AM08449PU-N

antibody from Acris Antibodies GmbH

Targeting: STAT5A MGF, STAT5

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AM08449PU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AM08449PU-N, RRID:AB_2035991

Product name

anti STAT5 / STAT5A pTyr694

Antibody type

Monoclonal

Antigen

Synthetic peptide corresponding to residues surrounding Tyr694 of Mouse STAT5a protein.

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

5F6.F1

Vial size

0.1 mg

Concentration

1.0 mg/ml (by UV absorbance at 280 nm)

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western blot using AM08449PU STAT5 pTyr694 antibody shows detection of phosphorylated Stat5 (indicated by arrowhead at ~91 kDa) in NK92 cells after 30 min treatment with 1Ku of IL-2 (Lane 2). No reactivity is seen for non-phosphorylated Stat5 in untreated cells (Lane 1). The membrane was probed with the primary antibody at a 1/1,000 dilution, overnight at 4°C. For detection DyLight™800 conjugated Goat anti-Mouse IgG was used at a 1/20,000 dilution for 30 min at RT followed by visualization using a VersaDoc™ MP 4000 imaging system (Bio-Rad).

Immunocytochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

AM08449PU-N STAT5 pTyr694 antibody staining of 3T3 cells by Immunofluorescent STED Microscopy. Red represents Stat5 pTyr694 protein. Green represents Tubulin.

Immunohistochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Immunohistochemistry using AM08449PU STAT5 pTyr694 antibody shows detection of phosphorylated STAT5 pTyr694 in Human breast tissue (40X). The antibody was used a dilution to 20 μg/mL. The image shows breast epithelium with moderate nuclear staining. Tissue was Formalin Fixed, Paraffin Embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.Personal communication, Andrew Elston, Lifespan Biosciences, Seattle, WA.