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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- MA5-27841 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MTPAP Monoclonal Antibody (1D3)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Positive Control: A549, H1299, HCT116, MCF-7
- Antibody clone number
- 1D3
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MTPAP in A) A549, B) H1299, C) HCT116, D) MCF-7 cells using 30 µg of protein. Samples were separated with 7.5% SDS-PAGE and incubated with MTPAP monoclonal antibody (Product # MA5-27841) using a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using MTPAP Monoclonal Antibody (1D3) (Product # MA5-27841). Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with MTPAP Monoclonal Antibody (1D3) (Product # MA5-27841) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of MTPAP in methanol-fixed HeLa cells using MTPAP Monoclonal Antibody (1D3) (Product # MA5-27841) at a dilution of 1:100.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Hepatoma, using mtPAP (Product # MA5-27841) antibody at 1:200 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
