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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [5]
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- Product number
- GTX112846 - Provider product page
- Provider
- GeneTex
- Product name
- CREB antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references Development of a computational promoter with highly efficient expression in tumors.
Immunocytochemistry and fluorescence imaging efficiently identify individual neurons with CRISPR/Cas9-mediated gene disruption in primary cortical cultures.
Subchronic Arsenic Exposure Induces Anxiety-Like Behaviors in Normal Mice and Enhances Depression-Like Behaviors in the Chemically Induced Mouse Model of Depression.
Ho SY, Chang BH, Chung CH, Lin YL, Chuang CH, Hsieh PJ, Huang WC, Tsai NM, Huang SC, Liu YK, Lo YC, Liao KW
BMC cancer 2018 Apr 27;18(1):480
BMC cancer 2018 Apr 27;18(1):480
Immunocytochemistry and fluorescence imaging efficiently identify individual neurons with CRISPR/Cas9-mediated gene disruption in primary cortical cultures.
Tsunematsu H, Uyeda A, Yamamoto N, Sugo N
BMC neuroscience 2017 Aug 1;18(1):55
BMC neuroscience 2017 Aug 1;18(1):55
Subchronic Arsenic Exposure Induces Anxiety-Like Behaviors in Normal Mice and Enhances Depression-Like Behaviors in the Chemically Induced Mouse Model of Depression.
Chang CY, Guo HR, Tsai WC, Yang KL, Lin LC, Cheng TJ, Chuu JJ
BioMed research international 2015;2015:159015
BioMed research international 2015;2015:159015
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- CREB antibody detects CREB protein by western blot analysis.A. 30 ?g 293T whole cell lysate/extractB. 30 ?g whole cell lysate/extract of human CREB1-transfected 293T cells10 % SDS-PAGECREB antibody (GTX112846) dilution: 1:5000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- CREB antibody detects CREB protein by western blot analysis. Jurkat whole cell extracts and nuclear extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CREB antibody (GTX112846) at a dilution of 1:1000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- CREB antibody detects CREB protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: CREB protein stained by CREB antibody (GTX112846) diluted at 1:500.Red: phalloidin, a cytoskeleton marker, diluted at 1:50.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- CREB antibody detects CREB protein at nucleus on mouse lung by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. CREB antibody (GTX112846) dilution: 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- CREB antibody detects CREB protein at nucleus on mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. CREB antibody (GTX112846) dilution: 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- CREB antibody detects CREB protein at nucleus on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. CREB antibody (GTX112846) dilution: 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- CREB antibody detects CREB protein at nucleus in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. CREB antibody (GTX112846) diluted at 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- CREB antibody detects CREB protein at nucleus in rat testis by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. CREB antibody (GTX112846) diluted at 1:500.
