone LE, Ripoll-Rozada J, Pereira PJB, Mantle TJ, Wang J, Bahou WF
The Journal of biological chemistry 2018 Apr 13;293(15):5431-5446
The Journal of biological chemistry 2018 Apr 13;293(15):5431-5446
An Alternate Route for Adeno-associated Virus (AAV) Entry Independent of AAV Receptor.
Dudek AM, Pillay S, Puschnik AS, Nagamine CM, Cheng F, Qiu J, Carette JE, Vandenberghe LH
Journal of virology 2018 Apr 1;92(7)
Journal of virology 2018 Apr 1;92(7)
Cost-effective and rapid lysis of Saccharomyces cerevisiae cells for quantitative western blot analysis of proteins, including phosphorylated eIF2α.
Lee SJ, Ramesh R, de Boor V, Gebler JM, Silva RC, Sattlegger E
Yeast (Chichester, England) 2017 Sep;34(9):371-382
Yeast (Chichester, England) 2017 Sep;34(9):371-382
Integration of TRPC6 and NADPH oxidase activation in lysophosphatidylcholine-induced TRPC5 externalization.
Chaudhuri P, Rosenbaum MA, Birnbaumer L, Graham LM
American journal of physiology. Cell physiology 2017 Nov 1;313(5):C541-C555
American journal of physiology. Cell physiology 2017 Nov 1;313(5):C541-C555
Connexin43 Forms Supramolecular Complexes through Non-Overlapping Binding Sites for Drebrin, Tubulin, and ZO-1.
Ambrosi C, Ren C, Spagnol G, Cavin G, Cone A, Grintsevich EE, Sosinsky GE, Sorgen PL
PloS one 2016;11(6):e0157073
PloS one 2016;11(6):e0157073
Subsets of Visceral Adipose Tissue Nuclei with Distinct Levels of 5-Hydroxymethylcytosine.
Yu P, Ji L, Lee KJ, Yu M, He C, Ambati S, McKinney EC, Jackson C, Baile CA, Schmitz RJ, Meagher RB
PloS one 2016;11(5):e0154949
PloS one 2016;11(5):e0154949
A single aspartate mutation in the conserved catalytic site of Rev3L generates a hypomorphic phenotype in vivo and in vitro.
Fritzen R, Delbos F, De Smet A, Palancade B, Canman CE, Aoufouchi S, Weill JC, Reynaud CA, Storck S
DNA repair 2016 Oct;46:37-46
DNA repair 2016 Oct;46:37-46
Intersection of calorie restriction and magnesium in the suppression of genome-destabilizing RNA-DNA hybrids.
Abraham KJ, Chan JN, Salvi JS, Ho B, Hall A, Vidya E, Guo R, Killackey SA, Liu N, Lee JE, Brown GW, Mekhail K
Nucleic acids research 2016 Oct 14;44(18):8870-8884
Nucleic acids research 2016 Oct 14;44(18):8870-8884
The Reverse Transcriptase/RNA Maturase Protein MatR Is Required for the Splicing of Various Group II Introns in Brassicaceae Mitochondria.
Sultan LD, Mileshina D, Grewe F, Rolle K, Abudraham S, Głodowicz P, Niazi AK, Keren I, Shevtsov S, Klipcan L, Barciszewski J, Mower JP, Dietrich A, Ostersetzer-Biran O
The Plant cell 2016 Nov;28(11):2805-2829
The Plant cell 2016 Nov;28(11):2805-2829
The GSK3/Shaggy-Like Kinase ASKα Contributes to Pattern-Triggered Immunity.
Stampfl H, Fritz M, Dal Santo S, Jonak C
Plant physiology 2016 Jun;171(2):1366-77
Plant physiology 2016 Jun;171(2):1366-77
Fimbrin phosphorylation by metaphase Cdk1 regulates actin cable dynamics in budding yeast.
Miao Y, Han X, Zheng L, Xie Y, Mu Y, Yates JR 3rd, Drubin DG
Nature communications 2016 Apr 12;7:11265
Nature communications 2016 Apr 12;7:11265
A Constitutively Active Allele of Phytochrome B Maintains Circadian Robustness in the Absence of Light.
Jones MA, Hu W, Litthauer S, Lagarias JC, Harmer SL
Plant physiology 2015 Sep;169(1):814-25
Plant physiology 2015 Sep;169(1):814-25
Antineoplastic effect of a novel chemopreventive agent, neokestose, on the Caco-2 cell line via inhibition of expression of nuclear factor-κB and cyclooxygenase-2.
Lee SM, Chang JY, Wu JS, Sheu DC
Molecular medicine reports 2015 Jul;12(1):1114-8
Molecular medicine reports 2015 Jul;12(1):1114-8
Perinuclear tethers license telomeric DSBs for a broad kinesin- and NPC-dependent DNA repair process.
Chung DK, Chan JN, Strecker J, Zhang W, Ebrahimi-Ardebili S, Lu T, Abraham KJ, Durocher D, Mekhail K
Nature communications 2015 Jul 24;6:7742
Nature communications 2015 Jul 24;6:7742
Lacrimal Gland Inflammation Deregulates Extracellular Matrix Remodeling and Alters Molecular Signature of Epithelial Stem/Progenitor Cells.
Umazume T, Thomas WM, Campbell S, Aluri H, Thotakura S, Zoukhri D, Makarenkova HP
Investigative ophthalmology & visual science 2015 Dec;56(13):8392-402
Investigative ophthalmology & visual science 2015 Dec;56(13):8392-402
A second Las17 monomeric actin-binding motif functions in Arp2/3-dependent actin polymerization during endocytosis.
Feliciano D, Tolsma TO, Farrell KB, Aradi A, Di Pietro SM
Traffic (Copenhagen, Denmark) 2015 Apr;16(4):379-97
Traffic (Copenhagen, Denmark) 2015 Apr;16(4):379-97
HER2/HER3 regulates extracellular acidification and cell migration through MTK1 (MEKK4).
Sollome JJ, Thavathiru E, Camenisch TD, Vaillancourt RR
Cellular signalling 2014 Jan;26(1):70-82
Cellular signalling 2014 Jan;26(1):70-82
Multiple BiP genes of Arabidopsis thaliana are required for male gametogenesis and pollen competitiveness.
Maruyama D, Sugiyama T, Endo T, Nishikawa S
Plant & cell physiology 2014 Apr;55(4):801-10
Plant & cell physiology 2014 Apr;55(4):801-10
Regulation of the bone-restricted IFITM-like (Bril) gene transcription by Sp and Gli family members and CpG methylation.
Kasaai B, Gaumond MH, Moffatt P
The Journal of biological chemistry 2013 May 10;288(19):13278-94
The Journal of biological chemistry 2013 May 10;288(19):13278-94
Metabolic regulation of CaMKII protein and caspases in Xenopus laevis egg extracts.
McCoy F, Darbandi R, Chen SI, Eckard L, Dodd K, Jones K, Baucum AJ 2nd, Gibbons JA, Lin SH, Colbran RJ, Nutt LK
The Journal of biological chemistry 2013 Mar 29;288(13):8838-48
The Journal of biological chemistry 2013 Mar 29;288(13):8838-48
Protein tyrosine phosphatase-1B regulates the tyrosine phosphorylation of the adapter Grb2-associated binder 1 (Gab1) in the retina.
