NBP2-67460

antibody from Novus Biologicals

Targeting: BAK1 BAK, BCL2L7, CDN1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP2-67460

Provider

Novus Biologicals

Product name

Rabbit Monoclonal BAK Antibody

Antibody type

Monoclonal

Description

Protein A purified.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 ul

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

YWHAZ amplification/overexpression defines aggressive bladder cancer and contributes to chemo-/radio-resistance by suppressing caspase-mediated apoptosis.

Yu CC, Li CF, Chen IH, Lai MT, Lin ZJ, Korla PK, Chai CY, Ko G, Chen CM, Hwang T, Lee SC, Sheu JJ
The Journal of pathology 2019 Aug;248(4):476-487

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: BAK Antibody (SU32-07) [NBP2-67460] - Analysis of Bak on different lysates using anti-Bak antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: Human skeletal muscle Lane 3: Ags

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: BAK Antibody (SU32-07) [NBP2-67460] - Analysis of paraffin-embedded mouse lung tissue using anti-Bak antibody. Counter stained with hematoxylin.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: BAK Antibody (SU32-07) [NBP2-67460] - Analysis of paraffin-embedded human lung tissue using anti-Bak antibody. Counter stained with hematoxylin.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: BAK Antibody (SU32-07) [NBP2-67460] - Analysis of NIH/3T3 cells with Bak antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.