AP1984i

antibody from Abgent

Targeting: JUN AP-1, c-Jun

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP1984i

Provider

Abgent

Proper citation

Abgent Cat#AP1984i, RRID:AB_10664860

Product name

JUN Antibody (C-term)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Antibody clone number

RB25952

Vial size

400 µl

Storage

Store at 2 to 8°C.Antibody is stable for 34 months.

Western blot

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

JUN Antibody (C-term) (Cat. #AP1984i) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the JUN antibody detected the JUN protein (arrow).

Primary Ab dilution

1:1000

Immunohistochemistry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

JUN Antibody (C-term) (Cat. #AP1984I) immunohistochemistry analysis in formalin fixed and paraffin embedded human uterus tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of JUN Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Primary Ab dilution

1:50~100

Flow cytometry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

JUN Antibody (C-term) (Cat. #AP1984i) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Primary Ab dilution

1:10~50