Rajala A, Dilly AK, Rajala RV
Cell communication and signaling : CCS 2013 Mar 22;11(1):20
Cell communication and signaling : CCS 2013 Mar 22;11(1):20
Cellular interactions of the cytolethal distending toxins from Escherichia coli and Haemophilus ducreyi.
Gargi A, Tamilselvam B, Powers B, Prouty MG, Lincecum T, Eshraghi A, Maldonado-Arocho FJ, Wilson BA, Bradley KA, Blanke SR
The Journal of biological chemistry 2013 Mar 15;288(11):7492-7505
The Journal of biological chemistry 2013 Mar 15;288(11):7492-7505
Sex-dependent differences in rat brown adipose tissue mitochondrial biogenesis and insulin signaling parameters in response to an obesogenic diet.
Nadal-Casellas A, Bauzá-Thorbrügge M, Proenza AM, Gianotti M, Lladó I
Molecular and cellular biochemistry 2013 Jan;373(1-2):125-35
Molecular and cellular biochemistry 2013 Jan;373(1-2):125-35
Correlations between cytoplasmic CSE1L in neoplastic colorectal glands and depth of tumor penetration and cancer stage.
Tai CJ, Su TC, Jiang MC, Chen HC, Shen SC, Lee WR, Liao CF, Chen YC, Lin SH, Li LT, Shen KH, Yeh CM, Yeh KT, Lee CH, Shih HY, Chang CC
Journal of translational medicine 2013 Jan 31;11:29
Journal of translational medicine 2013 Jan 31;11:29
The proteolytic activity of separase in BCR-ABL-positive cells is increased by imatinib.
Haaß W, Stehle M, Nittka S, Giehl M, Schrotz-King P, Fabarius A, Hofmann WK, Seifarth W
PloS one 2012;7(8):e42863
PloS one 2012;7(8):e42863
Unique properties of eukaryote-type actin and profilin horizontally transferred to cyanobacteria.
Guljamow A, Delissen F, Baumann O, Thünemann AF, Dittmann E
PloS one 2012;7(1):e29926
PloS one 2012;7(1):e29926
GATA-1 utilizes Ikaros and polycomb repressive complex 2 to suppress Hes1 and to promote erythropoiesis.
Ross J, Mavoungou L, Bresnick EH, Milot E
Molecular and cellular biology 2012 Sep;32(18):3624-38
Molecular and cellular biology 2012 Sep;32(18):3624-38
Relative contributions of afferent vagal fibers to resistance to diet-induced obesity.
Stearns AT, Balakrishnan A, Radmanesh A, Ashley SW, Rhoads DB, Tavakkolizadeh A
Digestive diseases and sciences 2012 May;57(5):1281-90
Digestive diseases and sciences 2012 May;57(5):1281-90
Plant vegetative and animal cytoplasmic actins share functional competence for spatial development with protists.
Kandasamy MK, McKinney EC, Roy E, Meagher RB
The Plant cell 2012 May;24(5):2041-57
The Plant cell 2012 May;24(5):2041-57
Toll-like receptor 4 promotes tubular inflammation in diabetic nephropathy.
Lin M, Yiu WH, Wu HJ, Chan LY, Leung JC, Au WS, Chan KW, Lai KN, Tang SC
Journal of the American Society of Nephrology : JASN 2012 Jan;23(1):86-102
Journal of the American Society of Nephrology : JASN 2012 Jan;23(1):86-102
Chitinase-like1/pom-pom1 and its homolog CTL2 are glucan-interacting proteins important for cellulose biosynthesis in Arabidopsis.
Sánchez-Rodríguez C, Bauer S, Hématy K, Saxe F, Ibáñez AB, Vodermaier V, Konlechner C, Sampathkumar A, Rüggeberg M, Aichinger E, Neumetzler L, Burgert I, Somerville C, Hauser MT, Persson S
The Plant cell 2012 Feb;24(2):589-607
The Plant cell 2012 Feb;24(2):589-607
HSF1 drives a transcriptional program distinct from heat shock to support highly malignant human cancers.
Mendillo ML, Santagata S, Koeva M, Bell GW, Hu R, Tamimi RM, Fraenkel E, Ince TA, Whitesell L, Lindquist S
Cell 2012 Aug 3;150(3):549-62
Cell 2012 Aug 3;150(3):549-62
3',4'-Dihydroxyflavonol antioxidant attenuates diastolic dysfunction and cardiac remodeling in streptozotocin-induced diabetic m(Ren2)27 rats.
Khong FL, Zhang Y, Edgley AJ, Qi W, Connelly KA, Woodman OL, Krum H, Kelly DJ
PloS one 2011;6(7):e22777
PloS one 2011;6(7):e22777
Conserved signaling through vascular endothelial growth (VEGF) receptor family members in murine lymphatic endothelial cells.
Coso S, Zeng Y, Sooraj D, Williams ED
Experimental cell research 2011 Oct 15;317(17):2397-407
Experimental cell research 2011 Oct 15;317(17):2397-407
Human T-cell leukemia virus type 1 tax transactivates the matrix metalloproteinase 7 gene via JunD/AP-1 signaling.
Nakachi S, Nakazato T, Ishikawa C, Kimura R, Mann DA, Senba M, Masuzaki H, Mori N
Biochimica et biophysica acta 2011 May;1813(5):731-41
Biochimica et biophysica acta 2011 May;1813(5):731-41
High content imaging-based assay to classify estrogen receptor-α ligands based on defined mechanistic outcomes.
Ashcroft FJ, Newberg JY, Jones ED, Mikic I, Mancini MA
Gene 2011 May 15;477(1-2):42-52
Gene 2011 May 15;477(1-2):42-52
Analysis of the anti-tumor effect of cetuximab using protein kinetics and mouse xenograft models.
Matsuo T, Nishizuka SS, Ishida K, Iwaya T, Ikeda M, Wakabayashi G
BMC research notes 2011 May 10;4:140
BMC research notes 2011 May 10;4:140
The roles of Kruppel-like factor 6 and peroxisome proliferator-activated receptor-γ in the regulation of macrophage inflammatory protein-3α at early onset of diabetes.
Qi W, Holian J, Tan CY, Kelly DJ, Chen XM, Pollock CA
The international journal of biochemistry & cell biology 2011 Mar;43(3):383-92
The international journal of biochemistry & cell biology 2011 Mar;43(3):383-92
Small RNA-mediated regulation of iPS cell generation.
Li Z, Yang CS, Nakashima K, Rana TM
The EMBO journal 2011 Mar 2;30(5):823-34
The EMBO journal 2011 Mar 2;30(5):823-34
Epigenetic inactivation of calcium-sensing receptor in colorectal carcinogenesis.
