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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- GTX48873 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX48873, RRID:AB_11167596
- Product name
- NFkB p65 (phospho Thr254) antibody
- Antibody type
- Polyclonal
- Host
- Rabbit
- Vial size
- 100 µl
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Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- GeneTex Goat anti Uricase antibody (GTX48873) was used to detect purified Uricase under reducing and non-reducing conditions. Samples of ~1 and 0.25 ug of protein per lane were run by SDS-PAGE and reduced samples of purified Uricase contained 4% BME and were boiled for 5 minutes. Protein was transferred to nitrocellulose and probed with Goat anti Uricase (1:5K in blocking buffer, on 4 C). Primary antibody was detected with GeneTex Dylight 649 conjugated Donkey anti Goat (1:10K 1.5 hr) RT in blocking buffer.
- Validation comment
- WB
