Explore
Validate
Learn
Western blot
ELISA
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Immunocytochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- ALX-210-302-R100 - Provider product page
- Provider
- Enzo Life Sciences
- Proper citation
- Enzo Life Sciences Cat#ALX-210-302-R100, RRID:AB_2052175
- Product name
- PARP-1 polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Reactivity
- Human, Mouse, Bovine, Simian
- Host
- Rabbit
- Vial size
- 100 μl
- Storage
- +4°C
- Handling
- Avoid freeze/thaw cycles.
Submitted references Lysosomal calcium signalling regulates autophagy through calcineurin and TFEB.
NF-κB transcriptional activation by TNFα requires phospholipase C, extracellular signal-regulated kinase 2 and poly(ADP-ribose) polymerase-1.
The prevention of spontaneous apoptosis of follicular lymphoma B cells by a follicular dendritic cell line: involvement of caspase-3, caspase-8 and c-FLIP.
Medina DL, Di Paola S, Peluso I, Armani A, De Stefani D, Venditti R, Montefusco S, Scotto-Rosato A, Prezioso C, Forrester A, Settembre C, Wang W, Gao Q, Xu H, Sandri M, Rizzuto R, De Matteis MA, Ballabio A
Nature cell biology 2015 Mar;17(3):288-99
Nature cell biology 2015 Mar;17(3):288-99
NF-κB transcriptional activation by TNFα requires phospholipase C, extracellular signal-regulated kinase 2 and poly(ADP-ribose) polymerase-1.
Vuong B, Hogan-Cann AD, Alano CC, Stevenson M, Chan WY, Anderson CM, Swanson RA, Kauppinen TM
Journal of neuroinflammation 2015 Dec 4;12:229
Journal of neuroinflammation 2015 Dec 4;12:229
The prevention of spontaneous apoptosis of follicular lymphoma B cells by a follicular dendritic cell line: involvement of caspase-3, caspase-8 and c-FLIP.
Goval JJ, Thielen C, Bourguignon C, Greimers R, Dejardin E, Choi YS, Boniver J, de Leval L
Haematologica 2008 Aug;93(8):1169-77
Haematologica 2008 Aug;93(8):1169-77
No comments: Submit comment
Supportive validation
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Western blot using rabbit polyclonal anti PARP-1. Lane 1: recombinant human PARP-1 (ALX-201-053, 5 ng). Lane 2: Total HeLa cell extract. Lane 3: Total MEF PARP1+/+ cell extract. Lane 4: Total MEF PARP1-/- cell extract. Lane 5: Lysate (50 _g) from HeLa cells. Lane 6: Lysate (50 _g) from HeLa PARP-1sh cells. Lane 7: Lysate (50 _g) from HeLa cells treated for 8 hours with Doxorubicin 5 _g/ml. Lane 8: Lysate (50 _g) from HEK293 cells. Lane 9: Lysate (50 _g) from HEK293 cells. Lane 10: Lysate (50 _g) from HEK293 cells transfected with PARP-1 siRNA. Lane 11: Lysate (50 _g) from HEK293 cells transfected with control siRNA.