Hizaki K, Yamamoto H, Taniguchi H, Adachi Y, Nakazawa M, Tanuma T, Kato N, Sukawa Y, Sanchez JV, Suzuki H, Sasaki S, Imai K, Shinomura Y
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 2011 Jun;24(6):876-84
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 2011 Jun;24(6):876-84
SUMO-specific protease 2 in Mdm2-mediated regulation of p53.
Jiang M, Chiu SY, Hsu W
Cell death and differentiation 2011 Jun;18(6):1005-15
Cell death and differentiation 2011 Jun;18(6):1005-15
Prevention of maternal aging-associated oocyte aneuploidy and meiotic spindle defects in mice by dietary and genetic strategies.
Selesniemi K, Lee HJ, Muhlhauser A, Tilly JL
Proceedings of the National Academy of Sciences of the United States of America 2011 Jul 26;108(30):12319-24
Proceedings of the National Academy of Sciences of the United States of America 2011 Jul 26;108(30):12319-24
Effect of brimonidine on retinal and choroidal neovascularization in a mouse model of retinopathy of prematurity and laser-treated rats.
Kusari J, Padillo E, Zhou SX, Bai Y, Wang J, Song Z, Zhu M, Le YZ, Gil DW
Investigative ophthalmology & visual science 2011 Jul 20;52(8):5424-31
Investigative ophthalmology & visual science 2011 Jul 20;52(8):5424-31
Expression and functional characterization of the RIG-I-like receptors MDA5 and LGP2 in Rainbow trout (Oncorhynchus mykiss).
Chang M, Collet B, Nie P, Lester K, Campbell S, Secombes CJ, Zou J
Journal of virology 2011 Aug;85(16):8403-12
Journal of virology 2011 Aug;85(16):8403-12
Novel small-molecule inhibitor of apurinic/apyrimidinic endonuclease 1 blocks proliferation and reduces viability of glioblastoma cells.
Bapat A, Glass LS, Luo M, Fishel ML, Long EC, Georgiadis MM, Kelley MR
The Journal of pharmacology and experimental therapeutics 2010 Sep 1;334(3):988-98
The Journal of pharmacology and experimental therapeutics 2010 Sep 1;334(3):988-98
Influence of wild-type MLL on glucocorticoid sensitivity and response to DNA-damage in pediatric acute lymphoblastic leukemia.
Beesley AH, Rampellini JL, Palmer ML, Heng JY, Samuels AL, Firth MJ, Ford J, Kees UR
Molecular cancer 2010 Oct 28;9:284
Molecular cancer 2010 Oct 28;9:284
Differential sublocalization of actin variants within the nucleus.
Kandasamy MK, McKinney EC, Meagher RB
Cytoskeleton (Hoboken, N.J.) 2010 Nov;67(11):729-43
Cytoskeleton (Hoboken, N.J.) 2010 Nov;67(11):729-43
Interdependence of platelet-derived growth factor and estrogen-signaling pathways in inducing neonatal rat testicular gonocytes proliferation.
Thuillier R, Mazer M, Manku G, Boisvert A, Wang Y, Culty M
Biology of reproduction 2010 May;82(5):825-36
Biology of reproduction 2010 May;82(5):825-36
Rapid upregulation of sodium-glucose transporter SGLT1 in response to intestinal sweet taste stimulation.
Stearns AT, Balakrishnan A, Rhoads DB, Tavakkolizadeh A
Annals of surgery 2010 May;251(5):865-71
Annals of surgery 2010 May;251(5):865-71
Interleukin-1 receptor phosphorylation activates Rho kinase to disrupt human gastric tight junctional claudin-4 during Helicobacter pylori infection.
Lapointe TK, O'Connor PM, Jones NL, Menard D, Buret AG
Cellular microbiology 2010 May 1;12(5):692-703
Cellular microbiology 2010 May 1;12(5):692-703
Human cytomegalovirus infection causes premature and abnormal differentiation of human neural progenitor cells.
Luo MH, Hannemann H, Kulkarni AS, Schwartz PH, O'Dowd JM, Fortunato EA
Journal of virology 2010 Apr;84(7):3528-41
Journal of virology 2010 Apr;84(7):3528-41
Retinal insulin receptor signaling in hyperosmotic stress.
Rajala RV, Ivanovic I, Dilly AK
Vitamins and hormones 2009;80:583-612
Vitamins and hormones 2009;80:583-612
Intracellularly expressed granulysin induced apoptosis in hepatoma cells and role of mitochondrial apoptotic pathway.
Yi Z, Fu Y, Jin G, Li M, Zhang X, Song W
Cellular immunology 2009;255(1-2):76-81
Cellular immunology 2009;255(1-2):76-81
Insulin receptor signaling regulates actin cytoskeletal organization in developing photoreceptors.
Rajala RV, Rajala A, Brush RS, Rotstein NP, Politi LE
Journal of neurochemistry 2009 Sep;110(5):1648-60
Journal of neurochemistry 2009 Sep;110(5):1648-60
SLIMMER (FHL1B/KyoT3) interacts with the proapoptotic protein Siva-1 (CD27BP) and delays skeletal myoblast apoptosis.
Cottle DL, McGrath MJ, Wilding BR, Cowling BS, Kane JM, D'Arcy CE, Holdsworth M, Hatzinisiriou I, Prescott M, Brown S, Mitchell CA
The Journal of biological chemistry 2009 Sep 25;284(39):26964-77
The Journal of biological chemistry 2009 Sep 25;284(39):26964-77
An allelic mutant series of ATM3 reveals its key role in the biogenesis of cytosolic iron-sulfur proteins in Arabidopsis.
Bernard DG, Cheng Y, Zhao Y, Balk J
Plant physiology 2009 Oct;151(2):590-602
Plant physiology 2009 Oct;151(2):590-602
Endosomal fusion upon SNARE knockdown is maintained by residual SNARE activity and enhanced docking.
Bethani I, Werner A, Kadian C, Geumann U, Jahn R, Rizzoli SO
Traffic (Copenhagen, Denmark) 2009 Oct;10(10):1543-59
Traffic (Copenhagen, Denmark) 2009 Oct;10(10):1543-59
Overexpression of kinesins mediates docetaxel resistance in breast cancer cells.
De S, Cipriano R, Jackson MW, Stark GR
Cancer research 2009 Oct 15;69(20):8035-42
Cancer research 2009 Oct 15;69(20):8035-42
FR167653 improves renal recovery and decreases inflammation and fibrosis after renal ischemia reperfusion injury.
Cau J, Favreau F, Zhang K, Febrer G, de la Motte GR, Ricco JB, Goujon JM, Hauet T
Journal of vascular surgery 2009 Mar;49(3):728-40
Journal of vascular surgery 2009 Mar;49(3):728-40
Influence of nephron mass and a phosphorylated 38 mitogen-activated protein kinase inhibitor on the development of early and long-term injury after renal warm ischaemia.
Jayle C, Faure JP, Thuillier R, Goujon JM, Richer JP, Hauet T
The British journal of surgery 2009 Jul;96(7):799-808
The British journal of surgery 2009 Jul;96(7):799-808
Bacillus cereus-induced permeability of the blood-ocular barrier during experimental endophthalmitis.
