Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
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Validation data
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- Product number
- H00001415-M02 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00001415-M02, RRID:AB_10966526
- Product name
- CRYBB2 monoclonal antibody (M02), clone 1F1
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full-length recombinant CRYBB2.
- Antigen sequence
MASDHQTQAGKPQSLNPKIIIFEQENFQGHSHELN
GPCPNLKETGVEKAGSVLVQAGPWVGYEQANCKGE
QFVFEKGEYPRWDSWTSSRRTDSLSSLRPIKVDSQ
EHKIILYENPNFTGKKMEIIDDDVPSFHAHGYQEK
VSSVRVQSGTWVGYQYPGYRGLQYLLEKGDYKDSS
DFGAPHPQVQSVRRIRDMQWHQRGAFHPSN- Isotype
- IgG
- Antibody clone number
- 1F1
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of CRYBB2 expression in transfected 293T cell line by CRYBB2 monoclonal antibody (M02), clone 1F1.Lane 1: CRYBB2 transfected lysate (Predicted MW: 23.4 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged CRYBB2 is 3 ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol