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LearnTA500855
antibody from OriGene
Targeting: GRIPAP1
DKFZp434P0630, GRASP-1, GRASP1, KIAA1167, MPMGp800B12492Q3
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- TA500855 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA500855, RRID:AB_11126857
- Product name
- Anti-GRIPAP1 (GRASP1) mouse monoclonal antibody, clone OTI2C11 (formerly 2C11)
- Antibody type
- Monoclonal
- Description
- Anti-GRIPAP1 (GRASP1) mouse monoclonal antibody, clone OTI2C11 (formerly 2C11)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- GRIPAP1
- Isotype
- IgG
- Antibody clone number
- OTI2C11
- Vial size
- 100 µl
- Concentration
- 0.5 mg/ml
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GRIPAP1 (RC210936, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GRIPAP1.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10ug) from 5 different cell lines by using anti-GRIPAP1 monoclonal antibody.(1:200)
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-GRIPAP1 mouse monoclonal antibody (TA500855) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GRIPAP1(RC210936).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-GRIPAP1mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500855, Dilution 1:50)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either pCMV6-ENTRY GRIPAP1(RC210936)(Red) or empty vector control plasmid(Blue) were immunostained with anti-GRIPAP1 mouse monoclonal(TA500855), and then analyzed by flow cytometry.
- Validation comment
- FC