Moyer AL, Ramadan RT, Novosad BD, Astley R, Callegan MC
Investigative ophthalmology & visual science 2009 Aug;50(8):3783-93
Investigative ophthalmology & visual science 2009 Aug;50(8):3783-93
Impaired turnover of prolactin receptor contributes to transformation of human breast cells.
Plotnikov A, Varghese B, Tran TH, Liu C, Rui H, Fuchs SY
Cancer research 2009 Apr 1;69(7):3165-72
Cancer research 2009 Apr 1;69(7):3165-72
Insulin growth factor 1 receptor/PI3K/AKT survival pathway in outer segment membranes of rod photoreceptors.
Dilly AK, Rajala RV
Investigative ophthalmology & visual science 2008 Nov;49(11):4765-73
Investigative ophthalmology & visual science 2008 Nov;49(11):4765-73
Loss of neuroprotective survival signal in mice lacking insulin receptor gene in rod photoreceptor cells.
Rajala A, Tanito M, Le YZ, Kahn CR, Rajala RV
The Journal of biological chemistry 2008 Jul 11;283(28):19781-92
The Journal of biological chemistry 2008 Jul 11;283(28):19781-92
Characterization of Apin, a secreted protein highly expressed in tooth-associated epithelia.
Moffatt P, Smith CE, St-Arnaud R, Nanci A
Journal of cellular biochemistry 2008 Feb 15;103(3):941-56
Journal of cellular biochemistry 2008 Feb 15;103(3):941-56
The essential cytosolic iron-sulfur protein Nbp35 acts without Cfd1 partner in the green lineage.
Bych K, Netz DJ, Vigani G, Bill E, Lill R, Pierik AJ, Balk J
The Journal of biological chemistry 2008 Dec 19;283(51):35797-804
The Journal of biological chemistry 2008 Dec 19;283(51):35797-804
Tight junctional disruption and apoptosis in an in vitro model of Citrobacter rodentium infection.
Flynn AN, Buret AG
Microbial pathogenesis 2008 Aug;45(2):98-104
Microbial pathogenesis 2008 Aug;45(2):98-104
Stimulation of autophagy suppresses the intracellular survival of Burkholderia pseudomallei in mammalian cell lines.
Cullinane M, Gong L, Li X, Lazar-Adler N, Tra T, Wolvetang E, Prescott M, Boyce JD, Devenish RJ, Adler B
Autophagy 2008 Aug;4(6):744-53
Autophagy 2008 Aug;4(6):744-53
Phospho-Site-Specific Antibody Microarray to Study the State of Protein Phosphorylation in the Retina.
Rajala RV
Journal of proteomics & bioinformatics 2008 Aug 13;1:242
Journal of proteomics & bioinformatics 2008 Aug 13;1:242
Altered development of neuronal progenitor cells after stimulation with autistic blood sera.
Mazur-Kolecka B, Cohen IL, Jenkins EC, Kaczmarski W, Flory M, Frackowiak J
Brain research 2007 Sep 7;1168:11-20
Brain research 2007 Sep 7;1168:11-20
Delay of photoreceptor degeneration in tubby mouse by sulforaphane.
Kong L, Tanito M, Huang Z, Li F, Zhou X, Zaharia A, Yodoi J, McGinnis JF, Cao W
Journal of neurochemistry 2007 May;101(4):1041-52
Journal of neurochemistry 2007 May;101(4):1041-52
Role of Claspin in regulation of nucleotide excision repair factor DDB2.
Praetorius-Ibba M, Wang QE, Wani G, El-Mahdy MA, Zhu Q, Qin S, Wani AA
DNA repair 2007 May 1;6(5):578-87
DNA repair 2007 May 1;6(5):578-87
Cholesterol secoaldehyde, an ozonation product of cholesterol, induces amyloid aggregation and apoptosis in murine GT1-7 hypothalamic neurons.
Sathishkumar K, Xi X, Martin R, Uppu RM
Journal of Alzheimer's disease : JAD 2007 Jun;11(3):261-74
Journal of Alzheimer's disease : JAD 2007 Jun;11(3):261-74
Major HGF-mediated regenerative pathways are similarly affected in human and canine cirrhosis.
Spee B, Arends B, van den Ingh TS, Roskams T, Rothuizen J, Penning LC
Comparative hepatology 2007 Jul 31;6:8
Comparative hepatology 2007 Jul 31;6:8
Knocking down type 2 but not type 1 calsequestrin reduces calcium sequestration and release in C2C12 skeletal muscle myotubes.
Wang Y, Xu L, Duan H, Pasek DA, Eu JP, Meissner G
The Journal of biological chemistry 2006 Jun 2;281(22):15572-81
The Journal of biological chemistry 2006 Jun 2;281(22):15572-81
Loss of BCL-XL in rod photoreceptors: Increased susceptibility to bright light stress.
Zheng L, Anderson RE, Agbaga MP, Rucker EB 3rd, Le YZ
Investigative ophthalmology & visual science 2006 Dec;47(12):5583-9
Investigative ophthalmology & visual science 2006 Dec;47(12):5583-9
Mesenchymal-to-epithelial transition facilitates bladder cancer metastasis: role of fibroblast growth factor receptor-2.
Chaffer CL, Brennan JP, Slavin JL, Blick T, Thompson EW, Williams ED
Cancer research 2006 Dec 1;66(23):11271-8
Cancer research 2006 Dec 1;66(23):11271-8
Hydroxycamptothecin-induced apoptosis in hepatoma SMMC-7721 cells and the role of mitochondrial pathway.
Fu YR, Yi ZJ, Yan YR, Qiu ZY
Mitochondrion 2006 Aug;6(4):211-7
Mitochondrion 2006 Aug;6(4):211-7
Gastrin increases murine intestinal crypt regeneration following injury.
Ottewell PD, Duckworth CA, Varro A, Dimaline R, Wang TC, Watson AJ, Dockray GJ, Pritchard DM
Gastroenterology 2006 Apr;130(4):1169-80
Gastroenterology 2006 Apr;130(4):1169-80
The heat shock protein 90 inhibitor, 17-allylamino-17-demethoxygeldanamycin, enhances osteoclast formation and potentiates bone metastasis of a human breast cancer cell line.
Price JT, Quinn JM, Sims NA, Vieusseux J, Waldeck K, Docherty SE, Myers D, Nakamura A, Waltham MC, Gillespie MT, Thompson EW
Cancer research 2005 Jun 1;65(11):4929-38
Cancer research 2005 Jun 1;65(11):4929-38
Transcriptional activation of survivin through the NF-kappaB pathway by human T-cell leukemia virus type I tax.
Kawakami H, Tomita M, Matsuda T, Ohta T, Tanaka Y, Fujii M, Hatano M, Tokuhisa T, Mori N
International journal of cancer 2005 Jul 20;115(6):967-74
International journal of cancer 2005 Jul 20;115(6):967-74
Interferon-alpha-induced expression of phospholipid scramblase 1 through STAT1 requires the sequential activation of protein kinase Cdelta and JNK.
