GTX24800

antibody from GeneTex

Targeting: PTK2B CADTK, CAKB, FAK2, PTK, PYK2, RAFTK

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX24800

Provider

GeneTex

Proper citation

GeneTex Cat#GTX24800, RRID:AB_380429

Product name

PYK2 (phospho Tyr402) antibody

Antibody type

Polyclonal

Reactivity

Human, Mouse, Rat, Chicken/Avian

Host

Rabbit

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Pyk2 [pY402] and [pY881] phosphospecific antibodies (GTX24801). Pyk2 [pY402] and [pY881] phosphospecific antibodies (GTX24801). Increased Pyk2 phosphorylation by SDF-1a treatment (5 min) of Reh cells, detected using Pyk2 [pY402] and Pyk2 [pY881] PSSAs. (GTX24801).

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Image shows auto-phosphorylation in TGF-beta treated NM?MG cells using Pyk2 [pY402] PSSA. (GTX24800)

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunofluorescence analysis of PYK2 [pY402] was done on 70% confluent log phase A549 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton? X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with PYK2 [pY402] Rabbit Polyclonal Antibody at 1ug/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal? Secondary Antibody, Alexa Fluor? 488 conjugate at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade? Gold Antifade Mountant with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor? 555 Rhodamine Phalloidin. Panel d is a merged image showing cytoplasmic localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.

Flow cytometry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Flow cytometry analysis of PYK2 [pY402] was done on PC-3 cells treated with Pervanadate (100uM, 1 hour). Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton? X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature. Cells were labeled with PYK2 [pY402] Rabbit Polyclonal Antibody ( red histogram) or with rabbit isotype control (pink histogram) at 3-5 ug/million cells in 2.5% BSA. After incubation at room temperature for 2 hours, the cells were labeled with Alexa Fluor? 488 Goat Anti-Rabbit Secondary Antibody at a dilution of 1:400 for 30 minutes at room temperature. The representative 10,000 cells were acquired and analyzed for each sample using an Attune? Acoustic Focusing Cytometer. The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control.