MAB2408

antibody from Abnova Corporation

Targeting: CAV1 CAV

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB2408

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#MAB2408, RRID:AB_10632971

Product name

CAV1 monoclonal antibody, clone 7C8

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against CAV1.

Isotype

IgG

Antibody clone number

7C8

Storage

Store at -20°C or -80°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Flotillin-1 facilitates toll-like receptor 3 signaling in human endothelial cells.

Fork C, Hitzel J, Nichols BJ, Tikkanen R, Brandes RP
Basic research in cardiology 2014;109(6):439

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Immunopurification and characterization of rat adipocyte caveolae suggest their dissociation from insulin signaling.

Souto RP, Vallega G, Wharton J, Vinten J, Tranum-Jensen J, Pilch PF
The Journal of biological chemistry 2003 May 16;278(20):18321-9

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of CAV1 in NIH/3T3 cell lysates (50 ug) using CAV1 monoclonal antibody, clone 7C8 (Cat # MAB2408). Lanes 1 and 2 : 1 : 4,000. Lanes 3 and 4 : 1 : 1,000. ECL : 5 minute exposure.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent imaging using CAV1 monoclonal antibody, clone 7C8 (Cat # MAB2408) at 1 : 200 dilution (ON incubation) on EaHy926 endothelial cell line showing a clear localization in lipid raft/membrane (Photo courtesy of Alberto Davalos, Yale School of Medicine).

Validation comment

Immunofluorescence