Zhao KW, Li D, Zhao Q, Huang Y, Silverman RH, Sims PJ, Chen GQ
The Journal of biological chemistry 2005 Dec 30;280(52):42707-14
The Journal of biological chemistry 2005 Dec 30;280(52):42707-14
Fibronectin-induced COX-2 mediates MMP-2 expression and invasiveness of rhabdomyosarcoma.
Ito H, Duxbury M, Benoit E, Farivar RS, Gardner-Thorpe J, Zinner MJ, Ashley SW, Whang EE
Biochemical and biophysical research communications 2004 May 28;318(2):594-600
Biochemical and biophysical research communications 2004 May 28;318(2):594-600
Th1-dominant shift of T cell cytokine production, and subsequent reduction of serum immunoglobulin E response by administration in vivo of plasmid expressing Txk/Rlk, a member of Tec family tyrosine kinases, in a mouse model.
Takeno M, Yoshikawa H, Kurokawa M, Takeba Y, Kashiwakura JI, Sakaguchi M, Yasueda H, Suzuki N
Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology 2004 Jun;34(6):965-70
Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology 2004 Jun;34(6):965-70
Fibrogenic stresses activate different mitogen-activated protein kinase pathways in renal epithelial, endothelial or fibroblast cell populations.
Pat BK, Cuttle L, Watters D, Yang T, Johnson DW, Gobe GC
Nephrology (Carlton, Vic.) 2003 Aug;8(4):196-204
Nephrology (Carlton, Vic.) 2003 Aug;8(4):196-204
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western Blot was performed using Anti-Actin Monoclonal Antibody (mAbGEa) (Product # MA1-744) and a ~40 kDa band corresponding to Actin, cytoplasmic 1 was observed along with an uncharacterised band (*) at ~30 kDa across cell lines and tissues tested. Whole cell extracts (30 µg lysate) of HeLa (Lane 1), A549 (Lane 2), A-431 (Lane 3), IMR-90 (Lane 4), PANC1 (Lane 5), DU 145 (Lane 6), LNCaP (Lane 7), PC-12 (Lane 8), NIH/3T3 (Lane 9), Mouse Kidney (Lane 10), Rat Kidney (Lane 11) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # LC2001) by iBlot® 2 Dry Blotting System (Product # IB21001). The Blot was probed with the primary antibody (1:10000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
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- Immunofluorescent analysis of Actin using Anti-Actin Monoclonal Antibody (mAbGEa) (Product # MA1-744) shows staining in A375 Cells. Actin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Actin (Product # MA1-744) at a dilution of 1:20 over night at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503, Goat Anti-Mouse). Images were taken at 60X magnification.
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- Immunofluorescent analysis of Actin using Anti-Actin Monoclonal Antibody (mAbGEa) (Product # MA1-744) shows staining in Hela Cells. Actin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Actin (Product # MA1-744) at a dilution of 1:20 over night at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503, Goat Anti-Mouse). Images were taken at 60X magnification.
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- Immunofluorescent analysis of Actin (green) in 3T3 cells. The cells were permeabilized with 0.1% Triton X-100 in TBS for 15 minutes, and blocked with 3% Blocker BSA in PBS (Product # 37525) for 15 minutes at room temperature. Cells were stained with an Actin mouse monoclonal antibody (Product # MA1-744), at a dilution of 1:100 in blocking buffer for at least 1 hour at room temperature, and then incubated with a Goat anti-Mouse IgG Superclonal secondary antibody, Alexa Fluor 488 conjugate (Product # A28175) at a dilution of 1:1000 for 30 minutes at room temperature (green). Nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of Actin, cytoplasmic 1 was performed using 70% confluent log phase DU 145 cells. The cells were fixed with 4% paraformaldehyde for 5 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Actin Monoclonal Antibody (mAbGEa) (Product # MA1-744) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 647 (Product # A32787), (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing Nucleus and cytoplasm localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60x magnification.
Supportive validation
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- Immunohistochemistry was performed on cancer biopsies of deparaffinized Human colon carcinoma tissues. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:1000 with a mouse monoclonal antibody recognizing Anti-Actin (Product # MA1-744) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
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- Immunohistochemistry was performed on normal deparaffinized Human skeletal muscle tissues. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:1000 with a mouse monoclonal antibody recognizing Anti-Actin (Product # MA1-744) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry was performed on normal deparaffinized Human tonsil tissue tissues. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:1000 with a mouse monoclonal antibody recognizing Anti-Actin (Product # MA1-744) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
Supportive validation
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- Invitrogen Antibodies (provider)
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- Invitrogen Antibodies (provider)
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- Figure 1 Characterization of PPRV infection in goat PBMC. A Morphological changes in goat PBMC at different time points after PPRV infection (MOI = 1), with mock-infected cells as a control. B Western blot analysis of N protein in PPRV-infected and mock-infected goat PBMC. Equal amounts of protein from PPRV to mock-infected cells were separated using SDS-PAGE and transferred to PVDF membranes. The membranes were probed with N antibody. beta-actin was used as the internal reference.
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- Fig. 4 Pbp1 polyQ expansions inhibit Ty1 retromobility at the post-translational level. a Effects of Pbp1-polyQ expansion on Ty1his3AI retromobility (Mean +- SD; n = 5 independent cultures; Mann-Whitney U -test). b Semi-quantitative PCR products reflecting Ty1 integration upstream of 12 tRNA GLY loci. TEL1 amplification serves as control. c ChIP examining the localization of RNA Pol II at TyB using an alpha-RNA Pol II (phosphorylated serine 5) antibody (One-way ANOVA and Dunnett's post hoc). d Effects of Pbp1-polyQ expansion on the levels of Ty1 mRNA in RT-qPCR (One-way ANOVA and Dunnett's post hoc). e Confirmation of ATXN2-polyQ expression in transfected HEK293T cells by western blotting. f Effects of ATXN2 polyQ expansion on the levels of retroelement RNAs as detected by RT-qPCR. Values are normalized to GAPDH and statistics are relative to 23Q (Student's t -test). g Pbp1 polyQ forms exhibit an increased interaction with Trf4 ( n = 2). TAP-tagged Pbp1 proteins with indicated glutamine features were subjected to anti-TAP pulldowns followed by immunoblotting for endogenous Trf4, TAP tag or Actin control. h , i Interactions between Pbp1 forms or Trf4 with Ty1 mRNA. Presented are levels of Ty1 mRNA immunoprecipitated relative to input, as detected by RT-qPCR. Values are normalized to pull-down in untagged ( h ) or trf4 cells ( i ). j Effects of Pbp1-polyQ expansion on Ty1 mRNA levels in the presence/absence of TRF4 as detected by RT-qPCR (One
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- Figure 4 Binding and co-elution of ActM and PfnM. One potential binding partner was immobilized, the other added in solution. Protein blots and immunodetection of final wash (W) and eluate (E) are shown; employed antibody is indicated at the bottom. Molecular weights are 39 kDa for ActM and 17 kDa for PfnM.
