Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [4]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-11571 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Actin Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with bovine, chicken, canine, equine, hamster, mouse, porcine, non-human primate, rat, rabbit and Xenopus, yeast and zebrafish based on sequence homology.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 2 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references OsNSUN2-Mediated 5-Methylcytosine mRNA Modification Enhances Rice Adaptation to High Temperature.
Tang Y, Gao CC, Gao Y, Yang Y, Shi B, Yu JL, Lyu C, Sun BF, Wang HL, Xu Y, Yang YG, Chong K
Developmental cell 2020 May 4;53(3):272-286.e7
Developmental cell 2020 May 4;53(3):272-286.e7
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ACTB/ACTC (arrow) using an Actin polyclonal antibody (Product # PA5-11571) in 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected with the ACTB/ACTC gene (Lane 2).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ACTB/ACTC (arrow) using an Actin polyclonal antibody (Product # PA5-11571) in 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected with the ACTB/ACTC gene (Lane 2).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis using an Actin polyclonal antibody (Product # PA5-11571) in HL-60 cell lysates (35 µg per lane).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ACTB/ACTC (arrow) using an Actin polyclonal antibody (Product # PA5-11571) in 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected with the ACTB/ACTC gene (Lane 2).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of formalin-fixed, paraffin-embedded human skeletal muscle tissue using an Actin polyclonal antibody (Product # PA5-11571), followed by HRP-conjugated secondary antibody and DAB staining.
