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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- GTX113523 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX113523, RRID:AB_2037767
- Product name
- PP2A alpha antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
Submitted references IER family proteins are regulators of protein phosphatase PP2A and modulate the phosphorylation status of CDC25A.
Andrographolide induces vascular smooth muscle cell apoptosis through a SHP-1-PP2A-p38MAPK-p53 cascade.
Ueda T, Kohama Y, Sakurai H
Cellular signalling 2019 Mar;55:81-89
Cellular signalling 2019 Mar;55:81-89
Andrographolide induces vascular smooth muscle cell apoptosis through a SHP-1-PP2A-p38MAPK-p53 cascade.
Chen YY, Hsieh CY, Jayakumar T, Lin KH, Chou DS, Lu WJ, Hsu MJ, Sheu JR
Scientific reports 2014 Jul 10;4:5651
Scientific reports 2014 Jul 10;4:5651
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (50 ug of whole cell lysate) A: Mouse brain 10% SDS PAGE GTX113523 diluted at 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: Molt-4 (GTX27912) 10% SDS PAGE GTX113523 diluted at 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- PP2A alpha antibody detects PP2A alpha protein by western blot analysis. Whole cell extracts (30 £gg) was separated by 10% SDS-PAGE, and the membrane was blotted with PP2A alpha antibody (GTX113523) at a dilution of 1:1000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using PP2A alpha(GTX113523) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using PP2A(GTX113523) antibody at 1:100 dilution.
