Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- PA5-72501 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MT-ND5 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 2 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ND5 in the following samples: Lane 1: CCRF-CEM cell lysate, Lane 2: Jurkat cell lysate. 20 µg of protein per lane was used. Samples were first blocked with 5% NFDM/TBST, incubated with ND5 polyclonal antibody (Product # PA5-72501) using a dilution of 1:1000 followed by a goat anti-rabbit IgG H&L (HRP) secondary antibody with a dilution of 1:10,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of ND5 in paraffin-embedded human brain tissue. Samples were incubated with ND5 polyclonal antibody (Product # PA5-72501) followed by a peroxidase-conjugate secondary antibody and DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of ND5 in CEM cells (bottom histogram) compared to a negative control cell (top histogram). Samples were incubated with ND5 polyclonal antibody (Product # PA5-72501) followed by a FITC conjugated goat anti-rabbit secondary antibody.