15361-1-AP

antibody from Invitrogen Antibodies

Targeting: ARRB1 ARR1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

15361-1-AP

Provider

Invitrogen Antibodies

Product name

beta Arrestin 1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: DVLGLTFRK DLFVANVQSF PPAPEDKKPL TRLQERLIKK LGEHAYPFTF EIPPNLPCSV TLQPGPEDTG KACGVDYEVK AFCAENLEEK IHKRNSVRLV IRKVQYAPER PGPQPTAETT RQFLMSDKPL HLEASLDKEI YYHGEPISVN VHVTNNTNKT VKKIKISVRQ YADICLFNTA QYKCPVAMEE ADDTVAPSST FCKVYTLTPF LANNREKRGL ALDGKLKHED TNLASSTLLR EGANREILGI IVSYKVKVKL VVSRGGLLGD LASSDVAVEL PFTLMHPKPK EEPPHREVPE NETPVDTNLI ELDTNDDDIV FEDFARQRLK GMKDDKEEEE DGTGSPQLNN R (69-418 aa encoded by BC003636)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.13 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

A549 cells were subjected to SDS PAGE followed by western blot with 15361-1-AP (ARRB1 antibody) at dilution of 1:700 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

U-937 cells were subjected to SDS PAGE followed by western blot with 15361-1-AP (ARRB1 antibody) at dilution of 1:700 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse lung tissue were subjected to SDS PAGE followed by western blot with 15361-1-AP (ARRB1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using ARRB1 antibody 15361-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using 15361-1-AP ( beta Arrestin 1 antibody at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using 15361-1-AP ( beta Arrestin 1 antibody at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-beta Arrestin 1 (IP:15361-1-AP, 4ug; Detection:15361-1-AP 1:500) with mouse lung tissue lysate 4000ug.