12208-1-AP

antibody from Proteintech Group

Targeting: CREB1

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

12208-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#12208-1-AP, RRID:AB_2245417

Product name

CREB1 antibody

Antibody type

Polyclonal

Description

KD/KO validated CREB1 antibody (Cat. #12208-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat, monkey and has been validated for the following applications: FC, IF, IHC, IP, WB, ELISA.

Reactivity

Human, Mouse, Rat, Simian

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

CREB1 and ATF1 Negatively Regulate Glutathione Biosynthesis Sensitizing Cells to Oxidative Stress.

Zhao L, Xia W, Jiang P
Frontiers in cell and developmental biology 2021;9:698264

---

LncRNA H19 Upregulation Participates in the Response of Glioma Cells to Radiation.

Kuang Y, Bing Z, Jin X, Li Q
BioMed research international 2021;2021:1728352

---

GluN2A/ERK/CREB Signaling Pathway Involved in Electroacupuncture Regulating Hypothalamic-Pituitary-Adrenal Axis Hyperactivity.

Wang Y, Han J, Zhu J, Zhang M, Ju M, Du Y, Tian Z
Frontiers in neuroscience 2021;15:703044

---

Edaravone Alleviated Propofol-Induced Neurotoxicity in Developing Hippocampus by mBDNF/TrkB/PI3K Pathway.

Yang Y, Yi J, Pan M, Hu B, Duan H
Drug design, development and therapy 2021;15:1409-1422

---

A Potential Mechanism Underlying the Therapeutic Effects of Progesterone and Allopregnanolone on Ketamine-Induced Cognitive Deficits.

Cao T, Tang M, Jiang P, Zhang B, Wu X, Chen Q, Zeng C, Li N, Zhang S, Cai H
Frontiers in pharmacology 2021;12:612083

---

An Integrative Pharmacology-Based Strategy to Uncover the Mechanism of Xiong-Pi-Fang in Treating Coronary Heart Disease with Depression.

Zhang L, Zhang Y, Zhu M, Pei L, Deng F, Chen J, Zhang S, Cong Z, Du W, Xiao X
Frontiers in pharmacology 2021;12:590602

---

KDELR2 promotes breast cancer proliferation via HDAC3-mediated cell cycle progression.

Wei H, Ma W, Lu X, Liu H, Lin K, Wang Y, Ye Z, Sun L, Huang Z, Pan T, Zhou Z, Cheng EY, Zhang H, Gao P, Zhong X
Cancer communications (London, England) 2021 Sep;41(9):904-920

---

Adenylyl cyclase 3 regulates osteocyte mechanotransduction and primary cilium.

Duffy MP, Sup ME, Guo XE
Biochemical and biophysical research communications 2021 Oct 8;573:145-150

---

miR-221/222 suppression induced by activation of the cAMP/PKA/CREB1 pathway is required for cAMP-induced bidirectional differentiation of glioma cells.

Liu Q, Tian R, Yu P, Shu M
FEBS letters 2021 Nov;595(22):2829-2843

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Role of the BDNF/TrkB/CREB signaling pathway in the cytotoxicity of bisphenol S in SK-N-SH cells.

He QZ, Zhu BQ, Xu XN, Zeng HC
Journal of biochemical and molecular toxicology 2021 Jun;35(6):1-11

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Congenital hypothyroidism impairs spine growth of dentate granule cells by downregulation of CaMKIV.

Tang Q, Chen S, Wu H, Song H, Wang Y, Shi J, Wu Y
Cell death discovery 2021 Jun 14;7(1):143

---

A CREB1-miR-181a-5p loop regulates the pathophysiologic features of bone marrow stromal cells in fibrous dysplasia of bone.

Fu Y, Xin Z, Ling Z, Xie H, Xiao T, Shen X, Lin J, Xu L, Jiang H
Molecular medicine (Cambridge, Mass.) 2021 Jul 22;27(1):81

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Uncaria rhynchophylla ameliorates unpredictable chronic mild stress-induced depression in mice via activating 5-HT_1A receptor: Insights from transcriptomics.

Qiao YL, Zhou JJ, Liang JH, Deng XP, Zhang ZJ, Huang HL, Li S, Dai SF, Liu CQ, Luan ZL, Yu ZL, Sun CP, Ma XC
Phytomedicine : international journal of phytotherapy and phytopharmacology 2021 Jan;81:153436

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Rapid antidepressant-like effect of Fructus Aurantii depends on cAMP-response element binding protein/Brain-derived neurotrophic facto by mediating synaptic transmission.