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- Figure 1 ActM-GFP expression in E.coli. ActM-GFP adopts a variety of shapes and apparently is not freely diffusible in E.coli . All images show the GFP-channel, except for A2 and A3 which display the transmission channel and an overlay of transmission and GFP, respectively. Image F shows anti-actin/TRITC immunofluorescence of untagged ActM expressed in E.coli . Scale bars: 2 um.
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- Figure 5. Mg 2+ counters human R-loop accumulation by relying on RNaseH1 and the TRPM7 Mg 2+ transporter. ( A ) Anti-RNA-DNA hybrid immunofluorescence indicates that 10 mM Mg 2+ supplementation significantly decreases nuclear R-loop buildup in shATXN2 HeLa cells as shown in the scatter plot to better represent cell population data (N = 150 per condition; Mean +- Quartiles; **** P < 0.0001 for the Mann-Whitney test). ( B ) Representative anti-ATXN2 immunofluorescence images from cells transiently transfected with siCtl. or siATXN2 in the presence or absence of 10 mM Mg 2+ supplementation. Note how Mg 2+ supplementation does not alter ATXN2 levels or localization. ( C ) Anti-gammaH2AX immunofluorescence imaging and quantitation indicate that 10 mM Mg 2+ supplementation counters gammaH2AX accumulation in shATXN2 HeLa cells (N = 150 per condition; Mean +- Quartiles; **** P < 0.0001 for the Mann-Whitney test). ( D ) Anti-RNA-DNA hybrid immunofluorescence reveals that transient knockdown of RNaseH1, but not PIF1, abolishes the R-loop-suppressing effect of 10 mM Mg 2+ supplementation in shATXN2 HeLa cells (N = 150 per condition; Mean +- Quartiles; **** P < 0.0001, *** P < 0.001 for the Mann-Whitney test; n.s. not statistically significant). ( E ) Immunoblots showing transient knockdown of RNaseH1 in ATXN2-deficient HeLa cells. Anti-beta-Actin immunoblotting served as loading control. ( F ) Anti-RNA-DNA hybrid immunofluorescence reveals that transient knockdown of TRPM7, but not MAGT
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- Figure 6 Expression of alpha smooth muscle actin (alpha-SMA) by immunohistochemical staining (x100). ( A ) Photomicrograph of liver section of control rats showing minimal immunostaining for alpha -SMA; ( B ) Photomicrograph of liver section of CCl 4 intoxicated rats showing extensive alpha -SMA expression of as shown by the intense brown staining; ( C ) Photomicrograph of liver section of (CCl 4 /Silymarin) treated rats showing limited alpha -SMA expression; ( D ) Photomicrograph of liver section of rats concurrently treated with CCl 4 (1 mL/kg) twice a week and ETN (100 mg/kg) three times per week, showing limited alpha -SMA expression; ( E ) Photomicrograph of liver section of rats simultaneously treated with CCl 4 (1 mL/kg) twice a week and STN (100 mg/Kg) three times per week, showing minimal alpha -SMA expression; ( F ) A graphical representation of the alpha -SMA expression as optical density (O.D) for the liver sections from different groups, where a or b express the significant difference from control or CCl 4 group respectively at p < 0.05 using ANOVA followed by Tukey-Kramer as a post-hoc test.
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- Figure 1 Miro1-R272Q does not affect mitochondrial mass, but reduces mitochondrial movement. ( A ) Representative western blot images of Miro1 protein and the mitochondrial marker proteins Tom20 and Hsp60 in iPSC-derived neurons. Corresponding densitometries of western blot analyses normalized to beta-actin are shown at the right. Data indicated as mean +- SEM ( n = 3). ( B ) iPSC-derived neurons were fixed and stained against Tom20. Images were obtained using a 63x objective; scale bars indicate 10 mum. ( C ) Quantification of mitochondrial area per cell from the Tom20 signal from images shown in B. Data indicated as mean +- SEM. Significance calculated by Mann-Whitney test ( n = 4). ( D ) Analysis of maximum and mean speed per mitochondria from iPSC-derived neurons stained with MitoTracker Green FM. Data indicated as mean +- SEM. Significance calculated by Mann-Whitney test; * * * P < 0.001; ( n = 5).
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- Figure 5 Miro1-R272Q leads to alterations of autophagy flux and Rab9 protein levels. ( A ) Representative western blot image of p62 and Rab9 proteins in iPSC-derived neurons after bafilomycin A1 treatment. ( B) Densitometry of p62 western blot analysis normalized to beta-actin. Data indicated as mean +- SEM. Significance calculated by Mann-Whitney test; * P < 0.05; ( n = 3). ( C ) Densitometry of Rab9 western blot analysis normalized to beta-actin. Data indicated as mean +- SEM. Significance calculated by Mann-Whitney test; * P < 0.05; ( n = 3).
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- Figure 7 The total level of DICER, N-cadherin and E-cadherin proteins in A2780, A2780cis, SK-OV-3, and OVCAR-3 cell lines-Immunoblots. A2780, A2780cis, SK-OV-3, OVCAR3 ovarian cancer cell lines were cultured, harvested, and lysed with RIPA buffer. This Figure contains representative immunoblots of DICER, N-cadherin, E-cadherin, and beta-actin proteins level, which were obtained with the Immunoblotting-ECL method.
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- Figure 3. Stress-induced suppression of i NKT cell responses is mediated by glucocorticoids, not by SNS signals (A) WT B6 mice (n = 3 or 4 per group) were injected i.p. with OHDA or Veh 6 days before they were restrained. Control cohorts were left undisturbed. Stressed and control animals were injected with alphaGC and bled at the indicated time points. Circulating IL-4 and IFN-gamma levels were measured by ELISA. (B) Protein extracts from flash-frozen spleen and brain samples from mice in (A) were loaded onto the same SDS-polyacrylamide gel and examined for their tyrosine hydroxylase (TH) content by western blotting. Staining for beta-actin was used to ensure equal protein loading. Summary data illustrate normalized TH levels after densitometry analyses. (C) Separate cohorts that had been restrained for 12 h or left undisturbed were bled for serum CS quantification by ELISA. (D and E) Mice were injected i.p. with metyrapone (D) or RU486 (E) 1 h before they were restrained for 12 h (or not) followed by an i.p. injection of alphaGC. Box-and-whisker plots show IL-4 and IFN-gamma levels at 2- and 12-h time points post-alphaGC administration, respectively, with each symbol representing an individual mouse. Statistical comparisons were made by two-way ANOVA with Dunnett''s correction (A), unpaired Student''s t test (C), or one-way ANOVA with Tukey''s correction (D and E). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; NS, not significant.
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- Figure 10 Effects of a 6 h treatment with J3955 (0.75, 1.5, and 3 muM) on Cdk1 phosphorylation in HepG2 cells. After the treatment, cells were collected and the proteins were isolated for Western blot analysis as described in ''''Materials and methods''''. The panel shows a representative Western blot and densitometric analysis. The values represent the ratio between phospho-Cdk1 and total Cdk1, both previously normalized for the corresponding beta-actin. Values are expressed as the mean +- S.D. of three separate experiments with similar results. Different lowercase letters on the top of each histogram indicate statistical ( p < 0.05) differences among the tested samples, as measured by one-way ANOVA followed by the Tuckey test. The letter ""a"" marks the highest value. Bars not sharing the same letter were significantly different.
