Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
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Validation data
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- Product number
- LS-B653 - Provider product page
- Provider
- LSBio
- Product name
- IHC-plus™ RELA / NFKB p65 Antibody (C-Terminus) LS-B653
- Antibody type
- Polyclonal
- Description
- Delipidated and defibrinated
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Storage
- Store vial at -20°C prior to opening. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunoblot of HeLa cell extract using anti-NFkb p65 (RelA) at a 1:5000 dilution. A predominant band ~65 kDa (arrowhead) corresponding to NFkb p65 Rel A is observed
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Anti-NFkb p 65 Rel A Antibody - Western Blot. Western blot of HeLa cell extract. A predominant band ~65 kD (arrowhead) corresponding to NFkb p65 Rel A is observed. All incubations except color development were performed using TBS supplemented with 0.1% Tween-20 at room temperature. The membrane was blocked in 5% dry milk for 2 h. After washing, a 1:5000 dilution of the primary antibody was added to the membrane and incubated for 2 h. Washes with buffer were performed 4 times for 5 each. The western blot was incubated with secondary antibody (HRP Goat-a-Rabbit IgG [H&L]) diluted 1:2000 for 1 h. Washes with TBS preceded color development.