PA5-34737

antibody from Invitrogen Antibodies

Targeting: RELA NFKB3, p65

Immunoprecipitation

Antibody data

Submit

Validation data

Product number: PA5-34737 - Provider product page
Provider: Invitrogen Antibodies
Product name: NFkB p65 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant protein fragment
Description: Recommended positive controls: 293T, A431, HeLa, HepG2, Neuro2A, NIH-3T3, Raw264.7, C2C12, PC-12, Rat2, GFP-human NF-kappa-B-transfected 293T. Predicted reactivity: Mouse (96%), Rat (99%), Pig (98%), Chimpanzee (100%), Bovine (100%). IHC notes, Requires antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0) Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 1.37 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RELA protein structure - PA5-34737 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of NFkB p65 using Non-transfected (-) and NFkB p65 -transfected (+, including GFP-tag) 293T whole cell extracts (15 µg). Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a NFkB p65 polyclonal antibody (Product # PA5-34737) at a dilution of 1:5000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of NFkB p65 using A) 30 µg GL261 whole cell lysate (B) 30 µg C8D30 whole cell lysate and C) 30 µg NIH-3T3 whole cell lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a NFkB p65 polyclonal antibody (Product # PA5-34737) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of NFkB p65 using 30 µg of MOLT4 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a NFkB p65 polyclonal antibody (Product # PA5-34737) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of NFkB p65 was performed by separating 15 µg of non-transfected (-) and NFkB p65 -transfected (+) (including GFP-tag) 293T whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a NFkB p65 Polyclonal Antibody (Product # PA5-34737) at a dilution of 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of NFkB p65 was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a NFkB p65 Polyclonal Antibody (Product # PA5-34737) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of NFkB p65 was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a NFkB p65 Polyclonal Antibody (Product # PA5-34737) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of NFkB p65 was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a NFkB p65 Polyclonal Antibody (Product # PA5-34737) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry (Paraffin) analysis of NFkB p65 was performed in paraffin-embedded mouse cervix tissue using NFkB p65 Polyclonal Antibody (Product # PA5-34737) at a dilution of 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry (Paraffin) analysis of NFkB p65 was performed in paraffin-embedded rat liver tissue using NFkB p65 Polyclonal Antibody (Product # PA5-34737) at a dilution of 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry (Paraffin) analysis of NFkB p65 was performed in paraffin-embedded mouse kidney tissue using NFkB p65 Polyclonal Antibody (Product # PA5-34737) at a dilution of 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry (Paraffin) analysis of NFkB p65 was performed in paraffin-embedded mouse lung tissue using NFkB p65 Polyclonal Antibody (Product # PA5-34737) at a dilution of 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry (Paraffin) analysis of NFkB p65 was performed in paraffin-embedded rat lung tissue using NFkB p65 Polyclonal Antibody (Product # PA5-34737) at a dilution of 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.