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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Western blot [2]
- Other assay [1]
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- Product number
- 459300 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- COX3 Monoclonal Antibody (DA5BC4)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Positive control: Isolated mitochondria from Human , Rat and Mouse hearts.
- Antibody clone number
- DA5BC4
- Concentration
- 1 mg/mL
Submitted references Distinct Roles of Mitochondrial HIGD1A and HIGD2A in Respiratory Complex and Supercomplex Biogenesis.
MrpL35, a mitospecific component of mitoribosomes, plays a key role in cytochrome c oxidase assembly.
Mutational Analysis of the QRRQ Motif in the Yeast Hig1 Type 2 Protein Rcf1 Reveals a Regulatory Role for the Cytochrome c Oxidase Complex.
Timón-Gómez A, Garlich J, Stuart RA, Ugalde C, Barrientos A
Cell reports 2020 May 5;31(5):107607
Cell reports 2020 May 5;31(5):107607
MrpL35, a mitospecific component of mitoribosomes, plays a key role in cytochrome c oxidase assembly.
Box JM, Kaur J, Stuart RA
Molecular biology of the cell 2017 Nov 15;28(24):3489-3499
Molecular biology of the cell 2017 Nov 15;28(24):3489-3499
Mutational Analysis of the QRRQ Motif in the Yeast Hig1 Type 2 Protein Rcf1 Reveals a Regulatory Role for the Cytochrome c Oxidase Complex.
Garlich J, Strecker V, Wittig I, Stuart RA
The Journal of biological chemistry 2017 Mar 31;292(13):5216-5226
The Journal of biological chemistry 2017 Mar 31;292(13):5216-5226
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of COX3 in isolated heart mitochondria extracts using a COX3 Monoclonal Antibody (Product # 459300) at a concentration of 4 µg/mL. Lane 1 : Isolated mitochondria from Human heart at 5 µg, Lane 2 : Isolated mitochondria Rat heart at 10 µg, Lane 3 : Isolated mitochondria from Mouse heart at 10 µg. Predicted band size: 30 kDa.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of COX3 in isolated heart mitochondria extracts using a COX3 Monoclonal Antibody (Product # 459300) at a concentration of 4 µg/mL. Lane 1 : Isolated mitochondria from Human heart at 5 µg, Lane 2 : Isolated mitochondria Rat heart at 10 µg, Lane 3 : Isolated mitochondria from Mouse heart at 10 µg. Predicted band size: 30 kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
