Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- GTX130887 - Provider product page
- Provider
- GeneTex
- Product name
- PYK2 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references A high-throughput pipeline for validation of antibodies.
Effects of digitoxin on cell migration in ovarian cancer inflammatory microenvironment.
Sikorski K, Mehta A, Inngjerdingen M, Thakor F, Kling S, Kalina T, Nyman TA, Stensland ME, Zhou W, de Souza GA, Holden L, Stuchly J, Templin M, Lund-Johansen F
Nature methods 2018 Nov;15(11):909-912
Nature methods 2018 Nov;15(11):909-912
Effects of digitoxin on cell migration in ovarian cancer inflammatory microenvironment.
Trenti A, Boscaro C, Tedesco S, Cignarella A, Trevisi L, Bolego C
Biochemical pharmacology 2018 Aug;154:414-423
Biochemical pharmacology 2018 Aug;154:414-423
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- PYK2 antibody detects PYK2 protein by western blot analysis. Various whole cell extracts (30 £gg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PYK2 antibody (GTX130887) diluted at a dilution of 1:1000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various tissue extracts (50 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PYK2 antibody (GTX130887) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- PYK2 antibody detects PYK2 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: PYK2 protein stained by PYK2 antibody (GTX130887) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of PYK2 protein from A431 whole cell extracts using 5 £gg of PYK2 antibody (GTX130887).Western blot analysis was performed using PYK2 antibody (GTX130887).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- PYK2 antibody detects PYK2 protein at cytoplasm and nucleus in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. PYK2 antibody (GTX130887) diluted at 1:500.