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- Antibody Data
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- References [1]
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- Western blot [4]
- Immunohistochemistry [1]
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- Product number
- NBP1-91228 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-91228, RRID:AB_11033181
- Product name
- Rabbit Polyclonal PYK2/FAK2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human, rat PYK2/FAK2 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Chemotherapy-Induced Ca2+ Release Stimulates Breast Cancer Stem Cell Enrichment.
Lu H, Chen I, Shimoda LA, Park Y, Zhang C, Tran L, Zhang H, Semenza GL
Cell reports 2017 Feb 21;18(8):1946-1957
Cell reports 2017 Feb 21;18(8):1946-1957
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PYK2/FAK2 Antibody [NBP1-91228] - Analysis in human cell lines A-431 and HEK293 using anti-PTK2B antibody. Corresponding PTK2B RNA-seq data are presented for the same cell lines. Loading control: anti-GAPDH.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PYK2/FAK2 Antibody [NBP1-91228] - Human breast cancer cell line MDA-MB-231 was treated with carboplatin for 72 hours and the expression of phospho-PYK2 and total PYK2 were detected by Western blot. Image from verified customer review.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PYK2/FAK2 Antibody [NBP1-91228] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PYK2/FAK2 Antibody [NBP1-91228] - Analysis using Anti-PTK2B antibody NBP1-91228 (A) shows similar pattern to independent antibody NBP1-91227 (B).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PYK2/FAK2 Antibody [NBP1-91228] - Staining of human cerebral cortex shows strong nuclear positivity in neuronal cells.