Wu L, Zhang T, Chen K, Lu C, Liu XF, Zhou JL, Huang YK, Yan H, Chen Y, Zhang CJ, Li JF, Shi SQ, Ren P, Huang X
Phytotherapy research : PTR 2021 Jan;35(1):404-414

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Glycogen synthase kinase-3β inhibitor SB216763 promotes DNA repair in ischemic retinal neurons.

Zhang J, Lai ZP, Chen P, Ying Y, Zhuang J, Yu KM
Neural regeneration research 2021 Feb;16(2):394-400

---

Pan-cancer multi-omics analyses reveal crosstalk between the Hippo and immune signaling pathways in the tumor microenvironment.

Wang C, Zhu T, Zhao Z, Chen B, Chen T, Dong Q, Liu M, Zhuang S, Yang F, Liu Y, Yang M, Gu Y, Liang H
FEBS letters 2021 Dec 2;

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Duzhong Butiansu Prescription Improves Heat Stress-Induced Spermatogenic Dysfunction by Regulating Sperm Formation and Heat Stress Pathway.

Hou Y, Yuan P, Fu Y, Zhang Q, Wei Y, Gao L, Liu L, Wang X, Zheng X, Feng W
Evidence-based complementary and alternative medicine : eCAM 2020;2020:6723204

---

LncRNA BLACAT1/miR-519d-3p/CREB1 Axis Mediates Proliferation, Apoptosis, Migration, Invasion, and Drug-Resistance in Colorectal Cancer Progression.

Chen R, Zhou S, Chen J, Lin S, Ye F, Jiang P
Cancer management and research 2020;12:13137-13148

---

Homer1a Undergoes Bimodal Transcriptional Regulation by CREB and the Circadian Clock.

Sato S, Bunney BG, Vawter MP, Bunney WE, Sassone-Corsi P
Neuroscience 2020 May 10;434:161-170

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Bexarotene Improve Depression-Like Behaviour in Mice by Protecting Against Neuro-inflammation and Synaptic Damage.

Yuan C, Dai C, Li Z, Zheng L, Zhao M, Dong S
Neurochemical research 2020 Jul;45(7):1500-1509

---

LncRNA MALAT1 inhibits apoptosis of endometrial stromal cells through miR-126-5p-CREB1 axis by activating PI3K-AKT pathway.

Feng Y, Tan BZ
Molecular and cellular biochemistry 2020 Dec;475(1-2):185-194

---

Down-Regulated CUEDC2 Increases GDNF Expression by Stabilizing CREB Through Reducing Its Ubiquitination in Glioma.

Liu XF, Tang CX, Zhang L, Tong SY, Wang Y, Abdulrahman AA, Ji GQ, Gao Y, Gao DS, Zhang BL
Neurochemical research 2020 Dec;45(12):2915-2925

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Saikosaponin-d impedes hippocampal neurogenesis and causes cognitive deficits by inhibiting the survival of neural stem/progenitor cells via neurotrophin receptor signaling in mice.

Qin T, Yuan Z, Yu J, Fu X, Deng X, Fu Q, Ma Z, Ma S
Clinical and translational medicine 2020 Dec;10(8):e243

---

PTEN participates in airway remodeling of asthma by regulating CD38/Ca²⁺/CREB signaling.

Wu Y, Lu Y, Zou F, Fan X, Li X, Zhang H, Chen H, Sun X, Liu Y
Aging 2020 Aug 27;12(16):16326-16340

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The potential role of Krüppel-like factor 13 (Aj-klf13) in the intestine regeneration of sea cucumber Apostichopus japonicus.

Chen L, Yao F, Qin Y, Shao Y, Fang L, Yu X, Wang S, Hou L
Gene 2020 Apr 20;735:144407

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MicroRNA-23a-3p Inhibits Mucosal Melanoma Growth and Progression through Targeting Adenylate Cyclase 1 and Attenuating cAMP and MAPK Pathways.

Ma M, Dai J, Tang H, Xu T, Yu S, Si L, Cui C, Sheng X, Chi Z, Mao L, Wu X, Yang L, Yu H, Li S, Lian B, Tang B, Wang X, Yan X, Bai X, Zhou L, Kong Y, Guo J
Theranostics 2019;9(4):945-960

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CircACC1 Regulates Assembly and Activation of AMPK Complex under Metabolic Stress.