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- 10.1371/journal.pgen.1008755.g001 Fig 1 Okazaki fragments increase in length when Pol alpha is limiting. (A). Western blot against 13xMyc-tagged Pol1 from S. cerevisiae from a wild-type or GDPOL1 strain, as indicated, shifted to YPD or YEP + 3% raffinose (hereafter, media) supplemented with various concentrations of galactose. The lower band indicated by an asterisk is a degradation product resulting from degron-tagging. (B). Schematic of experimental workflow for Okazaki fragment analysis and sequencing (also see methods ). (C, D). Alkaline agarose gel analysis of end-labeled Okazaki fragments from a wild type (C) or GDPOL1 (D) strain, shifted to YPD or media supplemented with galactose as indicated. (E). Southern blot using a whole genome probe, on Okazaki fragments from a wild type or GDPOL1 strain shifted to media with the indicated sugar concentrations. (F, G). Distribution of Okazaki fragment 5' ends around consensus nucleosome dyads [] for a wild-type (F) or GDPOL1 (G) strain shifted to media containing the indicated concentration of galactose. Data for Okazaki fragment 3' ends in GDPOL1 are in S1F Fig .
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- Fig. 3 CoCl 2 induced the mRNA and protein expression of HIF-1 alpha to construct a hypoxic microenvironment. CoCl 2 significantly induced the mRNA ( A ) and protein ( B ) and ( C ) expression of HIF-1alpha in a dose-dependent manner. Cal-27 and SCC-15 cells were co-cultured with different concentrations of CoCl2 for 24 h, then the mRNA and protein expression levels of HIF-1alpha were analyzed by qRT-PCR and Western blot, which were normalized to those of beta-actin. Data were expressed as the mean +- SEM from three independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001 vs 50 muM CoCl 2 ; # p < 0.05, ## p < 0.01, ### p < 0.001 vs. 50 muM CoCl 2 .
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- Fig. 4 Hyperthermia and hypoxia-induced the expression of autophagy hallmark proteins in OSCC cells. Cal-27 and SCC-15 cells were cultured in hypoxia conditions and were exposed to 42 degC for 1 h, followed by recovery at 37 degC for 24 h. The protein and mRNA levels of HIF-1alpha, BNIP3, Beclin1, LC3-II, and p62 were analyzed by qRT-PCR and western blot and normalized to those of beta-actin. Data were expressed as the mean +- SEM from three independent experiments. a: Con; b: HT; c: Hy; d: HT + Hy. Con control, HT hyperthermia, Hy hypoxia, HT + Hy hyperthermia+hypoxia. * p < 0.05 vs. Con group; # p < 0.05 vs. HT group; & p < 0.05 vs. Hy group; no: no significant difference ( p > 0.05).
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- Fig. 5 The addition of YC-1 and 3-MA inhibited HT-induced autophagy in hypoxia and starvation tumor microenvironment, both of which involved downregulation of the HIF-1alpha/BNIP3 /Beclin1 signaling pathway. The mRNA and protein expression of HIF-1alpha, BNIP3, Beclin1, LC3-II, and p62 in OSCC cells were measured by Western blot and qRT-PCR, which were normalized to beta-actin. The results were presented as the mean +- standard deviation of three independent experiments. d: HT + Hy; e: HT + Hy+3-MA; f: HT + Hy+YC-1; g: HT + Hy+YC-1 + 3-MA. HT: hyperthermia; Hy: hypoxia; YC-1: 3-(5'-hydroxymethyl-2'-furyl)-1-benzylindazole; 3-MA: 3-Methyladenine. * p < 0.05 vs. HT + Hy group; # p < 0.05 vs. HT + Hy+3-MA group; & p < 0.05 vs. HT + Hy+YC-1 group; ns no significant difference ( p > 0.05).
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- Fig. 5. Y705 phosphorylation is needed for the correct localisation of STAT3 in the mitochondrion. (A) IF with anti-STAT3 and anti-ATAD3 antibodies on ESCs transiently transfected with either mStat3 , mStat3 Y705F or MLS_mStat3_NES Y705F . Arrowheads indicate the colocalisation of ATAD and STAT3. (B) Western blot of total STAT3 in ESC extracts; beta-actin was used as a loading control. (C) Western blot of mitochondrial STAT3 from ESC mitochondrial extracts; VDAC1 was used as a mitochondrial loading control, Lamin was used as a control for nuclear contamination. (D) Representative pictures of DAB IHC on ESCs acquired with TEM; positive signal is black and negative is white. Cristae are positive in Stat3 WT and MLS_Stat3_NES transfected cells. m, mitochondria; n, nucleus. Scale bar: 200 mum (A).
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- Figure 1 Loss of Bpnt2 impairs glycosaminoglycan sulfation but does not alter the PAP level. A , illustration of intracellular sulfation pathways, wherein BPNT2 hydrolyzes PAP, a by-product of sulfation, to AMP. B , absent expression of Bpnt2 mRNA (*** p = 0.0001) in Bpnt2-KO MEFs as determined by quantitative PCR and ( C ) absent BPNT2 protein in Bpnt2-KO MEFs, as determined by Western blot. D , Bpnt2-KO MEFs exhibit decreased levels of both intracellular ( left , **** p < 0.0001) and ( E ) secreted ( right , ** p = 0.0034) sulfated glycosaminoglycans, as determined by the DMMB assay. F , Bpnt2-KO MEFs do not show changes in the PAP level relative to WT cells. The bars show the mean +- SEM. Significance analyses are the results of unpaired Student's t test (two-sided). APS, adenosine phosphosulfate; BPNT1, bisphosphate nucleotidase 1; BPNT2, bisphosphate nucleotidase 2; cSULTs, cytosolic sulfotransferases; DMMB, dimethylmethylene blue; gSULTs, Golgi-resident sulfotransferases; MEFs, mouse embryonic fibroblasts; PAP, 3'-phosphoadenosine-5'-phosphate; PAPS, phosphoadenosine-phosphosulfate; PAPSS, PAPS synthase; PAPST, PAPS transporter.
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- Figure 3 Generation of mutant Bpnt2 MEF lines. A , blot for BPNT2 and actin on protein extracted from Bpnt2 MEF lines; arrows in D175N lane denote 2 bands, representing singly and doubly glycosylated protein. B , blot of proteins extracted from MEF lines, treated with PNGaseF to remove N-glycosyl groups; PNGaseF removes both glycosyl groups, resulting in a single band for BPNT2-D175N. Note that all bands shift downward with PNGaseF, as native murine BPNT2 has one N-glycosylation site at N257. C , a selection of Bpnt2 sequence from mouse and human Bpnt2 . In both, the D175N/D177N mutation results in the generation of an N-glycosyl consensus sequence. BPNT2, bisphosphate nucleotidase 2; MEF, mouse embryonic fibroblast.
