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Western blot
Immunocytochemistry
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [3]
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- Product number
- LS-C368519 - Provider product page
- Provider
- LSBio
- Product name
- RPS6KB1 / P70S6K / S6K Antibody (phospho-Thr229) LS-C368519
- Antibody type
- Polyclonal
- Description
- Immunoaffinity purified
- Reactivity
- Human, Mouse, Rat, Bovine, Rabbit
- Host
- Rabbit
- Storage
- Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.
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Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot analysis of S6K1 (pT229) expression in HT1080 (A); LOVO (B); NIH3T3 (C) whole cell lysates.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Western blot analysis of S6K1 (pT229) expression in HT1080 (A); LOVO (B); NIH3T3 (C) whole cell lysates.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Western blot analysis of S6K1 (pT229) expression in HT1080 (A); LOVO (B); NIH3T3 (C) whole cell lysates.
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunohistochemical analysis of S6K1 (pT229) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of S6K1 (pT229) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of S6K1 (pT229) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