Li Q, Wang Y, Wu S, Zhou Z, Ding X, Shi R, Thorne RF, Zhang XD, Hu W, Wu M
Cell metabolism 2019 Jul 2;30(1):157-173.e7

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ZNF436 promotes tumor cell proliferation through transcriptional activation of BCL10 in glioma.

Shang Y, Li Y, Zhang Y, Wang J
Biochemical and biophysical research communications 2019 Aug 6;515(4):572-578

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Metformin Modulates High Glucose-Incubated Human Umbilical Vein Endothelial Cells Proliferation and Apoptosis Through AMPK/CREB/BDNF Pathway.

Han X, Wang B, Sun Y, Huang J, Wang X, Ma W, Zhu Y, Xu R, Jin H, Liu N
Frontiers in pharmacology 2018;9:1266

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Neurotoxicity, behavioral changes and gene-expression profile of mice exposed to SnS₂ nanoflowers.

Bai D, Li Q, Wang J, Zhao J, Deng X, Yuan L, Wu P
Toxicology research 2018 Nov 1;7(6):1267-1279

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Calretinin Participates in Regulating Steroidogenesis by PLC-Ca²⁺-PKC Pathway in Leydig Cells.

Xu W, Zhu Q, Liu S, Dai X, Zhang B, Gao C, Gao L, Liu J, Cui Y
Scientific reports 2018 May 9;8(1):7403

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Analysis of the oncogene BRAF mutation and the correlation of the expression of wild-type BRAF and CREB1 in endometriosis.

Lv X, Wang D, Ma Y, Long Z
International journal of molecular medicine 2018 Mar;41(3):1349-1356

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Effects of fluoride on synapse morphology and myelin damage in mouse hippocampus.

Niu R, Chen H, Manthari RK, Sun Z, Wang J, Zhang J, Wang J
Chemosphere 2018 Mar;194:628-633

---

Inhibition of Acid Sensing Ion Channel 3 Aggravates Seizures by Regulating NMDAR Function.

Cao Q, Xiao ZM, Wang X, Weng C, Ding M, Zhu F, Lu ZN
Neurochemical research 2018 Jun;43(6):1227-1241

---

A CREB1/miR-433 reciprocal feedback loop modulates proliferation and metastasis in colorectal cancer.

Yan L, You WQ, Sheng NQ, Gong JF, Hu LD, Tan GW, Chen HQ, Wang ZG
Aging 2018 Dec 6;10(12):3774-3793

---

Icariin Attenuates Synaptic and Cognitive Deficits in an Aβ_1-42-Induced Rat Model of Alzheimer's Disease.

Sheng C, Xu P, Zhou K, Deng D, Zhang C, Wang Z
BioMed research international 2017;2017:7464872

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Dysregulation of hepatic cAMP levels via altered Pde4b expression plays a critical role in alcohol-induced steatosis.

Avila DV, Barker DF, Zhang J, McClain CJ, Barve S, Gobejishvili L
The Journal of pathology 2016 Sep;240(1):96-107

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Epicatechin Plus Treadmill Exercise are Neuroprotective Against Moderate-stage Amyloid Precursor Protein/Presenilin 1 Mice.

Zhang Z, Wu H, Huang H
Pharmacognosy magazine 2016 May;12(Suppl 2):S139-46

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LncRNA-UCA1 enhances cell proliferation and 5-fluorouracil resistance in colorectal cancer by inhibiting miR-204-5p.

Bian Z, Jin L, Zhang J, Yin Y, Quan C, Hu Y, Feng Y, Liu H, Fei B, Mao Y, Zhou L, Qi X, Huang S, Hua D, Xing C, Huang Z
Scientific reports 2016 Apr 5;6:23892

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BDNF-dependent recycling facilitates TrkB translocation to postsynaptic density during LTP via a Rab11-dependent pathway.

Huang SH, Wang J, Sui WH, Chen B, Zhang XY, Yan J, Geng Z, Chen ZY
The Journal of neuroscience : the official journal of the Society for Neuroscience 2013 May 22;33(21):9214-30

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

The CREB1 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human CREB1. This antibody recognizes human, mouse, rat, monkey antigen. The CREB1 antibody has been validated for the following applications: ELISA, IP, WB, IF, IHC analysis.

Appendix

12208-1-AP.pdf

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Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunofluorescent analysis of HEK-293 cells using 12208-1-AP( CREB1 Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG

Sample type

cell line

Immunoprecipitation

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

IP Result of anti-CREB1 (IP:12208-1-AP, 3ug; Detection:12208-1-AP 1:600) with HEK-293 cells lysate 2000ug.

Sample type

cell line