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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [22]
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- Validations
- Western blot [1]
- Flow cytometry [3]
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- Product number
- NB100-615 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-615, RRID:AB_10003431
- Product name
- Mouse Monoclonal Caveolin-1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified. This is specific for caveolin alpha and beta proteins.
- Reactivity
- Human, Mouse, Rat, Sheep
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1.0 mg/ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Fungal allergen-induced IL-33 secretion involves cholesterol-dependent, VDAC-1-mediated ATP release from the airway epithelium.
Retinal VEGFA maintains the ultrastructure and function of choriocapillaris by preserving the endothelial PLVAP.
Expression of Stromal Caveolin- 1 May Be a Predictor for Aggressive Behaviour of Breast Cancer.
Hyperthermia and associated changes in membrane fluidity potentiate P2X7 activation to promote tumor cell death.
A preliminary study on the interaction between Asn-Gly-Arg (NGR)-modified multifunctional nanoparticles and vascular epithelial cells.
Tissue damage negatively regulates LPS-induced macrophage necroptosis.
The evaluation of the caveolin-1 and AT-rich interactive domain 1 alpha expressions in uterine smooth muscle tumors.
SMN affects membrane remodelling and anchoring of the protein synthesis machinery.
Basigin can be a therapeutic target to restore the retinal vascular barrier function in the mouse model of diabetic retinopathy.
Increases in endothelial caveolin-1 and cavins correlate with cirrhosis progression.
ATP stimulates pannexin 1 internalization to endosomal compartments.
Phosphodiesterase 2A localized in the spinal cord contributes to inflammatory pain processing.
Enterohemorrhagic Escherichia coli hemolysin employs outer membrane vesicles to target mitochondria and cause endothelial and epithelial apoptosis.
Caveolin-1--a novel interacting partner of organic cation/carnitine transporter (Octn2): effect of protein kinase C on this interaction in rat astrocytes.
Effects of gravitational mechanical unloading in endothelial cells: association between caveolins, inflammation and adhesion molecules.
Characterization and identification of PARM-1 as a new potential oncogene.
AMP kinase activation improves angiogenesis in pulmonary artery endothelial cells with in utero pulmonary hypertension.
Matrix metalloproteinase-9 in homocysteine-induced intestinal microvascular endothelial paracellular and transcellular permeability.
Functional heterogeneity of breast fibroblasts is defined by a prostaglandin secretory phenotype that promotes expansion of cancer-stem like cells.
Quantitative proteomics of caveolin-1-regulated proteins: characterization of polymerase i and transcript release factor/CAVIN-1 IN endothelial cells.
Akt regulates L-type Ca2+ channel activity by modulating Cavalpha1 protein stability.
Immunopurification and characterization of rat adipocyte caveolae suggest their dissociation from insulin signaling.
Srisomboon Y, Squillace DL, Maniak PJ, Kita H, O'Grady SM
The Journal of physiology 2020 May;598(10):1829-1845
The Journal of physiology 2020 May;598(10):1829-1845
Retinal VEGFA maintains the ultrastructure and function of choriocapillaris by preserving the endothelial PLVAP.
Kim SA, Kim SJ, Choi YA, Yoon HJ, Kim A, Lee J
Biochemical and biophysical research communications 2020 Jan 29;522(1):240-246
Biochemical and biophysical research communications 2020 Jan 29;522(1):240-246
Expression of Stromal Caveolin- 1 May Be a Predictor for Aggressive Behaviour of Breast Cancer.
Eliyatkin N, Aktas S, Diniz G, Ozgur HH, Ekin ZY, Kupelioglu A
Pathology oncology research : POR 2018 Jan;24(1):59-65
Pathology oncology research : POR 2018 Jan;24(1):59-65
Hyperthermia and associated changes in membrane fluidity potentiate P2X7 activation to promote tumor cell death.
de Andrade Mello P, Bian S, Savio LEB, Zhang H, Zhang J, Junger W, Wink MR, Lenz G, Buffon A, Wu Y, Robson SC
Oncotarget 2017 Sep 15;8(40):67254-67268
Oncotarget 2017 Sep 15;8(40):67254-67268
A preliminary study on the interaction between Asn-Gly-Arg (NGR)-modified multifunctional nanoparticles and vascular epithelial cells.
Liu C, Liu T, Yu X, Gu Y
Acta pharmaceutica Sinica. B 2017 May;7(3):361-372
Acta pharmaceutica Sinica. B 2017 May;7(3):361-372
Tissue damage negatively regulates LPS-induced macrophage necroptosis.
Li Z, Scott MJ, Fan EK, Li Y, Liu J, Xiao G, Li S, Billiar TR, Wilson MA, Jiang Y, Fan J
Cell death and differentiation 2016 Sep 1;23(9):1428-47
Cell death and differentiation 2016 Sep 1;23(9):1428-47
The evaluation of the caveolin-1 and AT-rich interactive domain 1 alpha expressions in uterine smooth muscle tumors.
Ayaz D, Diniz G, Kahraman DS, Sayhan S, Uncel M, Karadeniz T, Sanci M
Indian journal of pathology & microbiology 2016 Jul-Sep;59(3):301-4
Indian journal of pathology & microbiology 2016 Jul-Sep;59(3):301-4
SMN affects membrane remodelling and anchoring of the protein synthesis machinery.
Gabanella F, Pisani C, Borreca A, Farioli-Vecchioli S, Ciotti MT, Ingegnere T, Onori A, Ammassari-Teule M, Corbi N, Canu N, Monaco L, Passananti C, Di Certo MG
Journal of cell science 2016 Feb 15;129(4):804-16
Journal of cell science 2016 Feb 15;129(4):804-16
Basigin can be a therapeutic target to restore the retinal vascular barrier function in the mouse model of diabetic retinopathy.
Arima M, Cui D, Kimura T, Sonoda KH, Ishibashi T, Matsuda S, Ikeda E
Scientific reports 2016 Dec 5;6:38445
Scientific reports 2016 Dec 5;6:38445
Increases in endothelial caveolin-1 and cavins correlate with cirrhosis progression.
Yokomori H, Ando W, Yoshimura K, Yamazaki H, Takahashi Y, Oda M
Micron (Oxford, England : 1993) 2015 Sep;76:52-61
Micron (Oxford, England : 1993) 2015 Sep;76:52-61
ATP stimulates pannexin 1 internalization to endosomal compartments.
Boyce AK, Kim MS, Wicki-Stordeur LE, Swayne LA
The Biochemical journal 2015 Sep 15;470(3):319-30
The Biochemical journal 2015 Sep 15;470(3):319-30
Phosphodiesterase 2A localized in the spinal cord contributes to inflammatory pain processing.
Kallenborn-Gerhardt W, Lu R, Bothe A, Thomas D, Schlaudraff J, Lorenz JE, Lippold N, Real CI, Ferreirós N, Geisslinger G, Del Turco D, Schmidtko A
Anesthesiology 2014 Aug;121(2):372-82
Anesthesiology 2014 Aug;121(2):372-82
Enterohemorrhagic Escherichia coli hemolysin employs outer membrane vesicles to target mitochondria and cause endothelial and epithelial apoptosis.
Bielaszewska M, Rüter C, Kunsmann L, Greune L, Bauwens A, Zhang W, Kuczius T, Kim KS, Mellmann A, Schmidt MA, Karch H
PLoS pathogens 2013;9(12):e1003797
PLoS pathogens 2013;9(12):e1003797
Caveolin-1--a novel interacting partner of organic cation/carnitine transporter (Octn2): effect of protein kinase C on this interaction in rat astrocytes.
Czeredys M, Samluk Ł, Michalec K, Tułodziecka K, Skowronek K, Nałęcz KA
PloS one 2013;8(12):e82105
PloS one 2013;8(12):e82105
Effects of gravitational mechanical unloading in endothelial cells: association between caveolins, inflammation and adhesion molecules.
Grenon SM, Jeanne M, Aguado-Zuniga J, Conte MS, Hughes-Fulford M
Scientific reports 2013;3:1494
Scientific reports 2013;3:1494
Characterization and identification of PARM-1 as a new potential oncogene.
Charfi C, Levros LC Jr, Edouard E, Rassart E
Molecular cancer 2013 Jul 31;12:84
Molecular cancer 2013 Jul 31;12:84
AMP kinase activation improves angiogenesis in pulmonary artery endothelial cells with in utero pulmonary hypertension.
Teng RJ, Du J, Afolayan AJ, Eis A, Shi Y, Konduri GG
American journal of physiology. Lung cellular and molecular physiology 2013 Jan 1;304(1):L29-42
American journal of physiology. Lung cellular and molecular physiology 2013 Jan 1;304(1):L29-42
Matrix metalloproteinase-9 in homocysteine-induced intestinal microvascular endothelial paracellular and transcellular permeability.
Munjal C, Tyagi N, Lominadze D, Tyagi SC
Journal of cellular biochemistry 2012 Apr;113(4):1159-69
Journal of cellular biochemistry 2012 Apr;113(4):1159-69
Functional heterogeneity of breast fibroblasts is defined by a prostaglandin secretory phenotype that promotes expansion of cancer-stem like cells.
Rudnick JA, Arendt LM, Klebba I, Hinds JW, Iyer V, Gupta PB, Naber SP, Kuperwasser C
PloS one 2011;6(9):e24605
PloS one 2011;6(9):e24605
Quantitative proteomics of caveolin-1-regulated proteins: characterization of polymerase i and transcript release factor/CAVIN-1 IN endothelial cells.
Dávalos A, Fernández-Hernando C, Sowa G, Derakhshan B, Lin MI, Lee JY, Zhao H, Luo R, Colangelo C, Sessa WC
Molecular & cellular proteomics : MCP 2010 Oct;9(10):2109-24
Molecular & cellular proteomics : MCP 2010 Oct;9(10):2109-24
Akt regulates L-type Ca2+ channel activity by modulating Cavalpha1 protein stability.
Catalucci D, Zhang DH, DeSantiago J, Aimond F, Barbara G, Chemin J, Bonci D, Picht E, Rusconi F, Dalton ND, Peterson KL, Richard S, Bers DM, Brown JH, Condorelli G
The Journal of cell biology 2009 Mar 23;184(6):923-33
The Journal of cell biology 2009 Mar 23;184(6):923-33
Immunopurification and characterization of rat adipocyte caveolae suggest their dissociation from insulin signaling.
Souto RP, Vallega G, Wharton J, Vinten J, Tranum-Jensen J, Pilch PF
The Journal of biological chemistry 2003 May 16;278(20):18321-9
The Journal of biological chemistry 2003 May 16;278(20):18321-9
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Caveolin-1 Antibody (7C8) [NB100-615] - Detection of caveolin in 3T3 cell lysates (50 ug). Lanes 1 and 2: 1:4000. Lanes 3 and 4: 1:1000. Detection by ECL: 5 minute exposure.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Caveolin-1 Antibody (7C8) [NB100-615] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using Caveolin 1 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Caveolin-1 Antibody (7C8) [NB100-615] - An intracellular stain was performed on HeLa cells with Caveolin-1 Antibody (7C8) NB100-615 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Caveolin-1 Antibody (7C8) [NB100-615] - An intracellular stain was performed on HeLa cells with Caveolin-1 Antibody (7C8) NB100-615APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to allophycocyanin.