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- Figure 1. Expression levels of IL-21 in OSCC tissues. (A) mRNA expression levels of IL-21 in OSCC tissues and adjacent normal tissues were detected using reverse transcription-quantitative PCR. (B) Protein expression levels of IL-21 in OSCC tissues and adjacent normal tissues were detected using western blotting. (C) Semi-quantification of the western blotting data presented in part B. Expression levels of IL-21 were normalized to beta-actin. The data are presented as the mean +- SEM from three independent experiments. **P
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- Figure 2. Adenoviral-mediated overexpression of IL-21 in CAL-27 cells. (A) Reverse transcription-quantitative PCR or (B) western blotting was used to analyze the mRNA or protein expression levels, respectively, of IL-21 in CAL-27 cells infected with Ad- or Ad-IL-21. (C) Semi-quantification of the western blotting data presented in part B. Expression levels of IL-21 were normalized to beta-actin. The data were expressed as the mean +- SEM from three independent experiments. *P
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- Figure 6 circCOL1A/miR-145/RABL3 axis regulates the proliferation, migration, and invasion of gastric cancer. (a) Western blot results of RABL3 in AGS cells. (b) Western blot results of RABL3 in MKN-45 cells. (c) CCK8 assay was used in AGS NC and si-circCOL1A1 cells. AGS NC and si-circCOL1A1 cells were rescued by transfection of the miR-145 inhibitor or RABL3 overexpression plasmids. (d) CCK8 assay was used in the MKN-45 vector and circCOL1A1 overexpression cells. MKN-45 vector and circCOL1A1 overexpression cells were then transfected with miR-145 mimics or RABL3 siRNA. (e) Migration assay was used in AGS NC and si-circCOL1A1 cells with indicated transfections. (f) Migration assay was used in the MKN-45 vector and circCOL1A1 overexpression cells with indicated transfections. (g) Invasion assay was used in AGS NC and si-circCOL1A1 cells with indicated transfections. (h) Invasion assay was used in the MKN-45 vector and circCOL1A1 overexpression cells with indicated transfections.
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- FIG. 4. Mutant Miro1 protein leads to reduction of mitochondrial mass. (A) Representative Western blot of Tom20 in immortalized fibroblasts. (B) Quantification of Tom20 protein level in immortalized fibroblasts from (A) . Significance tested with the Wilcoxon test ( n = 20). (C) Representative Western blot image of Hsp60 protein in immortalized fibroblasts. (D) Quantification of Hsp60 normalized to beta-actin from (C) . Significance assessed using the Mann-Whitney test ( n = 4). (E) Representative Western blot image of Miro1 protein in immortalized fibroblasts. (F) Quantification of Western blot analysis of Miro1 protein levels from (E) . Significance determined using the Wilcoxon test ( n = 5). (G) Representative Western blot image of Tom20 and (I) MnSOD proteins in M17 cells with stable knockdown of endogenous RHOT1 and transiently overexpression of Miro1 variants. (-) Indicates M17 cells without knockdown of endogenous RHOT1 . (+) Indicates knockdown of endogenous RHOT1 by stable transfection with the RHOT1 -targeting miRNA-2471 ( RHOT1 miRNA). M17 cells were transfected with Miro1-WT/myc (in pRK5-myc vector), Miro1-WT/V5, Miro1-R272Q, or Miro1-R450C (in pcDNA3.1/V5-HisA vector). (H) Quantification of Tom20 protein levels from (G) ( n = 3). (J) Quantification of MnSOD protein levels from (I) ( n = 3). All data indicated as mean +- SEM. * p
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- FIG. 5. Mitochondrial turnover is increased in Miro1-mutant fibroblasts. (A) Representative Western blot image of Miro1 in immortalized fibroblasts treated with 10 mu M MG132 for 24 h or with 10 n M bafilomycinA 1 for 48 h. (B) Quantification of Miro1 protein level from Western blots displayed in (A) . Significance assessed with the Wilcoxon test ( n = 3-7). (C) Western blot image for Tom20 protein in immortalized fibroblasts treated with 10 n M bafilomycinA 1 for 48 h or with 10 mu M MG132 for 24 h, respectively. (D) Quantification of Tom20 protein levels from Western blot analysis shown in (C) . Significance assessed using the Wilcoxon test ( n = 5). (E) Representative Western blot image of Parkin protein. Left panel shows Parkin bands in SH-SY5Y cells overexpressing Parkin. Right panel shows endogenous Parkin in immortalized fibroblasts treated with 10 mu M FCCP for 14 h. (F) Quantification of Parkin protein levels normalized to beta-actin from (E) . Significance calculated by the Mann-Whitney test ( n = 5). All data indicated as mean +- SEM. * p
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- FIG. 6. LC3-dependent autophagy is affected in Miro1-mutant fibroblasts. (A) Representative Western blot image of immortalized fibroblasts treated with 10 n M bafilomycinA 1 for 3, 6, or 24 h. (B) Densitometry analysis of Western blot for LC3-II normalized to beta-actin ( upper graph ) and the ratio of LC3-II/LC3-I protein levels ( lower graph ) from (A) . Significance calculated by the Friedman test ( n = 3). (C) Immortalized fibroblasts were transfected with mito-DsRed and labeled with 18:1 NBD-PS. Then, cells were starved in medium without FBS for 2 h for subsequent live cell imaging, using a 63 x objective. Cell selection shows microscopy images with mito-DsRed-labeled mitochondria ( red ) and 18:1 NBD-PS ( green ). Cells were manually selected ( dashed pink outline ) to ensure that only 18:1 NBD-PS-labeled autophagosomes in the cytosol were analyzed. The colocalization panel shows the selected cells from the data analysis. White squares indicate the regions, which were shown in the zoom panel . Autophagosomes were identified as green particles, which are not colocalizing with the mito-DsRed signal ( white arrows ). (D) Quantification of autophagosome formation from images shown in (C) . Significance calculated by the Wilcoxon test ( n = 3). (E) Colocalization of mitochondria and LC3 pixel from (F) were quantified and normalized to cell number. Significance assessed using the Mann-Whitney test ( n = 3, with ~30 cells per cell line). (F) Immortalized fibroblasts were trans
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- Figure 5. Ssd1 affects mRNA localization. ( A ) Representative Western blot showing Ssd1-GFP and markers of mitochondria, ER, and vacuole as detected in organelle-depleted and organelle-enriched fractions from SSD1-GFP or untagged-Ssd1 strains (see Methods). Ssd1 fragments migrating below the expected top band emerge during the fractionation incubations. ( B ) Representative smFISH showing bud-localized M MR1 transcript in wild-type and ssd1Delta aneuploids. ( C ) Quantification of percent buds lacking MMR1 from smFISH (see Materials and methods). ( D ) Percent of cells lacking Rhodamine staining. Histograms represent average and SEM across biological triplicates; *, p
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- Fig. 4 pti-DIMs promote genome-stabilising clustering of Rad52 droplets. a - f Live-cell microscopy shows that the pti-DIM-compromising tub3Delta ( a , b ) lowers droplet velocity ( c ), increases droplet numbers ( d ) and decreases droplet size ( e ), while western blotting shows Rad53 hyper-phosphorylation ( f ). kar3 served as control. Quantifications represent the mean +- s.d. ; * P