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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [5]
- Immunocytochemistry [1]
- Immunoprecipitation [2]
- Immunohistochemistry [1]
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- Product number
- GTX100205 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX100205, RRID:AB_1240559
- Product name
- Caveolin 1 antibody [N1N3]
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references Interaction of Caveolin-3 and HCN is involved in the pathogenesis of diabetic cystopathy.
Expression of CAVEOLIN 1 in uterine mesenchymal tumors: No relationship between malignancy and CAVEOLIN 1 expression.
Dong X, Song Q, Zhu J, Zhao J, Liu Q, Zhang T, Long Z, Li J, Wu C, Wang Q, Hu X, Damaser M, Li L
Scientific reports 2016 Apr 28;6:24844
Scientific reports 2016 Apr 28;6:24844
Expression of CAVEOLIN 1 in uterine mesenchymal tumors: No relationship between malignancy and CAVEOLIN 1 expression.
Hayashi T, Ichimura T, Yaegashi N, Shiozawa T, Konishi I
Biochemical and biophysical research communications 2015 Aug 7;463(4):982-7
Biochemical and biophysical research communications 2015 Aug 7;463(4):982-7
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Supportive validation
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- Experimental details
- Sample (30 ug of whole cell lysate) A: H1299 12% SDS PAGE GTX100205 diluted at 1:500
- Validation comment
- WB
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- GeneTex (provider)
- Main image
- Experimental details
- Caveolin 1 antibody detects Caveolin 1 protein by western blot analysis. Various whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with Caveolin 1 antibody (GTX100205) diluted at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Caveolin 1 antibody [N1N3] detects Caveolin 1 protein by western blot analysis. Mouse tissue extracts (50 ?g) was separated by 12% SDS-PAGE, and the membrane was blotted with Caveolin 1 antibody [N1N3] (GTX100205) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Wild-type (WT) and Caveolin 1 knockout (KO) HeLa cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with Caveolin 1 antibody [N1N3] (GTX100205) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Wild-type (WT) and Caveolin 1 knockout (KO) HeLa cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with Caveolin 1 antibody [N1N3] (GTX100205) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
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- Main image
- Experimental details
- Caveolin 1 antibody [N1N3] detects Caveolin 1 protein at membrane by immunofluorescent analysis. Sample: A431 cells were fixed in ice-cold MeOH for 5 min.Green: Caveolin 1 protein stained by Caveolin 1 antibody [N1N3] (GTX100205) diluted at 1:500.Blue: Hoechst 33343 staining.
Supportive validation
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- Experimental details
- Caveolin 1 antibody [N1N3] immunoprecipitates caveolin 1 protein in IP experiments.IP samples: A549 membrane extractA. 30 ?g A549 membrane extractB. Control with 4 ?g of preimmune Rabbit IgGC. Immunoprecipitation of caveolin 1 protein by 4 ?g Caveolin 1 antibody [N1N3] (GTX100205)15 % SDS-PAGEThe immunoprecipitated caveolin 1 protein was detected by Caveolin 1 antibody [N1N3] (GTX100205) diluted at 1:500.[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
- Validation comment
- IP
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of Caveolin 1 protein from A549 membrane extracts using 5 £gg of Caveolin 1 antibody [N1N3] (GTX100205).Western blot analysis was performed using Caveolin 1 antibody [N1N3] (GTX100205).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Supportive validation
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- Immunohistochemical microscopy analysis of paraffin-embedded lung carcinoma cell line xenograft tissue using Caveolin-1 antibody [N1N3] (GTX100205) (1:100).
